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Offlinejoegibbs
Stranger
Registered: 12/30/12
Posts: 23
Last seen: 5 years, 5 months
Bacteria on Rye Spawn?
    #19398954 - 01/10/14 01:00 PM (10 years, 20 days ago)



I've got several jars with this.  Used a sterile glove box during inoculation.  Gloves, tyvek suit, surgical mask, the works.  Two of the jars had a tiny bit of this, but I used them anyways and they have fully colonized my h. poo/straw tubs with no sign of contams.  Is this safe to use?


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OfflineCamtaro420
Birdman
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Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 5 years, 5 months
Re: Bacteria on Rye Spawn? [Re: joegibbs]
    #19398981 - 01/10/14 01:05 PM (10 years, 20 days ago)

Quote:

joegibbs said:


I've got several jars with this.  Used a sterile glove box during inoculation.  Gloves, tyvek suit, surgical mask, the works.




How did you prepare the rye though?

was it dry enough when placed inside the jar?

was it PC'd or steamed and how long either way?

More info. I wouldn't use that, it looks gross, but it might be safe. Wait for a pro and answer the questions!


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OfflinePaulsage
Shaman of shit storms
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Registered: 09/01/12
Posts: 109
Last seen: 6 years, 5 months
Re: Bacteria on Rye Spawn? [Re: joegibbs]
    #19398992 - 01/10/14 01:07 PM (10 years, 20 days ago)

Quote:

I've got several jars with this.  Used a sterile glove box during inoculation.  Gloves, tyvek suit, surgical mask, the works.  Two of the jars had a tiny bit of this, but I used them anyways and they have fully colonized my h. poo/straw tubs with no sign of contams.  Is this safe to use?




looks like it's bubbling and percolating...  i'd get rid of it...  something is definitely going on in there IMO...


--------------------
"The brain is a reducing valve that restricts consciousness" - A Huxley

"Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson…

"Whatever you study you also change" - Heisenberg Uncertainty principle


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Bacteria on Rye Spawn? [Re: Paulsage]
    #19399007 - 01/10/14 01:11 PM (10 years, 20 days ago)

thats fucked, not even the myc is the right myc, start over and good luck


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Bacteria on Rye Spawn? [Re: Camtaro420]
    #19399008 - 01/10/14 01:11 PM (10 years, 20 days ago)

I wouldn't consider any use of that in a big tub.  I am going to take a guess that you used a MS syringe....If you were as careful as you sound, and I don't doubt it, I would imagine that it came with the syringe.  IT does happen.

If you want to study something, knowing that you will probably waste time It could be spawned to a mini-mono.  Try and remove as much of the infected area, but know you will never get it all out.  Spoon out what looks good maby?  I have done this in the past, and success is what you define it as.  You may get a few fruits, it may work great, or it may succumb to another infection quickly. 

Things like that make for great out-door beds.  Mother nature has a way of cleaning stuff like that up.


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Offlinejoegibbs
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Registered: 12/30/12
Posts: 23
Last seen: 5 years, 5 months
Re: Bacteria on Rye Spawn? [Re: Paulsage]
    #19399029 - 01/10/14 01:14 PM (10 years, 20 days ago)

Quote:

How did you prepare the rye though?

was it dry enough when placed inside the jar?

was it PC'd or steamed and how long either way?




Rye was prepared using the exact procedures from the "Let's Grow Mushrooms" series.  Rinsed and cleaned, gypsum added, soaked 18-hrs, boiled for 10 minutes, strained.  Toilet paper test used to determine wetness, though it was a bit wetter than it normally is. 

Jars loaded, inoculation port lids used.  Sterilized in PC for 2hrs.

Inoculated in sterile glove box, with needle going into alcohol between each jar. 

The spore syringe I used was a bit old, about 6 months.


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OfflineCamtaro420
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Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 5 years, 5 months
Re: Bacteria on Rye Spawn? [Re: joegibbs]
    #19399054 - 01/10/14 01:18 PM (10 years, 20 days ago)

Quote:

joegibbs said:
Quote:

How did you prepare the rye though?

was it dry enough when placed inside the jar?

was it PC'd or steamed and how long either way?




Inoculated in sterile glove box, with needle going into alcohol between each jar. 

The spore syringe I used was a bit old, about 6 months.




Well the alcohol instead of flame between each jar could have fucked you over.

Or the MS syringe that was 6 months old.

Next time use flame till red hot between each jar, use alcohol on things you touch with your hands only, never the needle of the syringe nothing is better or safer then flame.

Also use isolated strains on agar for grains and leave the syringes for making cakes.


--------------------


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Offline2bittoker
Resident PMP Advocate


Registered: 03/09/13
Posts: 555
Last seen: 2 years, 3 months
Re: Bacteria on Rye Spawn? [Re: Camtaro420]
    #19399089 - 01/10/14 01:25 PM (10 years, 20 days ago)

Quote:

Camtaro420 said:
Quote:

joegibbs said:
Quote:

How did you prepare the rye though?

was it dry enough when placed inside the jar?

was it PC'd or steamed and how long either way?




