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myco_man
ametur

Registered: 12/14/13
Posts: 4
Last seen: 9 years, 11 months
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Hypothetical BRF&Verm. Tek question
#19397712 - 01/10/14 07:46 AM (10 years, 21 days ago) |
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Hello fellow mycologists. This is my first post and to start I'm am very excited to speak with people that share a love and similar interest as I do, and would like to pre-thank all of those that respond to my post. Your help and knowledge is greatly appreciated. My question is as follows; hypothetically can one using the brown rice flower and Verm. TEK safely and successfully use a small amount of healthy rizomorphic mycelium from one jar to inoculate a jar that isn't showing signs of any growth after a 20 day period without destroying the contents of the parent jar so to speak?
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Hypothetical BRF&Verm. Tek question [Re: myco_man]
#19397756 - 01/10/14 08:01 AM (10 years, 21 days ago) |
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I'm sure it can be done. the parent jar would need to be at 100% colonization tho for sure. do it in an sab, with the other, sterilized brf jars there too, perform a g2g thing. the dry verm layer would be a problem though...do u pc or steam?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Hypothetical BRF&Verm. Tek question [Re: myco_man]
#19397779 - 01/10/14 08:08 AM (10 years, 21 days ago) |
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Quote:
myco_man said: Hello fellow mycologists. This is my first post and to start I'm am very excited to speak with people that share a love and similar interest as I do, and would like to pre-thank all of those that respond to my post. Your help and knowledge is greatly appreciated. My question is as follows; hypothetically can one using the brown rice flower and Verm. TEK safely and successfully use a small amount of healthy rizomorphic mycelium from one jar to inoculate a jar that isn't showing signs of any growth after a 20 day period without destroying the contents of the parent jar so to speak?
In short no, 1. The dry verm barrier on the top is full of contaminants, When you move the jar around too much you can jostle the rogue spores down into the BRF mix part of your jars, The parent jar likely wouldn't contaminate since colonized substrates are resistant to contamination. The thing is when that mycelium goes into the other jar it's going to have contaminant spores on it and destroy the new jar. 2. Even if you tried to do what was in #1 you would have to do all of it in a Still Air Box(SAB) this is the next best tool to a Laminar Flow Hood(LFH) which is really the only two good ways to keep your work sterile enough to avoid failure. 3. Both jar have a dry verm barrier so your chances of failure are doubled and the chance was already high to begin with. You'll have to remove the barrier from both jars and that's a lot of un-sterile movements and bad technique etc.. bad news bears basically.
Edited by Trusted cuItivator (01/10/14 08:09 AM)
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myco_man
ametur

Registered: 12/14/13
Posts: 4
Last seen: 9 years, 11 months
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Re: Hypothetical BRF&Verm. Tek question [Re: bodhisatta]
#19423959 - 01/15/14 05:31 PM (10 years, 15 days ago) |
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What is the best method to maintain the correct temperature for cakes in a fruiting chamber?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Hypothetical BRF&Verm. Tek question [Re: myco_man]
#19423966 - 01/15/14 05:32 PM (10 years, 15 days ago) |
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heat the room up
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Happy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 1 year, 2 months
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Re: Hypothetical BRF&Verm. Tek question [Re: myco_man]
#19424021 - 01/15/14 05:43 PM (10 years, 15 days ago) |
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Maybe take the stuff that didn't grow and start on an outside patch that you can colonize later with the addition of spent cakes that may be still active?
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myco_man
ametur

Registered: 12/14/13
Posts: 4
Last seen: 9 years, 11 months
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Thanks for all the good answers guys. I have another question. Let's say some jars colonize faster than others can one wait for all jars to colonize 100% before fruiting, and if not then why.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Hypothetical BRF&Verm. Tek question [Re: myco_man]
#19434559 - 01/17/14 06:47 PM (10 years, 13 days ago) |
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of course you can wait
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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myco_man
ametur

Registered: 12/14/13
Posts: 4
Last seen: 9 years, 11 months
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Re: Hypothetical BRF&Verm. Tek question [Re: cronicr]
#19436802 - 01/18/14 08:37 AM (10 years, 13 days ago) |
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Thanks a lot chronicr. I hypothesized that the more advanced jars may have degenerated in some way while waiting for other jars to mature.
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