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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
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Noob wants to clone
#19399325 - 01/10/14 02:03 PM (10 years, 20 days ago) |
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Hey, I've got some cakes doing some awesome things right now, and I would like to have a first successful attempt at cloning them. I have tried to spore print twice with no success. I WOULD try again, accept these drop no spores! Nothing to collect... It seems like cloning using living tissue would be a relatively easy transition; if someone had any up to date TEK/ ideas on that, I'd be stoked. I have made 1 or 2 successful LC's using MS, but it seems people are not into LC on this site. Agar seems cool, but out of my price range for purchasing supplies/equipment at this time. Would transferring fresh fruit to sterile grain be an easy learning curve? I have only ever really been successful with cakes, though have gotten WBS and corn to colonize and fruit in vitro. Thanks.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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you would learn that trich is a bitch real fast lol! agar isn't that expensive, you could search the brf agar substitute for a starting point
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 2 months
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Re: Noob wants to clone [Re: cronicr]
#19399356 - 01/10/14 02:10 PM (10 years, 20 days ago) |
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http://www.shroomery.org/8514/Agar-substitute
search no more. Works great. I"m doing it now
Don't count LC out man...you should try every possible way of cultivation that strikes your interest. We're all different, and you may love LC. I did a half ass job on my LC, just to try it, and it turned out great so far.
Do it all man, pick what you love and master that shit.
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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NecroMyce
Mostly Ghostly



Registered: 05/12/13
Posts: 747
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Re: Noob wants to clone [Re: cronicr]
#19399362 - 01/10/14 02:10 PM (10 years, 20 days ago) |
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http://www.shroomery.org/forums/showflat.php/Number/19385884 heres my baby steps into agar, check this over and look at the links in the beginning OP
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 2 months
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Re: Noob wants to clone [Re: NecroMyce]
#19399384 - 01/10/14 02:15 PM (10 years, 20 days ago) |
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if youre gonna try it, use jars with clear glass all arond, or some pp5 plastic so you can see better
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
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Re: Noob wants to clone [Re: NecroMyce]
#19399394 - 01/10/14 02:17 PM (10 years, 20 days ago) |
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Thanks guys. I don't fuck with trich cronicr, so are you saying living tissue transfer is shitty? Yeah I dig what you're saying about trying everything, 1down5up. If I can find some squat jars, I got the syrup, fuckin a, new LC attempt! You guys left some good links, I'ma read up, thanks!
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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
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1up5down...conversion of brown rice to already ground BRF? I have Bobs Red mill BRF...
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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yeah tissue to grain usually doesn't go good at all bob mills works great
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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NecroMyce
Mostly Ghostly



Registered: 05/12/13
Posts: 747
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He is saying, if im correct. That if you wish to freshly pick a fruit. then cut off a wedge from the stipe and drop it directly into a Rye/WBS grain jar, or any jar to colonize for that matter, ive read that you are about 80% of chance to get trich/contams.
you want to transfer to agar plates and then do a few sector transfers to create stronger/specifc/individual growth, and ensure a "clean" isolate. And by clean i mean most spore syringes and shit can have trich or anytype of bacteria dormant inside its beautiful liquids :/ So when you do some transfers to get singled out isolates. Its guarnteed clean colonization unless another outside source that straight fucks your jars at night sideways while pulling their hair and calling them sally, then leaves during the day. Not only to see the damage in the morning and cry yourself back to sleep. aka trichoderma...
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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
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Re: Noob wants to clone [Re: NecroMyce]
#19399470 - 01/10/14 02:40 PM (10 years, 20 days ago) |
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Well Caspuuur I don't like those odds at all! So when I get MS syringes from a vendor, utilize sterile technique to a T, using the same methods and variables that have always granted me success; and then get ten jars that smell like cottage cheese within days of inoculation and don't germinate or show trich or green penicilin within the 7-10 day window, IT COULD be the vendors fault??!! There are nasty things in his MS syringes that I'm paying 12-20 bucks for??!! Fuck that, now I'm just mad! GRRRRRR!!!
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 2 months
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Yes, sometimes there are nasties...but if you get one decent mush out of it, you print and clone the fucker. Never need another vendor again.
I used 3.5 g of honey, and 96g of water, swirled it around, PC'd it, and when it cooled, i red hot fucked a sterilized syringe, stuck it through the stem of a sweet ass fruit, (after swabbing the entire area with alcohol wipe), and shot the little tiny piece from the syringe into the honey LC. All done in a SAB. SOme time later i got a good blob of myc. Shook it up, and sucked up some of it with a syringe i PC'd. Aces. ONe drop on the BRF sub and myc is growing great. Again, all done in a SAB.
I use Bobs BRF and its working for me...even used it for my agar substitute.