Inoculated in sterile glove box, with needle going into alcohol between each jar. 

The spore syringe I used was a bit old, about 6 months.




Well the alcohol instead of flame between each jar could have fucked you over.

Or the MS syringe that was 6 months old.

Next time use flame till red hot between each jar, use alcohol on things you touch with your hands only, never the needle of the syringe nothing is better or safer then flame.

Also use isolated strains on agar for grains and leave the syringes for making cakes.



This.  Alcohol only Sanitizes, it does not Sterilize.  Its only effective against bacteria (and only in 70/30 mixture).  It does nothing to mold or mold spores.  I dont use alcohol on my needles at all any more, only flame.


--------------------
“I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.”
"Love does not claim possession, but gives freedom"
― Rabindranath Tagore

Stuff for New Growers
Where new growers should start: RogerRabbit's PF Tek video    How it Should and Shouldn't Look 
My Simplified Bulk Growing  My OJ Shroom Tek


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Bacteria on Rye Spawn? [Re: Camtaro420]
    #19399131 - 01/10/14 01:33 PM (10 years, 20 days ago)

Quote:

Camtaro420 said:
Well the alcohol instead of flame between each jar could have fucked you over.

Or the MS syringe that was 6 months old.

Next time use flame till red hot between each jar, use alcohol on things you touch with your hands only, never the needle of the syringe nothing is better or safer then flame.

Also use isolated strains on agar for grains and leave the syringes for making cakes.



This is great information!

As Camtaro stated, the only use of alcohol is for rubber gloves, and wiping down a scalpel prior to flaming the blade red hot.  If you are in a SAB, it certainly never hurts to wipe down the jars etc prior to placing them in the SAB....But ISO alcohol is not a "kill-all" like fire is.


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Offline2bittoker
Resident PMP Advocate


Registered: 03/09/13
Posts: 555
Last seen: 2 years, 3 months
Re: Bacteria on Rye Spawn? [Re: HypnotoadCroaked]
    #19399167 - 01/10/14 01:39 PM (10 years, 20 days ago)

Quote:

Whippy said:
Quote:

Camtaro420 said:
Well the alcohol instead of flame between each jar could have fucked you over.

Or the MS syringe that was 6 months old.

Next time use flame till red hot between each jar, use alcohol on things you touch with your hands only, never the needle of the syringe nothing is better or safer then flame.

Also use isolated strains on agar for grains and leave the syringes for making cakes.



This is great information!

As Camtaro stated, the only use of alcohol is for rubber gloves, and wiping down a scalpel prior to flaming the blade red hot.  If you are in a SAB, it certainly never hurts to wipe down the jars etc prior to placing them in the SAB....But ISO alcohol is not a "kill-all" like fire is.


Its not only not a kill all, it does nothing against your main enemy: Trich.  The solvent properties of Alcohol help clean gloves of mold spores, but it doesnt kill them.


--------------------
“I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.”
"Love does not claim possession, but gives freedom"
― Rabindranath Tagore

Stuff for New Growers
Where new growers should start: RogerRabbit's PF Tek video    How it Should and Shouldn't Look 
My Simplified Bulk Growing  My OJ Shroom Tek


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Offlinejoegibbs
Stranger
Registered: 12/30/12
Posts: 23
Last seen: 5 years, 5 months
Re: Bacteria on Rye Spawn? [Re: 2bittoker]
    #19399180 - 01/10/14 01:40 PM (10 years, 20 days ago)

I've fought trich several times in the past.  Good to know these things.  I've got the jars in the PC to kill it.  Won't try to salvage it.  I've got the Agar video, I'll give that a watch and see what I need to do that. 

Thanks guys.


Edited by joegibbs (01/10/14 01:42 PM)


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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: Bacteria on Rye Spawn? [Re: joegibbs]
    #19399258 - 01/10/14 01:50 PM (10 years, 20 days ago)

that doesn't even look like cube myc to me..
I'd use another inoculant/syringe next time.


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OfflineCamtaro420
Birdman
Male


Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 5 years, 5 months
Re: Bacteria on Rye Spawn? [Re: joegibbs]
    #19399345 - 01/10/14 02:07 PM (10 years, 20 days ago)

Quote:

joegibbs said:
I've fought trich several times in the past.  Good to know these things.  I've got the jars in the PC to kill it.  Won't try to salvage it.  I've got the Agar video, I'll give that a watch and see what I need to do that. 

Thanks guys.




You've got alot to learn. I suggest visiting these two threads

http://www.shroomery.org/forums/showflat.php/Number/18430998

http://www.shroomery.org/forums/showflat.php/Number/18219379#18219379

Try it in this order.

Syringe -> PF Tek/BRF Cakes -> SGFC Fruiting -> Fruit Harvesting for Cloning -> Agar Cloning and Isolation -> Isolate to Grain Colonization -> G2G for more Spawn ->

Then from there you can do whatever with all that spawn. Just make sure to proper sterilization techniques. Seems like you had it down well except for using the alcohol instead of flame.

Always flame the tools that can be!


--------------------


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