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OfflineFezhat
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Registered: 01/12/14
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Growth Study of Potential Cobweb Infestation.
    #19407192 - 01/12/14 07:24 AM (10 years, 19 days ago)

I've turned this post into a comprehensive, Daily study of growth for
newer growers. As one myself, perhaps this will provide some much needed insight into how and how fast a potential cobweb mold infestation grows. Photo log is below.


Photograph Log of Growth Progress:


Day Four after first Inoculation - First Signs of Growth.

Small, separate patches of wispy growth is seen around the inoculation sites. At this stage it would be difficult for anyone to diagnose a potential problem.






Day Five - Growth Spread begins above the Substrate layer.

It's becoming a little easier to see the growth now on the top half of the box. Experienced growers might just be able to identify potential contaminant growth at this stage, but I wouldn't jump the gun like I did.




Day Six.

A couple of days after first signs and it's spread fairly quickly across more substrate. If your cake is contaminated, any experienced growers would likely tell you to scrap the contaminated grow to save any others, with proper disposal procedures, of course.






Day Seven - Growth Spread picks up speed.

The Large patch that first spawned has thickened up a little, and a few strands have grabbed hold of the plastic outer casing, reminiscent of Mildew. Contamination has also started to pop up in other areas of the grow box. I also noticed what seemed to be the original Cubensis Mycelial growth in one of the corners of the cake, which I've highlighted in red for ID/confirmation.

This is getting to be an interesting growth study, and I've certainly learned a thing or two. Perhaps my good friend SWIM will have to invest in a PC, some bulk 200L vermiculite, 100L Perlite and some 5kg bags of BRF, and begin his own PF Jar grows!










Day Eight.






Day Nine.








Day Ten.

Unfortunately I've been too busy today to snap a photo of today's growth progress!


Day Eleven.

No significant growth changes were seen.


Day Twelve.

Growth of the cobweb infestation increased significantly overnight.




Day Thirteen.

Mycelial growth has been spotted on other parts of the cake. Cobweb infestation has thickened a little.








Day Fourteen.

No significant changes were spotted.


Day Fifteen.

I thought the growth had not progressed much over the past few days. Coming in today, I took a look at the sides of the box and boy was I wrong! Many pictures below!






It looks like there's more contaminants starting to emerge now that the temperature's down to 20, enough to have slowed the growth of the mycelium to approach a stand-still. Seems they're seizing their opportunity. Prepare for war!

What could be the start of a lovely Trich outbreak soon to come.




Totally stumped as to what this is. Could be the result of bacteria, but unsure. Please leave a comment below if you might know!






Here's the Mycelium sat in the corner here. Farewell, sweet Cubensis! (P.S. I'm now unsure if this is actually Mycelium, and not just some other contam!)




Day Sixteen.

Doing a Peroxide Purge of H2O2 susceptible Mold(s) to allow other contaminants room to grow. I am interested in seeing what else might have been present in the Substrate that I have not been able to see. I also agitated the dry layer before I opened this for purging in a clean environment so as to inspect for new contaminant growth.

I also spotted a small patch of yellow mold growth, which I've posted a couple of pictures of below. It's hard to see, so I've circled it for you all.






And some weird shit I saw on the bottom of the cake. I dunno what this is, so please, if you know, enlighten me!




P.S. For those who posted on the original post regarding identification, please do feel free to continue offering advice and ID help on this forum thread, I feel it'll help immensely!

P.P.S Steam Sterilization stinks! Save yourself time and money and buy a good Pressure Cooker!


Edited by Fezhat (01/25/14 09:10 AM)


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OfflineRauhfasertapete
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Re: Possible Contaminant - ID Needed! [Re: Fezhat]
    #19407209 - 01/12/14 07:30 AM (10 years, 19 days ago)

Patience: wait until you can clearly see what´s going on.

If there´s a contamination, you can´t do anything anyways.


--------------------
Ich will Eins werden mit dem Gewürm auf dem Felde!


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OfflineMr.Sir
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Registered: 09/28/13
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Re: Possible Contaminant - ID Needed! [Re: Rauhfasertapete]
    #19407320 - 01/12/14 08:19 AM (10 years, 19 days ago)

hard to tell with the pictures and the super-coarse verm... looks like a contam though, keep an eye on it post more pics if it doesn't become obvious...


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OfflineFezhat
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Re: Possible Contaminant - ID Needed! *DELETED* [Re: Mr.Sir]
    #19412183 - 01/13/14 12:01 PM (10 years, 17 days ago)

Post deleted by Fezhat

Reason for deletion: Not Needed.



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OfflineRauhfasertapete
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Registered: 02/22/13
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Re: Possible Contaminant - ID Needed! [Re: Fezhat]
    #19412233 - 01/13/14 12:17 PM (10 years, 17 days ago)

H2O2 would do the the same damage to the mycelium of the psilocybe as it would do to the mycelium of the other stuff youve got there. and if these hyphae already come out of the vermiculite, they are already everywhere inside the substrate. there is no remedy. throw it away.


--------------------
Ich will Eins werden mit dem Gewürm auf dem Felde!


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OfflineKizzle
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Re: Possible Contaminant - ID Needed! [Re: Rauhfasertapete]
    #19412395 - 01/13/14 01:10 PM (10 years, 17 days ago)

Cladobotryum only grows across the surface. There are other grey molds like Mucor that primarily grow in the spawn and then grow out through the substrate. The first pics you posted looked fairly normal but those sparse, long strands look more like mold. It probably smells like mold too.

