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OfflineAlan RockefellerM
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Re: starting a herbarium [Re: The Lightning]
    #19407757 - 01/12/14 10:38 AM (10 years, 19 days ago)

Quote:

The Lightning said:
It might be helpful to add that some collections will inevitably require DNA testing. I provided samples to a lab recently and they asked that I dehydrate the mushrooms at 105 degrees but no higher.

When you purchase a dehydrator remember to find one that has a temperature dial so you can set it to 105.






See http://www.kahaku.go.jp/research/publication/botany/download/37_3/BNMNS_B370301.pdf


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InvisibleThe Lightning
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Re: starting a herbarium [Re: Alan Rockefeller]
    #19407790 - 01/12/14 10:47 AM (10 years, 19 days ago)

:thumbup: Okay, so I stand corrected. 'Didn't know that 'til now.


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Offlineart2312
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Re: starting a herbarium [Re: The Lightning]
    #19408171 - 01/12/14 12:32 PM (10 years, 18 days ago)

So i should probably dry them at higher temps and just try and get very good pictures of the fresh specimen for records. I wanted to try and keep the specimens looking as close as possible to the fresh fruits but i'd rather try and preserve the DNA. Beautiful looking specimens are nice to look at, but if they are scientifically useless then their isn't any point in taking the fruits in the first place...


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OfflineAlan RockefellerM
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Re: starting a herbarium [Re: art2312]
    #19408358 - 01/12/14 01:24 PM (10 years, 18 days ago)

I think the most important part is that they are well dried and put into a ziplock bag, the exact temperature is secondary.  But I think it makes sense to not heat them more than necessary, since dna is a pretty fragile molecule.


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Offlineart2312
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Re: starting a herbarium [Re: Alan Rockefeller]
    #19408409 - 01/12/14 01:40 PM (10 years, 18 days ago)

no doubt! it seems like the ideal thing to do is dry them as quickly as you can without over heating them... in that link you posted before, the highest temp they dried them at was 159.8F and they got quality DNA from all of those specimens... so that'll probably be the route i take as well.


--------------------
I don't mind being ogled, ridiculed, made to feel minuscule.
If you consider the source, it's kinda pitiful
The only thing you really know about me is.....That's all you'll ever know!!!!


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