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OfflineJw6457
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How long to wait for air to settle in sab b4 going to work * 1
    #19401495 - 01/10/14 10:40 PM (10 years, 1 month ago)

Just wondering how long you guys wait to go to work in your sab after loading it up with supplies and tools? I usually wait 20 minutes but just wondering how long others wait as im not sure if 20 minutes is overkill or not long enough


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Offlinesuniced
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Re: How long to wait for air to settle in sab b4 going to work [Re: Jw6457]
    #19401548 - 01/10/14 10:55 PM (10 years, 1 month ago)

i wait like 10 minutes


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Offlinedodgem
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Re: How long to wait for air to settle in sab b4 going to work [Re: Jw6457]
    #19401549 - 01/10/14 10:55 PM (10 years, 1 month ago)

I probably wait closer to 10 minutes, if that. I feel like if you are in a small enough room with no air flow 5 minutes would be enough.

But if 20 has worked for you in the past, no need to change it and risk it.


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OfflineForresterM
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Re: How long to wait for air to settle in sab b4 going to work [Re: dodgem]
    #19401623 - 01/10/14 11:21 PM (10 years, 1 month ago)

I read 15 minutes, so I tried that, and had a shitload of problems.  My house is ridden with green mold spores.  15 minutes is not near long enough. 

I wait an hour now and haven't had problems.  Terry did a post in advanced mycology about spore fall time, and some spores can take a LOOOOOOONG time to fall.  Wait an hour just to be safe :thumbup:


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-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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InvisibleFrankHorrigan
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Re: How long to wait for air to settle in sab b4 going to work [Re: Forrester] * 1
    #19401662 - 01/10/14 11:32 PM (10 years, 1 month ago)

I wait about 30 seconds.

I put my tools inside, put on my sleeves and gloves, wipe everything down with 70% iso and go to work.

I flame my scalpel and needles outside my box. When I move them inside, I count to five and carry on.

If you work in a relatively draft-free room when working in your SAB, you don't need to worry about waiting ten minutes for the air to settle. It takes much less time.


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OfflineHappy Littletree
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Re: How long to wait for air to settle in sab b4 going to work [Re: Forrester]
    #19401698 - 01/10/14 11:38 PM (10 years, 1 month ago)

Yeah I have an abundance of mold spores where I'm at too. Could you elaborate on your method for inoculation in these conditions.. do you fire outside the box.. is it a glove or just sab.. any other details would be great..


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OfflineForresterM
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Re: How long to wait for air to settle in sab b4 going to work [Re: Happy Littletree]
    #19401722 - 01/10/14 11:44 PM (10 years, 1 month ago)

Quote:

Happy Littletree said:
Yeah I have an abundance of mold spores where I'm at too. Could you elaborate on your method for inoculation in these conditions.. do you fire outside the box.. is it a glove or just sab.. any other details would be great..





I use a new scalpel blade for every culture, most of the time.  Maybe that's overkill.  If flaming a scalpel, yeah you should do it outside the box.  If flaming for syringe inoculation, I do it in open air, and don't take the foil off the tops of my jars until RIGHT before I do it, so I know they're sterile. 
Mine is just a regular SAB:



Here's the quote from Terry M about spore fall time.  (from this thread)  Terry knows his stuff.

Quote:

Terry M said:
A 2.5 micron particle takes about 44 minutes to settle. This provides a rough guide for how long you should let the air in your glove box settle!




--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineRogerRabbitM
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Re: How long to wait for air to settle in sab b4 going to work [Re: Forrester]
    #19402443 - 01/11/14 05:25 AM (10 years, 1 month ago)

Quote:

Forrester said:

and don't take the foil off the tops of my jars until RIGHT before I do it, so I know they're sterile. 





You're supposed to remove the foil as soon as you remove the jars from the sterilizer so the verm layer can dry back out.  Foil doesn't keep anything sterile.  It serves only one purpose and that's to keep the water that drips from the lid of the steam pot from getting in the jars.

To the original poster, spray the still-air-box inside with water and then use.  You don't need to wait for spore settling time.  The water you spray will knock the contaminants to the walls or floor of the box and hold them there.  Keep your flame on the outside of the box.  Pull your arm out to flame the tools and then re-insert and work.