Oh and there are actually a number of molds that can produce strains vulnerable to peroxide. I've actually been studying the subject quite a bit. The problem is the substrate tends to protect them so it's usually not completely effective.


--------------------


Edited by Kizzle (01/13/14 01:20 PM)


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OfflineMr.Sir
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Re: Possible Contaminant - ID Needed! [Re: Fezhat]
    #19412818 - 01/13/14 03:04 PM (10 years, 17 days ago)

Quote:

Fezhat said:
New Picture posted up below. This is about 24 hours after the previous photograph, I think my suspicions are correct. I've been told it can fully colonize a grow within 48 hours, but am heading out tomorrow morning for some 3% Hydrogen Peroxide Solution.

I'm not sure if it'll get rid of the mold, but at worst it'll delay the growth and give any mycelium a chance at beating it.

I always thought that Cobweb Mold only ever grew above the substrate. Is it common for it to grow within the BRF substrate too, and are there any other ways I could combat this problem?





Don't open - trash it


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OfflineFezhat
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Registered: 01/12/14
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Re: Possible Contaminant - ID Needed! [Re: Mr.Sir] * 1
    #19415978 - 01/14/14 05:54 AM (10 years, 17 days ago)

Alright, I've come to a decision :laugh:.

While I'm in a little dismay that this is probably a throw-away project, and a little disheartening as a new grower, I figured I'd make this post of some use to Shroomery and do a daily post-up of this for the next 30 days. This is my only cake, and it's in a sealed filter box, so no risk of outside contamination here, so I'll be trying to post Daily pictures of this growth as a study of how this spreads. I haven't managed to find a comprehensive growth scale done with cobweb mold before, and since it's a fast grower and a fairly common encounter for cultivators, I think newer growers like myself could learn a thing or two.

Weird thign is, I've heard that Dactylium is extremely aggressive, and can dominate and completely cover a grow within the space of 48 hours from first spotting it. I've taken the liberty of editing the OP above to include new pictures and the timescale for everyone.

And hell, if it even turns out just to be wispy Cubensis mycelium, even better, right?


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OfflineRauhfasertapete
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Registered: 02/22/13
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Re: Possible Contaminant - ID Needed! [Re: Fezhat]
    #19416014 - 01/14/14 06:09 AM (10 years, 17 days ago)

the term "cobweb" does not really denote a species, as there are lots of molds that look like that; probably you will never find out what it actually is. but let it grow, and keep updating until it starts to grow fruitbodies (or conidia or whatever it may grow). There are already a few threads and journals of people who have grown some extremely nasty stuff, some of which is pretty interesting. I like that, its like being a gardener and developing an interest for strange weeds that other people would just uproot and throw away.

Go for it, whatever it is!


--------------------
Ich will Eins werden mit dem Gewürm auf dem Felde!


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OfflineRabbit88
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Re: Growth Study of Potential Cobweb Infestation. [Re: Fezhat]
    #19416936 - 01/14/14 11:26 AM (10 years, 17 days ago)

Yea I say go for ive heard of people beating cobweb with peroxide treatment ive heard its one of the few contams that arent a death sentence


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Looking for rare exotics  like the tecorums , pan tropics, or p. Hispanica pm if you got what im looking for


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OfflineRauhfasertapete
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Registered: 02/22/13
Posts: 214
Loc: Upper Franconia
Last seen: 7 years, 11 months
Re: Growth Study of Potential Cobweb Infestation. [Re: Rabbit88]
    #19416964 - 01/14/14 11:35 AM (10 years, 17 days ago)

hey, no, i meant he should grow the mold instead of the mushroom! just for the enjoyment of a wonderful healthy little mold garden


--------------------
Ich will Eins werden mit dem Gewürm auf dem Felde!


if mutual gift exchange is desired, follow this link


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OfflineFezhat
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Re: Growth Study of Potential Cobweb Infestation. [Re: Rauhfasertapete]
    #19466711 - 01/24/14 09:31 AM (10 years, 7 days ago)

Thread Bump going on, I feel this post would teach people a little!


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OfflineKizzle
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Re: Growth Study of Potential Cobweb Infestation. [Re: Fezhat]
    #19467337 - 01/24/14 12:15 PM (10 years, 6 days ago)

From afar it looks like there may be some Trich colonies or some other green mold but it's kind of hard to see clearly.

Here's something that might you identify the other growth. Look very closely for little thread like structures with white or black dots on the tip. They're a sure indication it's mold. Here's a couple pics to show you what I'm talking about


--------------------


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OfflineFezhat
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Re: Growth Study of Potential Cobweb Infestation. [Re: Kizzle]
    #19471957 - 01/25/14 12:42 PM (10 years, 5 days ago)

There weren't any distinctive pin formations on the contaminant growth to speak of. I had read the thread linked in your post, and one of the pictures seems somewhat relevant to what I was dealing with. It had begun to attach itself to the outer casing and grew solely across the substrate. There may have been some under the dry verm layer which allowed it to grow around many spots of the cake but it generally seemed to avoid the mycelium spot that had appeared.

After I decided to turn my grow project into a research mold garden, I took it off the heated water system, which dropped it down from about 27-28 Celsius to 19-20, upon which it didn't seem to spread as much, but thickened considerably. I've sprayed the whole cake with Peroxide and even moved the verm layer about to get at any if it's under there (in a clean environment with sterilized gloves, a labcoat and a breathing mask, of course) and it seems to have fizzed into nothing, or very little.


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