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Just for Fun The In-vitro grain jar
    #19398085 - 01/10/14 09:43 AM (10 years, 21 days ago)

Well I left one grain jar with the lid slightly loosened after full colonization just to see how long it would take to pin in-vitro. This one took two weeks. I'm thinking of putting it in the SGFC but right now I have the lid-ring holding on a single layer of paper towel and I give it a little water every day.



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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19398172 - 01/10/14 10:03 AM (10 years, 21 days ago)

He looks like he has been a very naughty mushroom somehow :awesome:


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19398184 - 01/10/14 10:04 AM (10 years, 21 days ago)

It would likely pin faster and more with a little casing layer inside.  It also can be watered after it shrinks some.  Though it would be better if the grain were about half as high loaded.


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Re: Just for Fun The In-vitro grain jar [Re: Violet]
    #19398325 - 01/10/14 10:38 AM (10 years, 20 days ago)

Quote:

Violet said:
It would likely pin faster and more with a little casing layer inside.  It also can be watered after it shrinks some.  Though it would be better if the grain were about half as high loaded.



I did it for the glam not the yield. But I'm sure you're right. I'm not too interested in doing this ever again, I really just wanted to see how long it would take for a regular grain jar to say fuck this I'm doing my thing even if you don't want to pay attention to me,


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19398366 - 01/10/14 10:49 AM (10 years, 20 days ago)

Gotcha.  But it actually works very well as a singular grow method.  I did it with glass lots before discovering how much better and easier it was with plastic and their screw-on lids.

Surprisingly I find that casing layers can decrease the time until pinning.  Sortof as if the fruits delay because they want a low-nutrition pinning site, as they already have saturation humidity throughout.
Yep, I'm referring to dropping in a casing layer into a light-loaded container.  I'll also be doing a larger experiment/demo with it.

Try it sometime! Super easy


--------------------
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Re: Just for Fun The In-vitro grain jar [Re: Violet]
    #19398376 - 01/10/14 10:53 AM (10 years, 20 days ago)

Quote:

Violet said:
Gotcha.  But it actually works very well as a singular grow method.  I did it with glass lots before discovering how much better and easier it was with plastic and their screw-on lids.

Surprisingly I find that casing layers can decrease the time until pinning.  Sortof as if the fruits delay because they want a low-nutrition pinning site, as they already have saturation humidity throughout.
Yep, I'm referring to dropping in a casing layer into a light-loaded container.  I'll also be doing a larger experiment/demo with it.

Try it sometime! Super easy




Wouldn't it be easier just pour the grains very shallowly into a tub and then put a small casing layer on it then put the lid back on and fruit it like it's in colonization then? It's like having a bunch of your in-vitro plastics in a big in-vitro plastic.


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19398383 - 01/10/14 10:56 AM (10 years, 20 days ago)

:thumbup:


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19398552 - 01/10/14 11:39 AM (10 years, 20 days ago)

Quote:

bodhisatta said:
Wouldn't it be easier just pour the grains very shallowly into a tub and then put a small casing layer on it then put the lid back on and fruit it like it's in colonization then? It's like having a bunch of your in-vitro plastics in a big in-vitro plastic.



No, it would Not be easier.
One would already be preparing and laying casing layer; your idea involves a cleaned tub, breaking up and laying out grains, and a recovery period before the casing layer is colonized, by a culture which is sortof brand-new, the mycelium that was embedded in the surface of the grains having to re-start the life cycle from scratch.  The straight-grains just simply don't knit back together the same either.
And it's simply unnecessary to destroy the original ideal growth on grains, particularly just to put one's eggs all in one basket that all must be tossed at once when contams occur, which becomes more likely or sooner due to the less healthy mycelium.
Additionally, such a giant thing would have watering problems.  The edges near the water would be too far from the majority of the substrate in the middle.  Especially considering that conditions may be more difficult to maintain as well, particularly seeing as I NEVER have to mist my invitros.  It's way easy and very assured success.


