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InvisibleFrankHorrigan
The Inquisition
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
    #19447198 - 01/20/14 11:30 AM (10 years, 11 days ago)

:hi:

Yes please! I totally spaced out :rofl:

It's been a (pleasantly) busy start to the year in my house.

I'll :pm: you later today, got a couple more harvests to take care of first. 


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


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InvisiblebodhisattaMDiscordReddit
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: FrankHorrigan]
    #19447199 - 01/20/14 11:31 AM (10 years, 11 days ago)

Quote:

FrankHorrigan said:
:hi:

Yes please! I totally spaced out :rofl:

It's been a (pleasantly) busy start to the year in my house.




Ha, I figured. Some times I get busy and don't feel like explaining my life situations to a bunch of on-line people :lol:


Just shoot me a PM


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InvisibleGorlami
I'm a teapot User Gallery


Registered: 08/28/13
Posts: 328
Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: FrankHorrigan]
    #19449320 - 01/20/14 07:18 PM (10 years, 10 days ago)

Quote:

blindingleaf said:
yea u r right in thinking that!

the more spores u put down, the more germinate, the more they mate, creating dikaryons that branch out.  u want to limit that branch out, ideally in a perfect phillip k dick world, we could all get a print/syringe with 2 spores that mate and create an instant strain, but we don't.  so when we noc up a plate with a drop of syringe or a little scrape of print, or a swab of a q tip, who knows how many spores just got on that plate (i don't but I'm sure philip k dick RIP does or those who use Substance D :smile: ) ?  the less the better (as far as sectoring is concerned and our purposees here).  the fewer spores on the initial plate will mean less mating, less sectoring, less "jumbled up white cottony mess" (just on the initial plate if it is not grown out a long time, and sometimes even IF IT IS grown out to the perimeter of the plate).  perhaps, from the situation u describe, the 2 plates that DO display thicker growth coincidentally had fewer spore deposits on them...? 
as far as condensation dispersing the spores...not sure, never heard that or read about it so cannot comment on it (though i doubt it unless the "condensation" dropped onto the plate after it was nocced with spores and spread them around somehow whilst moving/handling the plate...but even then...would not affect the amount of spores on plate, just the amount of germinating spores reaching each other to mate/create dikaryons).  I'm no scientist though, so hope that helps a little.

yes waiting is hard.  to quote mr roger's neighborhood: (i hope SOME of u remember this episode, I still do)
"it's very very very hard...to wait.  especially when ur waiting....for something...very nice!  It's very very very....hard to wait"  haha love that song.




Hah, having 2 spores in a syringe would be horrible.  Unless they were the most genetically awesome... even then, that would take the fun out of doing agar. :P

When I was mentioning condensation, I meant that there could have been drops on the underside of the lid that were dropping onto my agar making it wet, handling the petri would roll that drop around and effectively disperse the spores from edge to edge.  But as Frank said, maybe that doesn't happen since when the spores are laid, the agar itself is sticky.  I would suspect that the water in the spore syringe would have that effect and drag the spores all over the plates when tilted and handled.







Quote:

FrankHorrigan said:
Having a single strain formed from an MS inoculation (in the Dick world) would be so annoying...more often than not, strains formed during MS are not worth keeping.

As for your plates Whale, be patient. If you are not sure what you are looking at, take transfers to new plates. An MS inoculation always makes a "messy" plate, a transfer will show you what you've got in a few days time.

Condensation does not "spread" spores around, your agar media is sticky. Condensation on the lid does little harm, but you should try to minimize it. Keep your petris inside an insulated lunchbox while they are not in use. This protects it from temp swings, which cause condensation. Droplets that form and fall to the agar itself can carry contams from the lid as well.

:goodluck:





Thanks for the input, I'm starting to think I'm transfering contaminations from plate to plate and from plate to WBS.

I decided to open one of the plates to show you guys.



I only really realized how dire it looked once I opened the plate.  These 1/2 pints suck for viewing.  It's embarassing really, I think i've been transfering garbage lol.  It's a gooey mess and it doesn't smell like mycelium. :frown:  Thinking I got some bunk syringes...  :cussing:  :pottymouth:  :lame: 

:irdumbtard:

The plates looked fine before inoculation and were stored properly for 2-3 days before use.