Still air boxes are not sterile, nor is the air inside them sterile.  It's only a way to stop drafts while you work, quickly.
RR


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OfflineForresterM
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Re: How long to wait for air to settle in sab b4 going to work [Re: RogerRabbit]
    #19402595 - 01/11/14 07:05 AM (10 years, 1 month ago)

Quote:

RogerRabbit said:
You're supposed to remove the foil as soon as you remove the jars from the sterilizer so the verm layer can dry back out.  Foil doesn't keep anything sterile.  It serves only one purpose and that's to keep the water that drips from the lid of the steam pot from getting in the jars.





I wasn't talking Pf cakes, I leave foil on grain jars so the injection port is clean when I inject into it.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinedodgem
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Re: How long to wait for air to settle in sab b4 going to work [Re: Forrester]
    #19402717 - 01/11/14 08:06 AM (10 years, 1 month ago)

Reasoning behind keeping the flame out side of the box? I have always flamed inside when doing Agar.


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Invisibleelasticaltiger
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Re: How long to wait for air to settle in sab b4 going to work [Re: dodgem]
    #19402723 - 01/11/14 08:10 AM (10 years, 1 month ago)

Quote:

dodgem said:
Reasoning behind keeping the flame out side of the box? I have always flamed inside when doing Agar.




Alcohol fumes on your gloves can ignite.  It's just better over all to have that flame out in the open.


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OfflineCMOS
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Re: How long to wait for air to settle in sab b4 going to work [Re: elasticaltiger]
    #19402729 - 01/11/14 08:14 AM (10 years, 1 month ago)

Quote:

elasticaltiger said:
Quote:

dodgem said:
Reasoning behind keeping the flame out side of the box? I have always flamed inside when doing Agar.




Alcohol fumes on your gloves can ignite.  It's just better over all to have that flame out in the open.




and it stirs up the air in the glovebox


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OfflineJw6457
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Re: How long to wait for air to settle in sab b4 going to work [Re: CMOS]
    #19403064 - 01/11/14 10:17 AM (10 years, 1 month ago)

Ok thanks guys. I do all my flaming outside the box. I wipe the walls and bottom of sab with soapy water and put soapy water soaked paper towels on the bottom of my sab. Wipe all my stuff with iso put in sab, put lid on and wait 20 minutes sitting in a sealed up bathroom with painters plastic sheeting wrapped around the door in order to completely seal the bathroom from drafts.

So i sit down and wait 20 minutes breathing in soapy water that i spray everywhere inside the bathroom so shit sticks to that too. I dont mind waiting 20 minutes but when i have to do 2 loads of jars with g2g cause they dont all fit in my sab, i end up in the bathroom for 2-2.5 hours cause of wait and prep time of sab.

Ill go with 10 min from now on


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OfflineSillyputty67

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Re: How long to wait for air to settle in sab b4 going to work *DELETED* [Re: Jw6457]
    #19403440 - 01/11/14 11:46 AM (10 years, 1 month ago)

Post deleted by Sillyputty67

Reason for deletion: 1



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1) Everything I ever posted or say is a lie.


Edited by Sillyputty67 (01/11/14 11:55 AM)


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OfflineJw6457
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Re: How long to wait for air to settle in sab b4 going to work [Re: Sillyputty67]
    #19405063 - 01/11/14 06:11 PM (10 years, 1 month ago)

Im just talking about how long to wait to enter my sab man. I mainly do g2g now and am doing agar and use an exacto knife so i sterilize it in a jar and flame between each culture or grain jar. Works as well as a scalpel. Thanks for your input though


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Offlinealabamamushie
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Re: How long to wait for air to settle in sab b4 going to work [Re: Jw6457]
    #19405110 - 01/11/14 06:19 PM (10 years, 1 month ago)

1 hour is good for everything to settle inside once you loaded it


:thumbup:


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Offlinetwistedty
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Re: How long to wait for air to settle in sab b4 going to work [Re: FrankHorrigan]
    #19405119 - 01/11/14 06:20 PM (10 years, 1 month ago)

Quote:

FrankHorrigan said:
I wait about 30 seconds.

I put my tools inside, put on my sleeves and gloves, wipe everything down with 70% iso and go to work.

I flame my scalpel and needles outside my box. When I move them inside, I count to five and carry on.

If you work in a relatively draft-free room when working in your SAB, you don't need to worry about waiting ten minutes for the air to settle. It takes much less time.




aye me too, i just spray er down with isoproply and then follow it with water and do work the SAB itself not including tools dishes or w/e im working with


Edited by twistedty (01/11/14 06:20 PM)


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