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Re: Just for Fun The In-vitro grain jar [Re: Violet]
    #19398625 - 01/10/14 11:55 AM (10 years, 20 days ago)

So, I'm posting a topic tonight with pics of my crazy invitro experiment. Got pins and shrooms all over it.
I had some grains left over after g2g, threw some dry verm, and some water, shook and let sir around with a lid and sfd on. Looked at it couple days ago and bam! Fruits.


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Re: Just for Fun The In-vitro grain jar [Re: blojo02184]
    #19398634 - 01/10/14 11:56 AM (10 years, 20 days ago)

Quote:

blojo02184 said:
So, I'm posting a topic tonight with pics of my crazy invitro experiment. Got pins and shrooms all over it.
I had some grains left over after g2g, threw some dry verm, and some water, shook and let sir around with a lid and sfd on. Looked at it couple days ago and bam! Fruits.



Cubes really like to grow no matter what you do to them :thumbup:


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19398899 - 01/10/14 12:50 PM (10 years, 20 days ago)

fuck i left my ksss jar for four months under 12/12 and couldn't get it to pin:mad2:
mind you i wasn't around to give it fresh air as much(maybe once a week)


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Re: Just for Fun The In-vitro grain jar [Re: cronicr]
    #19399203 - 01/10/14 01:43 PM (10 years, 20 days ago)

Casing layer! It's like magic for that stuff.  I think they don't like pinning on straight grains as much as with a low-nutrition site.  I wouldn't be surprised if that jar would have fruited in the same setup except with a casing.  Maybe it would need more air though.


--------------------
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Re: Just for Fun The In-vitro grain jar [Re: Violet]
    #19399226 - 01/10/14 01:46 PM (10 years, 20 days ago)

You guys got me motivated to put it together just now.

Enjoy Bod,

Violet, im sure you will tell me plastics...

Voila


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Re: Just for Fun The In-vitro grain jar [Re: Violet]
    #19399240 - 01/10/14 01:48 PM (10 years, 20 days ago)

lol yeah i was inbetween fishing trips, had i known i could just nuke some potting soil i may have tried lol.
but i mainly wanted to see y ambient lighting is always recommended and not direct and intense and the ambient lighting for sure gave a different growth then the direct, i have pics somewhere of it i had the first week in total darkness the second week 12/12 with ambient lighting and week 3 and right to 4 months on direct intense, was pretty neat to see the different effects of lighting on colonizing grain jars(i never shook the jar once) and had i not fucked the first flush up it would have gotten me well over a couple z's still :crazy:


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Re: Just for Fun The In-vitro grain jar [Re: cronicr]
    #19399465 - 01/10/14 02:38 PM (10 years, 20 days ago)

I wonder how big that fruit will get.


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19400285 - 01/10/14 05:17 PM (10 years, 20 days ago)

Have you soaked the grains at all yet?


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Re: Just for Fun The In-vitro grain jar [Re: Kizzle]
    #19400291 - 01/10/14 05:19 PM (10 years, 20 days ago)

Quote:

Kizzle said:
Have you soaked the grains at all yet?




I have not, I watered them until I got slight pooling at the bottom, and then I tried to roll it around gently to distribute it evenly, I gave it a little bit of a misting today.


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19408014 - 01/12/14 11:53 AM (10 years, 18 days ago)





Sorry it's rotated all dumb but I'm to lazy to fix it.


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19408054 - 01/12/14 12:02 PM (10 years, 18 days ago)

i don't know y i always feel obligated to turn my head sideways lol


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Re: Just for Fun The In-vitro grain jar [Re: cronicr]
    #19408110 - 01/12/14 12:16 PM (10 years, 18 days ago)

Quote:

cronicr said:
i don't know y i always feel obligated to turn my head sideways lol



Fucking brains, how do they work :shrug:


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19408476 - 01/12/14 01:56 PM (10 years, 18 days ago)

Looks like you got a couple tiny ones popping up too

I wonder if it will be mostly hollow or mostly full?


