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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Time will tell but its working....
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 6 months, 14 days
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Re: Another first time grow [Re: cronicr]
#19307519 - 12/21/13 11:28 AM (10 years, 2 months ago) |
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15cc is definitely a little excessive. It could possibly ruin the moisture content. If the LC in the syringe was mostly solid mycelium and very little liquid you can get away with using a fair bit. You still wouldn't usually go above a cc or 2 per injection point, but it seems to be working so far. I'd replace that tape with micropore tape and get your gas exchange moving a bit.
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Re: Another first time grow [Re: vikingsc]
#19307662 - 12/21/13 11:54 AM (10 years, 2 months ago) |
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Will do, thank you guys! Its amazing how much you can try to prepare, and still mess up so many little things. I will keep you everything updated with progress over the next few weeks.
Is it bad manners to only post on my own stuff at first? I feel like I should have some experience before commenting on others' work...
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chowster
Happy Farmer

Registered: 11/20/13
Posts: 182
Last seen: 2 years, 11 months
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Or does it colonize retarded fast?
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 6 months, 14 days
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nahh, it's all good.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Quote:
kaptanoblivious said: Will do, thank you guys! Its amazing how much you can try to prepare, and still mess up so many little things. I will keep you everything updated with progress over the next few weeks.
Is it bad manners to only post on my own stuff at first? I feel like I should have some experience before commenting on others' work...
No comment away its a great way to learn, worse comes to worse n u say something wrong someone will correct u just dont take it personelly
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 6 months, 14 days
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Re: Another first time grow [Re: cronicr]
#19308341 - 12/21/13 02:53 PM (10 years, 2 months ago) |
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I like Chowster's avatar. That's a cool pic
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Re: Another first time grow [Re: vikingsc]
#19308454 - 12/21/13 03:31 PM (10 years, 2 months ago) |
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So I found the cloth tape that I used, and it is heavy duty and "breathable". I think that's why I thought: meh, close enough, when I originally started. I think the risk of replacing it isn't worth possibly contaminating everything, so I will keep it as is, unless the jars stall.
and yeah chowster's avatar is awesome, what is it from?
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 6 months, 14 days
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If you have a dry verm layer, which it looks like you do, it's not much of a risk to take the tape off and replace. Lot's of people don't even use micropore tape and only depend on the dry verm layer as a filter for airborne contams.
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 6 months, 14 days
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Re: Another first time grow [Re: vikingsc]
#19308500 - 12/21/13 03:45 PM (10 years, 2 months ago) |
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From the pic it just looks like it might be a little bit too heavy duty and restrict gas exchange more than micropore tape would. But of course it's up to you.
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Re: Another first time grow [Re: vikingsc]
#19312085 - 12/22/13 02:19 PM (10 years, 2 months ago) |
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Okay time for a mini update, this is Day 6 since I injected the LC.
I took the advice and bought some micropore tape and replaced everything. It was a good thing I did because the tape I had on there seemed hardened by the pressure cooking, and didn't seem like it was letting any air through. I went to three different stores before I realized I had been staring at micropore tape at least once before...

So I had a question about some of my jars... 2-3 of them seem to be not doing as well as the others. I posted a comparison of a "good" jar below one of the "bad" ones. Bonus micropore tape.

Closeup of the "bad" jar:
 
Closeup of the "good" jar:

So it seems like in these "bad" jars that parts of the mycellium is more of a washed out gray color due to much more sparse growth. It started out similar to this in my other jars, before becoming brighter white and looking healthy, but these 2-3 jars seem to be stuck like this. I'm not sure if some sort of cobweb-type mold contamination or just poor growth due to little gas escape. It's hard to take good pics on a bad camera, but I can see some strandy, rhizomorphic-like growth in these bad areas. All were from one LC, so if there is contamination I would expect it to be uniform.
On another note, my weird quinoa mix jars are doing just dandy so far

all in all not too bad, with 9-10 1 pint jars doing well, and 2-3 not so well. My two bigger ~quart quinoa mix jars are also doing quite well.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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mycelium has a hard time colonizing saturated substrates...
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Re: Another first time grow [Re: cronicr]
#19312180 - 12/22/13 02:44 PM (10 years, 2 months ago) |
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Yeah I guess I erred on the wet side of things when I was setting up the jars, then I dumped a crapload of LC into each jar. Is there anything I could do at this point? I could throw some of the jars somewhere with very low humidity, if that'll do anything.
Thank you again for the rapid advice cronicr.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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just wait it out, it may have a hard time and take a little longer but there's not much you can do about it at this point
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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crackacola
Big Daddy


Registered: 12/14/13
Posts: 234
Last seen: 8 years, 10 months
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Re: Another first time grow [Re: cronicr]
#19313226 - 12/22/13 07:04 PM (10 years, 2 months ago) |
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haha. I'm laughing so hard at the 15cc per jar. I was hoping that was a typo. Those things are loooooged. lol. If you do end up with a lot of free standing water on the bottom of those jars, flip them over. It'll work its way into the bottom of the dry verm and then DO NOT flip them back over until they're fully colonized. That's about the only time you should ever flip your jars over. I'm honestly surprised they're doing as well as they are.
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Re: Another first time grow [Re: crackacola]
#19314042 - 12/22/13 10:43 PM (10 years, 2 months ago) |
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well on the plus side maybe I don't need to dunk them now?
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Well the straggling jars are catching up and its day 8 with ~50-80% colonization in the 12 brf jars, and the mycelium is very aggressively invading the 'dry' verm layer... Much more aggressively than in the rest of the jar... I'd say the moisture problem is real, ha. Progress is likely a result of the huge amount of LC used. Everything has been sitting by a heater vent which fluctuates between 67-80F. And there is no pooling at the bottom of any jars... I didn't eff them up that bad 
No contams yet
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Edited by kaptanoblivious (12/26/13 01:47 PM)
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crackacola
Big Daddy


Registered: 12/14/13
Posts: 234
Last seen: 8 years, 10 months
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Sounds like you'll be ok. Keep your fingers crossed that the excess water in the dry verm layer doesn't pick up any contams. Inspect closely when you birth them.
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FOR TRADE <---- Check out my spores for trade!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Good shit
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Re: Another first time grow [Re: cronicr]
#19400846 - 01/10/14 07:45 PM (10 years, 1 month ago) |
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Another update
My agar work has been going great, and I have 4 clean isolates from my spore syringes for each Z strain and Cambodian. I also have a clean multispore LC for both of these. I got some nice quart jars and WBS so I will be switching to this method for future endeavors.
On to the main point.. my BRF pint jars were pretty slow going after the initial burst of growth from the LC, but they were fully colonized about a week ago. Today I birthed them and started the dunk before I use these as spawn for coir/verm. I was worried I wouldn't know what a mushroom smell meant... but they all smelled wonderfully like store bought mushrooms.
Out of curiosity I broke one open, and the core was not colonized... So I broke them all open and the same thing for almost all of them. I washed out the uncolonized BRF substrate and now am dunking all of my halves.
Is there anything that can be done to minimize contamination issues at this point? There is a bunch of junk floating in the water so I might change the dunking water soon.
I am constructing my monotub tonight so I will have that to keep me busy at least. Sorry for no pictures at this point, I will update with pictures when there is something more exciting going on.
Edit: I finished the Damion5050 tek without too many problems. I spawned all 12 BRF pint jars to one monotub using the tek recipe. The only way I deviated is by warming up the bucket and coir/verm to ~150F before dumping the boiling water in. Once this either looks fully colonized or contaminates or whatever, I will update with some pictures. I'm hoping to get one flush out of this.
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Edited by kaptanoblivious (01/11/14 04:27 PM)
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