^^that's a cloned piece of my first fruit, from my first cake on my first grow.
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
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Re: Noob wants to clone [Re: 1down5up]
#19399601 - 01/10/14 03:05 PM (10 years, 20 days ago) |
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This looks awesome Bro, I can't wait to try! Fuck waiting for vendor to replace, I'm cleaning house tonight! PC no more than 15-20 minutes, yeah? Maybe PM you later so we can stop bumping this and pissing mods off?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Agar is cheap if you can find it at a asian grocery store. I just bought 5 25g packets today for $6 with tax. That's 125g of agar enough to make a little over 5 liters or ~1.2 gallons of agar.
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 2 months
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I don't thinks the mods mind if it's constructive man...after all, that's why we're here. Just use the search function, and do a little reading. If you have any questions, post them in this thread. Most of the people that pop in to help will follow along, and guide you as best they can. You'll be running along in no time
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 14 hours, 49 minutes
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Quote:
bodhisatta said: Agar is cheap if you can find it at a asian grocery store. I just bought 5 25g packets today for $6 with tax. That's 125g of agar enough to make a little over 5 liters or ~1.2 gallons of agar.
You can get it fairly cheap online too. Looking on Ebay right now there's a 500g bag for about $30.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Noob wants to clone [Re: Kizzle]
#19399711 - 01/10/14 03:35 PM (10 years, 20 days ago) |
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i paid about 100 bux for 2 lbs i think
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
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Re: Noob wants to clone [Re: cronicr]
#19399762 - 01/10/14 03:44 PM (10 years, 20 days ago) |
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Right on guys...seems cheap, I was always under the impression that you needed other organic mediums too, like dextrose, malt extract, etc. I see Teks for plates using more than one store bought ingredient, so I wonder. And could I get plates/slants in town anywhere or just online? Or use half pint jars or similar like 1down5up does for his BRF goo?
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magickspore



Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 8 months, 4 days
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Yea I get a lb of agar for 40 (my local health food store orders it for me). ANYTHING PP5 or PCable!! GLAD MINI ROUNDS!! W.E. YOU CAN FIND!!
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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
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PP5? plastic grading I'm guessing, must be safe enough to PC...I have glad tupperware that is round! ooooh here we go!
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magickspore



Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 8 months, 4 days
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No PP stands for pasty plates... No PP is polypropylene. just look on the bottom should say PP5.
Edited by magickspore (01/10/14 04:37 PM)
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livingmagic
ã‚‹ã‚“ã‚‹ã‚“



Registered: 12/03/13
Posts: 3,015
Loc: Big Green Amore
Last seen: 2 days, 3 hours
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NecroMyce
Mostly Ghostly