The Texas spores seem to be doing fine, and they were from the same vendor. :S

What do you guys think?

:badmanthisfeels:


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InvisibleJ. Jack Flash
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Registered: 11/20/13
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Gorlami]
    #19449427 - 01/20/14 07:44 PM (10 years, 10 days ago)

looks ill... in the traditional sense.  sorry man.

couple questions for you.  do you have a still air box? i assume you do.  do you still have the plastic discs you cut out?  for ease of viewing your agar plates, you can cut the discs down to the size of a canning lid and us it instead, to make for a nice window.  i've done this one time and it worked a treat. 

can't really see through the lid here, but it is lit through the lid.

it even helped me decide to cut the top (bottom) out of my SAB to make a pile more of them.  one interesting benefit i noticed is that they do not seal air tight, so you can drain the condensation from it as it forms.  the ring in my case did a fine job keeping contaminants out. 

just sayin.


--------------------
 
the j stands for jesus.
2020 new years grow along


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InvisibleGorlami
I'm a teapot User Gallery


Registered: 08/28/13
Posts: 328
Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: J. Jack Flash]
    #19449454 - 01/20/14 07:51 PM (10 years, 10 days ago)

Yes sir I do have a SAB, I cut squares for the arm holes, but I see what you mean.  This would be a problem for me since I don't pour my agar in the SAB, instead I pour inside the jars, then I pressure cook them.  I leave them cool naturally and theres no visible pooling of water, there is condensation on the underside of the lid though.

Thanks for sharing. :smile:


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Gorlami]
    #19449512 - 01/20/14 08:02 PM (10 years, 10 days ago)

I took a print recently and will be putting it to agar tonight.  IIRC it was/is a mazatapec.  I would expect 3-5 xfers from this than off to the grain factory for approval.


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InvisibleJ. Jack Flash
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Gorlami]
    #19449609 - 01/20/14 08:23 PM (10 years, 10 days ago)

sure man.  i'll clarify that i've only done no-pour like you.  usually those twistloc containers.  sometimes there's no condensation, other times, there's tons.  one day i'll figure it out.

SCIENCE!

hypnotoad?  zat you, whippy?


--------------------
 
the j stands for jesus.
2020 new years grow along


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Invisibleblindingleaf
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: J. Jack Flash]
    #19449740 - 01/20/14 08:51 PM (10 years, 10 days ago)

to help with condensation on no pours (any tek)  after u heat up the agar and pour into the dishes, leave lid slightly loose so steam can escape and let them fall back to room temp before pc'ing.  u will only get a little condensation this way, but not nearly as much, easier viewing!


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: J. Jack Flash]
    #19449746 - 01/20/14 08:52 PM (10 years, 10 days ago)

Quote:

J. Jack Flash said:

hypnotoad?  zat you, whippy?



:ruggedwink:


Prints to agar.  Awaiting the inevitable spores-on-agar cluster-fuck.  I also did some portabello prints.  I have finally learned enough about agar to try and revive my ports.....Next time I wont run out my master jar lol.


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Offlinekratom_redmomd
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: HypnotoadCroaked]
    #19449944 - 01/20/14 09:26 PM (10 years, 10 days ago)

I have what looks like myc on my agar!
:dancer: :dancer: :dancer:

I might try getting a pic or two in my SAB later tonight (unless someone suggests that I don't).

Fiiiiiinally.


--------------------
"If the goal is to ride the bike, why is everyone arguing about which bike to ride?  We are all bike riders.  Ride your bike." -Unknown

If I was going to destroy a nation,
first I would destroy its language. -Confusious
¿Hablas Inglés?




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InvisiblebodhisattaMDiscordReddit
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: kratom_redmomd]
    #19450010 - 01/20/14 09:42 PM (10 years, 10 days ago)

Reishi agar wedges to grain today
Pan Cyan tropical spores to agar and grain today
Pan Cyan Fl wild print to agar and grain today
Pan Cyan RTU Australian spores to agar and grain today
Thanks to Sgt and Chron.

sorry for the small update, I'll also be transferring dishes tomorrow of the creeper relevant to this thread


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OfflineSgt. Pepper
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
    #19450110 - 01/20/14 09:59 PM (10 years, 10 days ago)

Quote:

bodhisatta said:
Reishi agar wedges to grain today
Pan Cyan tropical spores to agar and grain today
Pan Cyan Fl wild print to agar and grain today
Pan Cyan RTU Australian spores to agar and grain today
Thanks to Sgt and Chron.

sorry for the small update, I'll also be transferring dishes tomorrow of the creeper relevant to this thread



Glad to see those pan spores going to use! One thing though and I don't mean to sound like a dick, but they were given to me as pan tropicalis spores, not pan cyan tropical. It's a whole 'nother species, however they are macroscopically identical.


