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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: magickspore]
#19484870 - 01/28/14 05:47 AM (10 years, 3 days ago) |
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kratom, i agree with jj and majik, go for around 8:30-11o clock.
as for what size u should let it grow out, i think the first plate (the one spores were either swabbed/swiped/syringe dropped/etc) is the one that u should transfer immediately from, because that is the plate most likely to have an outside contaminate on it from whatever the spore source was. anything after that 1st plate, i just make sure it doesn't get to the edge of the plate. can be dime sized, quarter, half dollar, bigger, etc. early on (2nd plate, sometimes even first if u were lucky enough to just get a couple spores on), the more it grows out, the more likely u r to see sectoring. and as JJ said, sometimes i also go back to a plate i already transferred from after it has grown out over the wedge hole if it looks like there are some sectors/growth that look(s) good. so basically, after the first initial spore deposit plate, as long as its not contaminated, and as long as it is not hitting the perimeter of the dish, let ur plates go wild! (but thats just me)
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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kratom_redmomd
Kratom Lover



Registered: 07/25/13
Posts: 411
Loc: Hiking Cascadia
Last seen: 4 years, 3 months
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19485389 - 01/28/14 09:37 AM (10 years, 3 days ago) |
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Thanks for the replys. I'll have to go back and see what I transfered. I was trying to work fast. Well, I was so fast that I forgot to flame my exacto the first time. Caught myself before I got it off the plate. So then I flamed it and went for another edge. After looking at the pictures I saw where I think I should have taken it from. So far, so good.
-------------------- "If the goal is to ride the bike, why is everyone arguing about which bike to ride? We are all bike riders. Ride your bike." -Unknown If I was going to destroy a nation, first I would destroy its language. -Confusious ¿Hablas Inglés?
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magickspore



Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 8 months, 4 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: kratom_redmomd]
#19485573 - 01/28/14 10:41 AM (10 years, 3 days ago) |
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So I feel like sharing my frustration with you good people. I went out and bought a dehydrator a few min ago and after acting like a kid on Christmas playing with his new toy. I proceeded to take my fresh picks and load my new toy. Well to my dismay some of my fruits are to small to sit on the racks. Not all mind you but alot fml
Haha well now I look forward to my next failure, I mean grow... Also I checked on my botched clone job and it is over ran with bacteria. So attempt 2 will be later. This time I'm just gonna drop a pin right on the dish and also take a clone from a larger fruit.
Edited by magickspore (01/28/14 10:44 AM)
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: magickspore]
#19485587 - 01/28/14 10:43 AM (10 years, 3 days ago) |
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haha like a kid at christmas!
if they are that small, air drying should be fine. i don't even have a dehydrator, i air dry all mine, just takes longer.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: magickspore]
#19485597 - 01/28/14 10:46 AM (10 years, 3 days ago) |
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What they said about agar, The very bottom tray is where all the small mushrooms go, no fail 
It's like 63F in here so my latest transfers haven't really done much yet.
The Pan spores were put to grain and agar on the 21st I believe, The FL I see maybe some germination and one of the dishes has some contam spots appearing now and no growth on the grains yet, the RDU has yet to germinate on any dish and the grain jar has something that doesn't look healthy starting to grow, the tropics have yet to germinate on either the petri or the grain. And the reishi is going slowly on the dishes and slowly on the grains.
F-winter.
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magickspore



Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 8 months, 4 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19485601 - 01/28/14 10:46 AM (10 years, 3 days ago) |
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Quote:
blindingleaf said: haha like a kid at christmas!
if they are that small, air drying should be fine. i don't even have a dehydrator, i air dry all mine, just takes longer.
Yea I've been using my fan and a lid from a tote it works well. It was mostly the disappointment.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: magickspore]
#19485624 - 01/28/14 10:53 AM (10 years, 3 days ago) |
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Quote:
F-winter.
QFT!! also still waiting on transferred wedges to grow out cause of the temp! but I'm loving what its doing to my stuff in fruiting...(weight wise)
only thing i have heard that is good about colder months (dunno if this is true or not, but makes sense) is the ambient air contam/spore load is less. once summer hits, weather is better for contaminate to germinate/sporulate in and around house/apt, and can carry into projects. i think it was an RR quote, but not sure. seems logical, but still... f- winter!
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Gorlami



Registered: 08/28/13
Posts: 328
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19485641 - 01/28/14 10:57 AM (10 years, 3 days ago) |
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Quote:
blindingleaf said:
Quote:
F-winter.
QFT!! also still waiting on transferred wedges to grow out cause of the temp! but I'm loving what its doing to my stuff in fruiting...(weight wise)
only thing i have heard that is good about colder months (dunno if this is true or not, but makes sense) is the ambient air contam/spore load is less. once summer hits, weather is better for contaminate to germinate/sporulate in and around house/apt, and can carry into projects. i think it was an RR quote, but not sure. seems logical, but still... f- winter!
I think i'm going to subject some agar to the outside world to see what's up.
Probably should do a control experiment inside as well for the same amount of time. 
You guys think 10 seconds is enough? And should I turn off any fans that I have inside before?
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magickspore



Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 8 months, 4 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19485649 - 01/28/14 10:59 AM (10 years, 3 days ago) |
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Quote:
bodhisatta said: What they said about agar, The very bottom tray is where all the small mushrooms go, no fail 
It's like 63F in here so my latest transfers haven't really done much yet.
The Pan spores were put to grain and agar on the 21st I believe, The FL I see maybe some germination and one of the dishes has some contam spots appearing now and no growth on the grains yet, the RDU has yet to germinate on any dish and the grain jar has something that doesn't look healthy starting to grow, the tropics have yet to germinate on either the petri or the grain. And the reishi is going slowly on the dishes and slowly on the grains.
F-winter.
WOW I'M AN IDIOT! Thanks bodhi
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: magickspore]
#19485666 - 01/28/14 11:01 AM (10 years, 3 days ago) |
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10s should be fine. Interesting to see if anything happens to the outside plate since it's so cold.
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Gorlami



Registered: 08/28/13
Posts: 328
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19485702 - 01/28/14 11:09 AM (10 years, 3 days ago) |
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Quote:
bodhisatta said: 10s should be fine. Interesting to see if anything happens to the outside plate since it's so cold.
I'm assuming it will be contaminated, however there's no way of knowing for sure if it's going to be less than in summer... It won't grow in those conditions, but they probably lay dormant until their environment is ideal.
EDIT: Done and done, I'll keep y'all posted when I have results!
Edited by Gorlami (01/28/14 11:16 AM)
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Gorlami]
#19485891 - 01/28/14 11:53 AM (10 years, 3 days ago) |
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yea id guess both would contam, but ud' see more scattered spots where they landed in the summer air plate, indicating higher spore load in ambient air, but thats just a guess if that indeed is true
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Gorlami



Registered: 08/28/13
Posts: 328
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19486428 - 01/28/14 02:12 PM (10 years, 2 days ago) |
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Bit of an update. I'm not sure how far i'm from an isolate on the 3rd/4th transfer, but i'm finally confident my plates are at least out of harms/contaminates way.
No sectoring or rhizomorphic growth as of yet.
Texas is on 4th transfer. (was dirtiest of all.)
Z Strain is on 3rd transfer.
Cloned of a pin on fudged up monotub that contamned is on 3rd transfer -> transfered to 2 dishes. (best growth displayed so far)
When I have enough colonization on one clean plate of agar I'm going to inoculate a couple of jars so I can have a project in progress while I narrow down these guys down to isolates that I can test. I don't care about the crapshoot genes, I just want to trip. 
Wish I could show you guys some pictures, but alas, 1/2 pints with frosted glass are garbage for viewing growing myc.
I just bought some Ziploc Twistcap containers, those should be better by a narrow margin.
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 2 months
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Gorlami]
#19486492 - 01/28/14 02:32 PM (10 years, 2 days ago) |
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Can you post all your agar work here, or is this a grow along for spore to agar work? I poured my first plates about a week or so ago and im seeing some nice growth...
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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KnownPown
Fathead Shroomer



Registered: 10/27/12
Posts: 219
Loc: Washington State
Last seen: 6 years, 6 months
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: 1down5up]
#19486849 - 01/28/14 03:45 PM (10 years, 2 days ago) |
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I've got 7 grain masters started, including one EQ variety. B+ is on its 3rd transfer from spores, will keep on trying to isolate while at least in the meantime I got something started!
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: 1down5up]
#19487078 - 01/28/14 04:31 PM (10 years, 2 days ago) |
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Quote:
1down5up said: Can you post all your agar work here, or is this a grow along for spore to agar work? I poured my first plates about a week or so ago and im seeing some nice growth...
you sure can, I'm just posting my log here, that way people can ask questions about any one of the steps. If you're not where I am along the spore to spore path no worry you can still come here to get help etc..
To whale and known
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 2 months
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19487121 - 01/28/14 04:39 PM (10 years, 2 days ago) |
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I'll upload as soon as the camera's ready....thanks man
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: 1down5up]
#19488210 - 01/28/14 07:42 PM (10 years, 2 days ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: cronicr]
#19488231 - 01/28/14 07:47 PM (10 years, 2 days ago) |
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boards have been quiet, no? i'd post pics of my KSSS for this thread, but not much has changed since last pic. got 100 petris in the mail today though
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19488240 - 01/28/14 07:49 PM (10 years, 2 days ago) |
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Quote:
blindingleaf said: boards have been quiet, no?
i just got here
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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