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Re: Just for Fun The In-vitro grain jar [Re: blojo02184]
    #19408534 - 01/12/14 02:10 PM (10 years, 18 days ago)

Quote:

blojo02184 said:
Looks like you got a couple tiny ones popping up too

I wonder if it will be mostly hollow or mostly full?




yea there's about 10-20 starting all around the jar now. I would imagine it's going to be quite hollow given the girth of the stipe.


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19408923 - 01/12/14 03:52 PM (10 years, 18 days ago)

True, but it looks like its jump off point is larger than normal.

Don't know the term for base of stem...?


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Re: Just for Fun The In-vitro grain jar [Re: blojo02184]
    #19409613 - 01/12/14 06:40 PM (10 years, 18 days ago)

Quote:

blojo02184 said:
True, but it looks like its jump off point is larger than normal.

Don't know the term for base of stem...?




We'll just call it where the cock meets the balls, or the base of the stem. I'll dissect it when it's ready for harvest probably with my teeth


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19409665 - 01/12/14 06:57 PM (10 years, 18 days ago)

he'll dissect with his teeth...
:punkband: 
where the cock meets the balls...


a line from an 80's power ballad?
:rockman:


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Re: Just for Fun The In-vitro grain jar [Re: cronicr]
    #19409684 - 01/12/14 07:01 PM (10 years, 18 days ago)

Quote:

cronicr said:
i don't know y i always feel obligated to turn my head sideways lol




fucken taco neck


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Re: Just for Fun The In-vitro grain jar [Re: CMOS]
    #19417073 - 01/14/14 12:06 PM (10 years, 16 days ago)

update pics





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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19417687 - 01/14/14 02:49 PM (10 years, 16 days ago)

That is one slow growing fruit it seems.
Its been what, a week about?, my invitro experiment popped out mature fruits in like 5 or 6 days.


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Re: Just for Fun The In-vitro grain jar [Re: blojo02184]
    #19417699 - 01/14/14 02:52 PM (10 years, 16 days ago)

:whistling:


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Re: Just for Fun The In-vitro grain jar [Re: blojo02184]
    #19417701 - 01/14/14 02:52 PM (10 years, 16 days ago)

Its cold still like 62-65 in my house lately


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19419418 - 01/14/14 08:10 PM (10 years, 16 days ago)

After massacring my experiment, I dumped some water in and let it sit back down.

You going to try for a second flush?


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Re: Just for Fun The In-vitro grain jar [Re: blojo02184]
    #19419608 - 01/14/14 08:52 PM (10 years, 16 days ago)

Lets get a second flush going!


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Re: Just for Fun The In-vitro grain jar [Re: toawbe]
    #19419621 - 01/14/14 08:53 PM (10 years, 16 days ago)

I'll keep watering it as needed and let it go until it contams or rots or whatever else.


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19432806 - 01/17/14 11:29 AM (10 years, 13 days ago)






harvested the first guy now there's plenty more popping up, sort of ugly but W/e


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19433013 - 01/17/14 12:14 PM (10 years, 13 days ago)

I've done this before many times with varying success and I always got at least 4 flushes out of each jar even 6 flushes in 2 different jars with less fruits in each flush after the 2nd. when you water make sure not to let the water puddle and sit for long periods or it will leave a brown slimy spot around the bottom of the grain IME. I also noticed if you let the jar consolidate for around a month and a half or even longer the mushrooms will be extremely potent in tiny doses, I tripped Balls on just 2 small to medium sized mushrooms grown this way.


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Re: Just for Fun The In-vitro grain jar [Re: LordChaos]
    #19433029 - 01/17/14 12:18 PM (10 years, 13 days ago)

Quote:

LordChaos said:
I also noticed if you let the jar consolidate for around a month and a half or even longer the mushrooms will be extremely potent



;]


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

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Re: Just for Fun The In-vitro grain jar [Re: LordChaos]
    #19433147 - 01/17/14 12:45 PM (10 years, 13 days ago)

I meant to add that the fruits seem to grow fairly large and long, the biggest fruits I've ever had have come from this type of in-vitro jar often growing an inch or more out the top of the jar but no matter how much room the caps had to open up none of my caps fully matured or opened fully and produced spores. I'd like to add that the mushrooms will eventually grow lower and lower down the sides of the grain making them harder to pick without significant damage to the substrate.


