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OfflineJw6457
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Agar wedges stuck on glass and lid problem?
    #19400809 - 01/10/14 07:36 PM (10 years, 1 month ago)

Hey guys new with agar did my 2nd transfer of agar to grains but this time i didnt realize your not suppose to shake agar and grains like you would g2g because it gets stuck.

I got a few wedges stuck on the glass above the grains and some on the actual lid am i screwed with those jars?


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OfflinecronicrFacebook
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Re: Agar wedges stuck on glass and lid problem? [Re: Jw6457]
    #19400817 - 01/10/14 07:38 PM (10 years, 1 month ago)

your fucked....lol i'm just kidding i just shook the fuck out of one at whippy request, worked out fine. maybe just put your jar upside down or on it's side for now:shrug:


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OfflineCMOS
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Re: Agar wedges stuck on glass and lid problem? [Re: cronicr]
    #19400822 - 01/10/14 07:41 PM (10 years, 1 month ago)

Yeah I had a jar that no amount of shaking would dislodge the agar wedge from the lid.  Tried for like 5min.  Just turned it upside down till it colonized some grains, then reshook later.

I still like shaking agar inoculated jar, I try and get the wedge right in the middle of the grain jar.  Normally it ends up stuck to the side though.....


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InvisibleEnigma1
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Re: Agar wedges stuck on glass and lid problem? [Re: Jw6457]
    #19400849 - 01/10/14 07:45 PM (10 years, 1 month ago)

Use a glove box and take them off the glass.


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InvisiblebodhisattaMDiscordReddit
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Re: Agar wedges stuck on glass and lid problem? [Re: cronicr]
    #19400880 - 01/10/14 07:53 PM (10 years, 1 month ago)

Quote:

cronicr said:
your fucked....lol i'm just kidding i just shook the fuck out of one at whippy request, worked out fine. maybe just put your jar upside down or on it's side for now:shrug:




yea, RR dogma is not to shake but let the agar grow off as recovery and to see any contams. But if it contams it contams anyway :shrug: so if it's clean doesn't the shake help.


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OfflineJw6457
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Re: Agar wedges stuck on glass and lid problem? [Re: bodhisatta]
    #19400922 - 01/10/14 08:02 PM (10 years, 1 month ago)

I have like 5-10 small wedges in each master and i think only 1 jar has 2 wedges jammed up against the lid. I literally shook the shit out of the jar so much the shells from the grains are all over the sides of the jar. Ill leave it as is and hope its just like any other grains that get stuck up on the lid. They colonize fine and when i spawn i just scrape the shit off the lid into the trash. Thanks a bunch guys. I think im going to start using large wedges rather then a bunch of small ones


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InvisiblebodhisattaMDiscordReddit
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Re: Agar wedges stuck on glass and lid problem? [Re: Jw6457]
    #19401027 - 01/10/14 08:31 PM (10 years, 1 month ago)

Quote:

Jw6457 said:
I have like 5-10 small wedges in each master and i think only 1 jar has 2 wedges jammed up against the lid. I literally shook the shit out of the jar so much the shells from the grains are all over the sides of the jar. Ill leave it as is and hope its just like any other grains that get stuck up on the lid. They colonize fine and when i spawn i just scrape the shit off the lid into the trash. Thanks a bunch guys. I think im going to start using large wedges rather then a bunch of small ones




each wedge you put in a jar is a chance for a contam, I would do one quick and swift wedge into the jar.


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