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robbinbanks
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Registered: 12/23/13
Posts: 64
Loc: Great Plains
Last seen: 9 years, 9 months
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only 1 cake to birth, best method?
#19398493 - 01/10/14 11:26 AM (10 years, 21 days ago) |
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well its looking like i will have 1 out of 12 brf jars fully colonize , so, what would be the best way to birth and fruit it? I have a sgfc but it seems like it would be hard to keep the humidity up. I want the most risk free way to birth one cake so i can atleast start my own prints and isolates. I also have a b+ syringe in the fridge from *****. Can i use a store bought syringe for agar, liquid culture, rye berrie jars? Or with the store bought, should i only stick to brf cakes. (i dont care for the brf cakes after the last attempt and wanna switch it up if possible)
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Edited by robbinbanks (01/10/14 03:48 PM)
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Camtaro420
Birdman



Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 5 years, 5 months
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Re: only 1 cake to birth, best method? [Re: robbinbanks]
#19398833 - 01/10/14 12:39 PM (10 years, 20 days ago) |
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First take the spore vendor's name out.
I can't say much for the birthing other then if it's been consolidated for a week and the others aren't close it's prolly good to go, just set him up in a FC and wait for the others, i'm sure you could prolly wait a lil' longer though. Wait to see what a Pro says.
As for the other other stuff
Don't go straight to agar with the syringe. Just make more cakes. Use your soon to be fruiting cakes to make clones/isolates with. Skip the LC all together, no reason for it. Once you have good isolates just take from that agar then add it to the rye or whatever grains, then take it from there.
Edit: although if I remember correctly, I told you this yesterday lol.
Edited by Camtaro420 (01/10/14 12:40 PM)
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tripmob
strang and unusual



Registered: 01/04/14
Posts: 176
Last seen: 7 years, 4 months
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Re: only 1 cake to birth, best method? [Re: Camtaro420]
#19398902 - 01/10/14 12:51 PM (10 years, 20 days ago) |
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Idk...the safest way?...if your just looking to get some shrooms for whatever reason, you can leave it in the jar and let the shrooms spawn "in vitro" check out zoomingtotheedge on youtube. I know its not the best way, but its deffinatly the easiest.
-------------------- Theres only 2 things I hate in life, Racism and Chinese People. 10, 000 rocks of crack cocaine is too much!
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robbinbanks
Not a Stranger


Registered: 12/23/13
Posts: 64
Loc: Great Plains
Last seen: 9 years, 9 months
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Re: only 1 cake to birth, best method? [Re: Camtaro420]
#19399686 - 01/10/14 03:29 PM (10 years, 20 days ago) |
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why do i have to take the spore vendors name out? and how do i? i really would like to skip the pf tek, but might do a couple pf jars, some rye, and agar. I mean, i just need one mushroom to start my isolates lol. I did the substitute for agar or whatever (brf and water) and i got some white myc starting up from some left over spores in an old syringe i kept in the freezer, so might be able to use that too. Why is it that you cant effectively use store bought multi spore for rye? I guess i dont see why it would be more effective in a pf jar? I have looked everywhere and cant find the info to back that theory. What would jesus do? lol
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Camtaro420
Birdman



Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 5 years, 5 months
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Re: only 1 cake to birth, best method? [Re: robbinbanks]
#19399752 - 01/10/14 03:42 PM (10 years, 20 days ago) |
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Quote:
robbinbanks said: why do i have to take the spore vendors name out? and how do i? i really would like to skip the pf tek, but might do a couple pf jars, some rye, and agar. I mean, i just need one mushroom to start my isolates lol. I did the substitute for agar or whatever (brf and water) and i got some white myc starting up from some left over spores in an old syringe i kept in the freezer, so might be able to use that too. Why is it that you cant effectively use store bought multi spore for rye? I guess i dont see why it would be more effective in a pf jar? I have looked everywhere and cant find the info to back that theory. What would jesus do? lol
The spore vendor sells those for scientific use not for growing and it could lead to them getting cited.
There's a few reasons why you don't want to use a MS syringe for grain innoc when you could use agar wedges instead.
Skipping to my personal main reasons for suggesting against it is two reasons.
one - contamination. Unless you clean the growth before adding it to grain, you're running a high risk of growing something from that syringe you don't want.
two - agar isolation is an easy way to perfecting your bulk grow. Not doing it is fine, but doing it yeilds far better results.
Going from Syringe -> Cakes -> Fruiting -> Agar Clone/Isolation -> Grains is the safest and most popular means to an end from scratch I have found on this forum.
Edited by Camtaro420 (01/10/14 03:43 PM)
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robbinbanks
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Registered: 12/23/13
Posts: 64
Loc: Great Plains
Last seen: 9 years, 9 months
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Re: only 1 cake to birth, best method? [Re: Camtaro420]
#19399809 - 01/10/14 03:52 PM (10 years, 20 days ago) |
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makes sense. What is "clean growth"? and i plan on working up to agar. Im just terrified im gonna loose my jars to trich, but then again i inoculated those in open air lol.I think ill give the pf jars another chance and do it right this time. If that fails im coming to your house and stealing your albinos, ha jk
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Camtaro420
Birdman



Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 5 years, 5 months
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Re: only 1 cake to birth, best method? [Re: robbinbanks]
#19399930 - 01/10/14 04:09 PM (10 years, 20 days ago) |
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Quote:
robbinbanks said: makes sense. What is "clean growth"? and i plan on working up to agar. Im just terrified im gonna loose my jars to trich, but then again i inoculated those in open air lol.I think ill give the pf jars another chance and do it right this time. If that fails im coming to your house and stealing your albinos, ha jk
Lol, I'm still in the same boat as you. Once I have fruits growing I will transfer them to agar so I can isolate to ensure nothing else (like trich) is growing on the dishes with the myc.
Once the myc has fully colonized an agar dish and it looks clean strong and healthy that is considered "clean growth" (this can take a few transfers). Then you can cut it up with a scalpel that has been flame sterilized and transfer it to a grain jar.
provided this is all in a SAB and other sterile procedures were taken as well.
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robbinbanks
Not a Stranger


Registered: 12/23/13
Posts: 64
Loc: Great Plains
Last seen: 9 years, 9 months
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Re: only 1 cake to birth, best method? [Re: Camtaro420]
#19400230 - 01/10/14 05:04 PM (10 years, 20 days ago) |
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i cant wait, ill be sure to share pics with everyone!
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