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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: Psilocybe silvatica [Re: Byrain]
#19395372 - 01/09/14 07:01 PM (10 years, 1 month ago) |
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Quote:
Byrain said: You don't use a stage micrometer to make measurements, you use it to calibrate your reticle (Or software).
How is what I'm trying to get at? I don't have a reticle so I'll probably be using Piximètre.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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I created this for my friends. I consider you one of them.
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Quote:
The Lightning said: I created this for my friends. I consider you one of them.
This is exceedingly helpful, thank you sir 
Will I still be able to make out structures (ie. cystidia, Basidia) without dye?
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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Byrain

Registered: 01/07/10
Posts: 9,664
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I've never used Piximètre, someone else certainly knows.
@The Lightning, does that actually contain a section on calibrating your scope for Piximètre? I'm not seeing it.
Quote:
maynardjameskeenan said: Will I still be able to make out structures (ie. cystidia, Basidia) without dye?
Yes, thin!!! sections are key.
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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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Re: Psilocybe silvatica [Re: Byrain]
#19395494 - 01/09/14 07:23 PM (10 years, 1 month ago) |
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Quote:
Will I still be able to make out structures (ie. cystidia, Basidia) without dye?
HI. Normally, we use the term "stain(s)" instead of dye, unless dye is being used as the stain. The best stain you can find easily might be Congo Red. It's in the link. If you're waiting for Congo Red, KOH is an awesome rehydration liquid that allows enough visibility most of the time to make observations of cystidia. I really recommend KOH and Congo Red in separate, amber dropper bottles. Check out that *ink.
Quote:
@The Lightning, does that actually contain a section on calibrating your scope for Piximètre? I'm not seeing it.
Not yet. Alan, if you would like to add that to the document, please let me know. If Alan doesn't add it, someone else feeling the need can provide the instructions without cost and I will add them.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,752
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Did anyone consider Hypholoma for this?
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nomadbrad
Oregrownian



Registered: 10/23/06
Posts: 1,160
Loc: Pacific NW
Last seen: 5 years, 4 months
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Re: Psilocybe silvatica [Re: inski]
#19396293 - 01/09/14 10:32 PM (10 years, 1 month ago) |
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Did you have a species in mind? I can't seem to find one that resembles it yet.
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Re: Psilocybe silvatica [Re: inski]
#19396846 - 01/10/14 12:37 AM (10 years, 1 month ago) |
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Quote:
inski said: Did anyone consider Hypholoma for this?
I could see that. I dont think it is a Psilocybe species myself.
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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Anglerfish
hearing things



Registered: 09/08/10
Posts: 18,675
Loc: Norvegr
Last seen: 18 minutes, 16 seconds
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Re: Psilocybe silvatica [Re: nomadbrad]
#19397306 - 01/10/14 04:45 AM (10 years, 1 month ago) |
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Quote:
nomadbrad said: Did you have a species in mind? I can't seem to find one that resembles it yet.
H. olivaceotinctum might be a suggestion, considering the habitat.
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Edited by Anglerfish (01/10/14 08:52 AM)
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nomadbrad
Oregrownian



Registered: 10/23/06
Posts: 1,160
Loc: Pacific NW
Last seen: 5 years, 4 months
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Quote:
Anglerfish said: H. olivaceotinQuote:
nomadbrad said: Did you have a species in mind? I can't seem to find one that resembles it yet.
H. olivaceotinctum might be a suggestion, considering the habitat.
The habitat was a bog that drains every year and it's surrounded by a diverse hardwood forest consisting of mostly maples, cotton woods, alders and oaks. The few conifers that are there are dying or already dead.
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Anglerfish
hearing things



Registered: 09/08/10
Posts: 18,675
Loc: Norvegr
Last seen: 18 minutes, 16 seconds
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Re: Psilocybe silvatic [Re: nomadbrad]
#19397926 - 01/10/14 09:02 AM (10 years, 1 month ago) |
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Quote:
nomadbrad said: The habitat was a bog
That kind of heightens the chance for one of the smaller Hypholoma species, I guess.
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nomadbrad
Oregrownian



Registered: 10/23/06
Posts: 1,160
Loc: Pacific NW
Last seen: 5 years, 4 months
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yeah it's very possible. it's time, I need a scope haha
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,752
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Re: Psilocybe silvatica [Re: nomadbrad]
#19399517 - 01/10/14 02:47 PM (10 years, 1 month ago) |
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Quote:
nomadbrad said: Did you have a species in mind? I can't seem to find one that resembles it yet.
No, I don't know the Hypholoma in that part of the world, it's best to determine genus first then look at the species known from that area.
Look for chrysocystidia in the hymenium and a subcellular layer below the pileipellis, if these are present then Hypholoma is likely.
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: Psilocybe silvatica [Re: inski]
#19400365 - 01/10/14 05:33 PM (10 years, 1 month ago) |
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I can't tell if this is a spore or not...