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InvisiblebodhisattaMDiscordReddit
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Sgt. Pepper]
    #19450254 - 01/20/14 10:23 PM (10 years, 10 days ago)

Quote:

It's a whole 'nother species, however they are macroscopically identical.



Noted, I'll refer to them as such from this point out and label them as such too. I didn't know. I'll have to go do some researching. Pan Tropicals are on grain and agar*


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OfflineSgt. Pepper
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
    #19450291 - 01/20/14 10:29 PM (10 years, 10 days ago)

Quote:

bodhisatta said:
Quote:

It's a whole 'nother species, however they are macroscopically identical.



Noted, I'll refer to them as such from this point out and label them as such too. I didn't know. I'll have to go do some researching. Pan Tropicals are on grain and agar*



There's not a whole lot to research to be honest, unless you're into microscopic mycology. Most of what the search engine pulls up is threads from 2006 where they argue about whether or not it's even a distinct species. It is grown exactly the same as pan cyans, just it's spores will look different under a microscope.


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Invisiblebootster
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Sgt. Pepper]
    #19450774 - 01/21/14 12:11 AM (10 years, 10 days ago)

Thanks for all of your hard work guys. I've got some Pan Cyans that are in the grain stage (syringes from a trusted vendor/supporter). I have some on agar and some inoced through a port. I hate to do it that way but spring is coming on fast. The single drops on agar are in grains too. :bow: to the agar.

I'll let the "Dick world comment" go with a grain of salt for now, crass as it may be, futile at best.


Edited by bootster (01/21/14 01:30 AM)


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Invisibleblindingleaf
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bootster]
    #19451289 - 01/21/14 03:16 AM (10 years, 10 days ago)

phillip k dick world was really meant as an analogy, i really didn't mean to rustle any feathers with it.  I'm sorry if i did.  :shrug:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlinemagickspore
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
    #19451370 - 01/21/14 04:28 AM (10 years, 10 days ago)

Just took my 2nd transfer of Aussie.
Made a new gym plate other was only trich lol.
Got germination on my PB, EQ, and KSSS.

Rant/Vent
I've had knotts since Sunday. If I dont see pins or at least primordia(I think that's the right word/spelling) by Tues. night, Wed. morning.  I'm gonna give nature a piece of my mind. I can't take the suspense! Just fucking fruit already. /rant/vent]

So how is everyone else?


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Invisibleblindingleaf
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: magickspore]
    #19451408 - 01/21/14 04:49 AM (10 years, 10 days ago)

right now I'm too  :raveface:

but later (probably after i wake uo) i will cook some pasty plates (already prepped and rdy to load. 15 of these: )


and transfer to them... creeper (bodhi what what!), KSSS (will o what what!), burma, and shiitaki grocery clone, with 5 extra plates to spare for cloning pins from a troublesome PR grow.

bodhi, ur swab (both ends) was super clean, was that from a mono grow?


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisiblebodhisattaMDiscordReddit
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
    #19452128 - 01/21/14 10:13 AM (10 years, 10 days ago)

Quote:

bodhi, ur swab (both ends) was super clean, was that from a mono grow?




If you look through my gallery you'll see some petri dishes with shrooms growing on them. They're basically in-vitro fruits. I used those since I figured they would be way cleaner than a fruit from the FC.


Good to know about the tropics Sgt.

I'm excited about this reishi grow now haha. More transfers for creeper dishes for this thread in a day or two probably if not today if I get off my ass.


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OfflineSgt. Pepper
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
    #19452137 - 01/21/14 10:15 AM (10 years, 10 days ago)

Haha. I'm loving the reishi's so far, they look nice and dense on agar. I'm seeing a small amount of growth on agar but not enough for a picture yet.


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