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bootlegnjack
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Registered: 12/20/13
Posts: 4
Last seen: 2 years, 30 days
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Have reishi dowells but I want to grow in a tub or bag what would you do?
#19401979 - 01/11/14 12:56 AM (10 years, 20 days ago) |
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Hi guys,
I've bought some dowels and I've decided against logs for a number of reasons and want to try to use the dowels to grow in a a tub or bag. Here are the dowels:

Can you use dowels to colonize bags/tubs like grain spawn? I realize it might be quite a bit slower but is it still possible. One of the dramas I think will be just breaking up the tubs of dowels as they look solidified (I have two tubs). I was thinking of just plonking them in a bag of sawdust and doing a bag method or breaking them up and scattering them in a tub as if it were grain spawn. Anyone got any insight on this?
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teknix
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Registered: 09/16/08
Posts: 11,953
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: bootlegnjack]
#19402038 - 01/11/14 01:25 AM (10 years, 20 days ago) |
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Looks like they are in a petri dish, I would try to make a slant and another dish and innoc some grains with it in a glove box or under a flow hood.
But keep your dishes in a ziplock bag.
Probably wipe the dishes down with iso 91%+70% if you exposed the dishes to air, before you open them up.
If you try to transfer one to a petri dish and it shows contamination, you can dunk the next one in a 10% bleach solution to try to decontaminate the next attempt.
Edited by teknix (01/11/14 02:13 AM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: teknix]
#19402052 - 01/11/14 01:31 AM (10 years, 20 days ago) |
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70% not 91% and a sab is easier then a glove box
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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teknix
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Registered: 09/16/08
Posts: 11,953
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: cronicr]
#19402058 - 01/11/14 01:33 AM (10 years, 20 days ago) |
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Rofl, who uses 70%? Does 70% decontaminate better than 91%? sif . . .
That's just silly.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: teknix]
#19402062 - 01/11/14 01:35 AM (10 years, 20 days ago) |
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yes it does and no it's not silly, i'd explain but i'll ask you to use the search bar first;), i'm not here to attack your suggestion, just to make sure it's done right, if you can't figure it out just ask
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
Edited by cronicr (01/11/14 01:36 AM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: cronicr]
#19402063 - 01/11/14 01:37 AM (10 years, 20 days ago) |
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i'll wait while you search and make sure to leave a link
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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teknix
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π°π‘ πΌπ⨻



Registered: 09/16/08
Posts: 11,953
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: cronicr]
#19402065 - 01/11/14 01:37 AM (10 years, 20 days ago) |
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That sounds ridiculous.
Edited by teknix (01/11/14 03:36 AM)
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teknix
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Registered: 09/16/08
Posts: 11,953
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: cronicr]
#19402070 - 01/11/14 01:41 AM (10 years, 20 days ago) |
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Try google scholar, way better than the search engine at the shroomery, rofl.
http://pubs.acs.org/doi/abs/10.1021/ja00995a002 ^ Science.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: teknix]
#19402071 - 01/11/14 01:42 AM (10 years, 20 days ago) |
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Quote:
RogerRabbit said: If you're using 91% alcohol, dilute it with distilled water to give you the 60% to 70% mix you want for maximum killing power. RR
http://www.shroomery.org/forums/showflat.php/Number/14455183#14455183
It has to do with pure alcohol not being admitted inside the cell walls by osmosis. If water is mixed with the alcohol, it tricks the cell wall into admitting it, therefore killing the cell. 70% has been shown to be a good mixture for sanitizing, and superior to higher concentrations. RR
http://www.shroomery.org/forums/showflat.php/Number/10567990#10567990
You can use that if you want. Add about 30% distilled water by volume to it for best results. 91% iso alcohol is not as effective as 70%, which is what you want for sanitizing tables, gloves, tools, etc. Use the higher concentration alcohol, preferably denatured alcohol for your flame sterilization lamps. RR http://www.shroomery.org/forums/showflat.php/Number/9585095#9585095 lazy basterd i did it for ya
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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teknix
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Registered: 09/16/08
Posts: 11,953
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: cronicr]
#19402074 - 01/11/14 01:44 AM (10 years, 20 days ago) |
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hmmm...
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: teknix]
#19402075 - 01/11/14 01:45 AM (10 years, 20 days ago) |
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 op feel no need to listen to the troll here
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: cronicr]
#19402088 - 01/11/14 01:52 AM (10 years, 20 days ago) |
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oh and you will have better luck in the gourmet forum
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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teknix
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π°π‘ πΌπ⨻



Registered: 09/16/08
Posts: 11,953
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: cronicr]
#19402093 - 01/11/14 01:55 AM (10 years, 20 days ago) |
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It's actually dependent upon species as to which is most effective:
In tests of the effect of ethyl alcohol against M. tuberculosis, 95% ethanol killed the tubercle bacilli in sputum or water suspension within 15 seconds 492. In 1964, Spaulding stated that alcohols were the germicide of choice for tuberculocidal activity, and they should be the standard by which all other tuberculocides are compared. For example, he compared the tuberculocidal activity of iodophor (450 ppm), a substituted phenol (3%), and isopropanol (70%/volume) using the mucin-loop test (106 M. tuberculosis per loop) and determined the contact times needed for complete destruction were 120β180 minutes, 45β60 minutes, and 5 minutes, respectively. The mucin-loop test is a severe test developed to produce long survival times. Thus, these figures should not be extrapolated to the exposure times needed when these germicides are used on medical or surgical material 482.
http://www.cdc.gov/hicpac/disinfection_sterilization/6_0disinfection.html
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teknix
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Registered: 09/16/08
Posts: 11,953
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: teknix]
#19402100 - 01/11/14 02:02 AM (10 years, 20 days ago) |
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But yeah, You're right, 70% has been shown to kill more bacteria in some studies, I didn't know that (And I should have). thanks.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: teknix]
#19402102 - 01/11/14 02:05 AM (10 years, 20 days ago) |
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 for our hobby 70 over 91
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bootlegnjack
Stranger
Registered: 12/20/13
Posts: 4
Last seen: 2 years, 30 days
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Re: Have reishi dowells but I want to grow in a tub or bag what would you do? [Re: cronicr]
#19402160 - 01/11/14 02:49 AM (10 years, 20 days ago) |
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OK interesting discussion thanks... I'll go the 70-90% if I ever need to do that.. live and learn! 
What I'm thinking though is to see if I can put some dowels in some pasteurized oak sawdust or pellets like you would do with a G2G and either in bags or jars colonize and fruit them in a tub with verm on the bottom.
Is this crazy noob business do you think or has it got a shot of working if I keep it clean as I can?
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