Registered: 05/12/13
Posts: 747
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I've stopped doing 1/2 pint BRF recently. So i just made up a bunch of new lids for my agar. and used my half pint jars.
http://www.shroomery.org/forums/showflat.php/Number/16590981/fpart/1/vc/1
instead of
http://www.mushroomvideos.com/Agar-Petri-Dishes
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 23 days
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Re: Noob wants to clone [Re: NecroMyce]
#19407538 - 01/12/14 09:41 AM (10 years, 19 days ago) |
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Just thought I'd chime in here. I do clones from fruit bodies by growing a few fruits invitro and pulling and dropping them into new sterilized grain jars with sterilized tweezers. works great, increases yeild, and still provides a little genetic variation to spice up my monos. Its a little more involved than g2g but I havent had a single jar fail in months, and thats about 4 dozen jars every two weeks spawned out with no probs.
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
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Re: Noob wants to clone [Re: Zenlykos]
#19408545 - 01/12/14 02:12 PM (10 years, 18 days ago) |
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I love this site. One person tells me I will have an 80% rate of failure doing transfers with fruit bodies, and someone else tells me that they've had nothing but success recently. Awesomely confused.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Noob wants to clone [Re: Zenlykos]
#19408553 - 01/12/14 02:14 PM (10 years, 18 days ago) |
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Quote:
Zenlykos said: Just thought I'd chime in here. I do clones from fruit bodies by growing a few fruits invitro and pulling and dropping them into new sterilized grain jars with sterilized tweezers. works great, increases yeild, and still provides a little genetic variation to spice up my monos. Its a little more involved than g2g but I havent had a single jar fail in months, and thats about 4 dozen jars every two weeks spawned out with no probs.
pics? grow log?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Noob wants to clone [Re: cronicr]
#19408558 - 01/12/14 02:16 PM (10 years, 18 days ago) |
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unless your growing invitro like him and have a LFH i wouldn't bother
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
Edited by cronicr (01/12/14 02:56 PM)
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 2 months
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Re: Noob wants to clone [Re: cronicr]
#19408690 - 01/12/14 02:54 PM (10 years, 18 days ago) |
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If you do transfers with in vitro fruit bodies, wouldn't you not know what other traits the fruit has until you see it grow out a little? You've got colonization speed, and fruiting speed, but no indication of anything afterwards. Would it not make more sense to take a fruit body which has had some time to grow out a little, with desirable traits, and then clone to agar/agar substitute? Not a mature fruit, which is at the end of it's cycle, but somewhere inbetween? Just curious, i think this Q? also applies to the questions raised in this thread....
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 23 days
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Re: Noob wants to clone [Re: 1down5up]
#19418280 - 01/14/14 04:57 PM (10 years, 16 days ago) |
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Quote:
1down5up said: If you do transfers with in vitro fruit bodies, wouldn't you not know what other traits the fruit has until you see it grow out a little? You've got colonization speed, and fruiting speed, but no indication of anything afterwards. Would it not make more sense to take a fruit body which has had some time to grow out a little, with desirable traits, and then clone to agar/agar substitute? Not a mature fruit, which is at the end of it's cycle, but somewhere inbetween? Just curious, i think this Q? also applies to the questions raised in this thread....
I usually wait until the invitro fruits have started to barely show the veil pulling away from the stem before I use them to clone. That way I am able to check for any visible defects. #1 reason why I do it this way though is to allow myself a surprise from tub to tub. As i clone out from generation to generation, there is less variation, and I'm able to (I say this loosely) Isolate the characteristics I find most appealing. It takes up more space and time than agar work, but allows me to always have enough work on hand to satisfy my impatience, and keeps crops moving at a reasonable pace.
Also worth noting: I don't use the caps of the fruits when i clone, just the midsection. I don't have any solid scientific reason for it, but that's the way I do it.
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 23 days
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Re: Noob wants to clone [Re: cronicr]
#19418314 - 01/14/14 05:04 PM (10 years, 16 days ago) |
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Quote:
cronicr said:
Quote:
Zenlykos said: Just thought I'd chime in here. I do clones from fruit bodies by growing a few fruits invitro and pulling and dropping them into new sterilized grain jars with sterilized tweezers. works great, increases yeild, and still provides a little genetic variation to spice up my monos. Its a little more involved than g2g but I havent had a single jar fail in months, and thats about 4 dozen jars every two weeks spawned out with no probs.
pics? grow log?
I'll see about posting some pics and logs soon. But the general idea is 16 monos, 6/10 split. as one set begins fruiting, another set is spawned. 5q-5q spawn ratio, with excess jars running g2g for carryover, and 5-7 jars with clone/clone generations. 1:1:1 coir/manure/humus&manure fertilizer
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Noob wants to clone [Re: Zenlykos]
#19418322 - 01/14/14 05:05 PM (10 years, 16 days ago) |
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for sure you should doc something like that
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Zenlykos
Wandering One



Registered: 12/25/12
Posts: 234
Last seen: 1 year, 23 days
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Re: Noob wants to clone [Re: cronicr]
#19418374 - 01/14/14 05:14 PM (10 years, 16 days ago) |
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Will do. It's been a while since I've logged anything on here do to the large amount of illegality of my current grow, and spending the time to learn from experience what was holding my grows back instead of running on here for answers every five seconds lol
-------------------- I worship the tree that is not there. To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit. Anything I post is purely fictional and is not to be taken seriously.
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