Can I temporarily substitute food coloring for stain?
Edited by maynardjameskeenan (01/10/14 05:36 PM)
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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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The somewhat circular, brown objects are spores. Some of them appear crushed in the above pic.
When viewing brown colored spores, you should be able to get away with just using water. Try this: Take a single gill (nothing more) - and place it on a slide. Add two drops of water. Let it sit for a few seconds then grab some tweezers and remove the gill from the slide entirely. Hopefully some spores will still be on the slide. Add one more drop of water before placing a cover slip onto the area where the gill was.
Then view for spores.
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Byrain

Registered: 01/07/10
Posts: 9,664
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Stains are entirely optional for standard microscopy, they become necessary only when trying to view obscure features like the spore nuclei in Entomophthora species or maybe to get good pictures of tiny hyaline Clitocybe spores... How are you hydrating your slides? Your section looks kind of thick, make it thinner, as thin as you can make it and then some. Small sections are easier to make thin than big ones, it takes time to perfect so try not to get discouraged.
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 6 months
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Re: Psilocybe silvatica [Re: Byrain]
#19401921 - 01/11/14 12:40 AM (10 years, 1 month ago) |
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Funny this summer I worked a 2 month contract with this lady, who during the year teaches entry biology or someshit in this college . Well after I got to know her a bit I find out she teaches microscope basics !! And shes stoked Im into mycology. So I'm like " YES this is my chance to learn this shit once and for all". So I brought my scope to work and she was going to give me a lesson but it turned out everything I subconsciously learned over the last year reading posts had actually sunk in and she was quite impressed.Because i have been really unsure if I was doing it right and I was.  I went for a quick walk at lunch, found a few specimens. Set up my scope,pulled out a fresh razor blade, cut the mushroom in half and sliced a thin slice of gill,dropped it on a fresh slide, dropped a bit of water on it and we were viewing spores in 2 minutes. She was like, what the fuck , you seem to know what your doing already!! She said I was more with it then any noob she'd taught , so thanks dudes , its all because of you. I havent been in a class room since 1997 so Ive been a bit put off by it. I need to do it more though, I still am super green. I am in the same boat as Maynard and am married with rug rats so time is hard to come by. I need to figure out how to take pics somehow and I would like to be able to measure spores.
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 6 months
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Oh and how about H marginatum.
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Quote:
psylosymonreturns said: Funny this summer I worked a 2 month contract with this lady, who during the year teaches entry biology or someshit in this college . Well after I got to know her a bit I find out she teaches microscope basics !! And shes stoked Im into mycology. So I'm like " YES this is my chance to learn this shit once and for all". So I brought my scope to work and she was going to give me a lesson but it turned out everything I subconsciously learned over the last year reading posts had actually sunk in and she was quite impressed.Because i have been really unsure if I was doing it right and I was.  I went for a quick walk at lunch, found a few specimens. Set up my scope,pulled out a fresh razor blade, cut the mushroom in half and sliced a thin slice of gill,dropped it on a fresh slide, dropped a bit of water on it and we were viewing spores in 2 minutes. She was like, what the fuck , you seem to know what your doing already!! She said I was more with it then any noob she'd taught , so thanks dudes , its all because of you. I havent been in a class room since 1997 so Ive been a bit put off by it. I need to do it more though, I still am super green. I am in the same boat as Maynard and am married with rug rats so time is hard to come by. I need to figure out how to take pics somehow and I would like to be able to measure spores.

-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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Anglerfish
hearing things



Registered: 09/08/10
Posts: 18,675
Loc: Norvegr
Last seen: 18 minutes, 16 seconds
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Quote:
psylosymonreturns said: Oh and how about H marginatum.
Stems look off, they should be silky-fibrillose. Caps should be conical to bell shaped, and with a obvious white margin.
I also never saw H. marginatum striate like that.
Although nothing is impossible, I don't think it is very likely.
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