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19658247 - 03/06/14 06:03 AM (9 years, 10 months ago)

Hey! How it's going the experiment? Still flushing?
I'm amazed I just have a grain jar that started to pin invitro and entered the site to get some advice... and found this thread! YEAH! That's GREAT to have sites like this...

I'm not sure if I'll add a casing layer, but provided your published results it's worth trying to reproduce your experiment... Anyway, any advice about casing? I would prefer something simple, perhaps 50/50 coco/verm? I know (from theory) about more elaborate casings, but I would like to keep as simple as possible... so most probably I will go with any... but just for advice...
thanks everybody!
PS: I'll try to add a picture if you allow me to do it


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Re: Just for Fun The In-vitro grain jar [Re: MelMush]
    #19658519 - 03/06/14 08:44 AM (9 years, 10 months ago)

it's much better to just spawn the grain to a shallow tray and put a casing on it you'll greatly improve your yields. This was just done for the hell of it


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19658541 - 03/06/14 08:53 AM (9 years, 10 months ago)

It's been my experience that 750mL volume of prepared grain yields much better divided into 3 quart containers, cased and watered inside them, than in one quart container then broken-up and lain out cased in a tray with no good watering method


--------------------
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Re: Just for Fun The In-vitro grain jar [Re: Violet]
    #19658565 - 03/06/14 09:05 AM (9 years, 10 months ago)

since when is a spray bottle not a good watering method.


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19658620 - 03/06/14 09:24 AM (9 years, 10 months ago)

Since Violet said so...... duh!


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Re: Just for Fun The In-vitro grain jar [Re: PussyFart]
    #19658625 - 03/06/14 09:26 AM (9 years, 10 months ago)

Hey v,
Was that higher BE test done with cubes only? Or did you happen to try exotics that way... Galindoi?


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Re: Just for Fun The In-vitro grain jar [Re: blojo02184]
    #19658663 - 03/06/14 09:35 AM (9 years, 10 months ago)

Quote:

blojo02184 said:
Hey v,
Was that higher BE test done with cubes only? Or did you happen to try exotics that way... Galindoi?



Here's another question, How come farms(including cube farms when cubes were legal in Amsterdam/now they make truffles since those are legal still)  that have to worry about profit margins, supply expenses, time management, and space management all find themselves using bulk methods :shrug:


Edited by Trusted cuItivator (03/06/14 09:37 AM)


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Re: Just for Fun The In-vitro grain jar [Re: bodhisatta]
    #19659262 - 03/06/14 12:44 PM (9 years, 10 months ago)

Quote:

bodhisatta said:
since when is a spray bottle not a good watering method.



Since droplets On the casing are not absorbed and used by the mycelium for mushrooms.

I don't expect you to know that yet, though, since you haven't yet done it and seen that spraying the casing is not a working alternative to bottom-watering.


Quote:

PussyFart said:
Since Violet said so...... duh!



Ass.

Quote:

bodhisatta said:
Quote:

blojo02184 said:
Hey v,
Was that higher BE test done with cubes only? Or did you happen to try exotics that way... Galindoi?



Here's another question, How come farms(including cube farms when cubes were legal in Amsterdam/now they make truffles since those are legal still)  that have to worry about profit margins, supply expenses, time management, and space management all find themselves using bulk methods :shrug:



Because it's what they know.
What alternative has there been?
"Cased grains" of broken-up and lain-out "spawn" with no proper watering?
Poor results. Bulk obviously beats that, despite the many extra steps and expenditures.

No blojo, tho the tek is meant primarily for Cubensis it does work well for other grain-fruiting species like Galindoi but I don't keep track of numbers. I did get nice strong consecutive flushing with Galindoi though


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