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Offlinelivingmagic
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Registered: 12/03/13
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Re: Noob wants to clone [Re: Bucknaztee]
    #19400022 - 01/10/14 04:28 PM (10 years, 20 days ago)



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InvisibleNecroMyce
Mostly Ghostly
I'm a teapot


Registered: 05/12/13
Posts: 747
Re: Noob wants to clone [Re: livingmagic]
    #19407405 - 01/12/14 08:57 AM (10 years, 19 days ago)

I've stopped doing 1/2 pint BRF recently. So i just made up a bunch of new lids for my agar. and used my half pint jars.

http://www.shroomery.org/forums/showflat.php/Number/16590981/fpart/1/vc/1

instead of

http://www.mushroomvideos.com/Agar-Petri-Dishes


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OfflineZenlykos
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Re: Noob wants to clone [Re: NecroMyce]
    #19407538 - 01/12/14 09:41 AM (10 years, 19 days ago)

Just thought I'd chime in here. I do clones from fruit bodies by growing a few fruits invitro and pulling and dropping them into new sterilized grain jars with sterilized tweezers. works great, increases yeild, and still provides a little genetic variation to spice up my monos. Its a little more involved than g2g but I havent had a single jar fail in months, and thats about 4 dozen jars every two weeks spawned out with no probs.


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I worship the tree that is not there.

To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit.

Anything I post is purely fictional and is not to be taken seriously. :mushroom2:


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OfflineBucknaztee
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Registered: 11/21/09
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Re: Noob wants to clone [Re: Zenlykos]
    #19408545 - 01/12/14 02:12 PM (10 years, 18 days ago)

I love this site. One person tells me I will have an 80% rate of failure doing transfers with fruit bodies, and someone else tells me that they've had nothing but success recently. Awesomely confused.


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OfflinecronicrFacebook
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Re: Noob wants to clone [Re: Zenlykos]
    #19408553 - 01/12/14 02:14 PM (10 years, 18 days ago)

Quote:

Zenlykos said:
Just thought I'd chime in here. I do clones from fruit bodies by growing a few fruits invitro and pulling and dropping them into new sterilized grain jars with sterilized tweezers. works great, increases yeild, and still provides a little genetic variation to spice up my monos. Its a little more involved than g2g but I havent had a single jar fail in months, and thats about 4 dozen jars every two weeks spawned out with no probs.



pics? grow log?


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinecronicrFacebook
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Re: Noob wants to clone [Re: cronicr]
    #19408558 - 01/12/14 02:16 PM (10 years, 18 days ago)

unless your growing invitro like him and have a LFH i wouldn't bother


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Edited by cronicr (01/12/14 02:56 PM)


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Offline1down5up
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Re: Noob wants to clone [Re: cronicr]
    #19408690 - 01/12/14 02:54 PM (10 years, 18 days ago)

If you do transfers with in vitro fruit bodies, wouldn't you not know what other traits the fruit has until you see it grow out a little?  You've got colonization speed, and fruiting speed, but no indication of anything afterwards.  Would it not make more sense to take a fruit body which has had some time to grow out a little, with desirable traits, and then clone to agar/agar substitute?  Not a mature fruit, which is at the end of it's cycle, but somewhere inbetween? Just curious, i think this Q? also applies to the questions raised in this thread....


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~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


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OfflineZenlykos
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Re: Noob wants to clone [Re: 1down5up]
    #19418280 - 01/14/14 04:57 PM (10 years, 16 days ago)

Quote:

1down5up said:
If you do transfers with in vitro fruit bodies, wouldn't you not know what other traits the fruit has until you see it grow out a little?  You've got colonization speed, and fruiting speed, but no indication of anything afterwards.  Would it not make more sense to take a fruit body which has had some time to grow out a little, with desirable traits, and then clone to agar/agar substitute?  Not a mature fruit, which is at the end of it's cycle, but somewhere inbetween? Just curious, i think this Q? also applies to the questions raised in this thread....




I usually wait until the invitro fruits have started to barely show the veil pulling away from the stem before I use them to clone. That way I am able to check for any visible defects. #1 reason why I do it this way though is to allow myself a surprise from tub to tub. As i clone out from generation to generation, there is less variation, and I'm able to (I say this loosely) Isolate the characteristics I find most appealing. It takes up more space and time than agar work, but allows me to always have enough work on hand to satisfy my impatience, and keeps crops moving at a reasonable pace.

Also worth noting: I don't use the caps of the fruits when i clone, just the midsection. I don't have any solid scientific reason for it, but that's the way I do it.


--------------------
I worship the tree that is not there.

To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit.

Anything I post is purely fictional and is not to be taken seriously. :mushroom2:


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OfflineZenlykos
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Registered: 12/25/12
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Re: Noob wants to clone [Re: cronicr]
    #19418314 - 01/14/14 05:04 PM (10 years, 16 days ago)

Quote:

cronicr said:
Quote:

Zenlykos said:
Just thought I'd chime in here. I do clones from fruit bodies by growing a few fruits invitro and pulling and dropping them into new sterilized grain jars with sterilized tweezers. works great, increases yeild, and still provides a little genetic variation to spice up my monos. Its a little more involved than g2g but I havent had a single jar fail in months, and thats about 4 dozen jars every two weeks spawned out with no probs.



pics? grow log?




I'll see about posting some pics and logs soon. But the general idea is 16 monos, 6/10 split. as one set begins fruiting, another set is spawned.
5q-5q spawn ratio, with excess jars running g2g for carryover, and 5-7 jars with clone/clone generations.
1:1:1 coir/manure/humus&manure fertilizer


--------------------
I worship the tree that is not there.

To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit.

Anything I post is purely fictional and is not to be taken seriously. :mushroom2:


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OfflinecronicrFacebook
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Re: Noob wants to clone [Re: Zenlykos]
    #19418322 - 01/14/14 05:05 PM (10 years, 16 days ago)

for sure you should doc something like that:thumbup:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineZenlykos
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Registered: 12/25/12
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Re: Noob wants to clone [Re: cronicr]
    #19418374 - 01/14/14 05:14 PM (10 years, 16 days ago)

Will do. It's been a while since I've logged anything on here do to the large amount of illegality of my current grow, and spending the time to learn from experience what was holding my grows back instead of running on here for answers every five seconds lol


--------------------
I worship the tree that is not there.

To readers: all of my posts are of knowledge I have obtained from practice, reputable sources, and common knowledge. If anything I say is incorrect, please feel free to correct me so that everyone can benefit.

Anything I post is purely fictional and is not to be taken seriously. :mushroom2:


Extras: Filter Print Post Top
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