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Invisiblemushmagic
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Re: My first cased pins :) [Re: Cogsy]
    #19392062 - 01/09/14 04:10 AM (10 years, 1 month ago)

I'd personally skip the LC and go with agar. LCs are more trouble than they're worth most of the time. You'll never be able to tell if an LC is clean just by looking at it, you'll need to shoot up a couple test jars to find out and if it's not clean then you wasted all that time and you're back to square one.


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OfflineCogsy
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Re: My first cased pins :) [Re: mushmagic]
    #19392087 - 01/09/14 04:18 AM (10 years, 1 month ago)

I know man :frown: but I'm just starting this mycological expedition, I really want to work with agar and feel like a proper scientist :wink: but I don't have the resources yet :/ I'm debating wether to get a decent PC on payday or get a mini fridge and start using agar (my parents won't let me keep loads of live petri's in my fridge :P)
But for now I have honey water and mycelium :smile: and I'm 4/4 on my LC's so far.. I'll make a couple to insure I get atleast one :smile:


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great write-up for new-guys :smile:
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Invisiblemushmagic
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Re: My first cased pins :) [Re: Cogsy]
    #19392113 - 01/09/14 04:29 AM (10 years, 1 month ago)

Well best of luck to you in your endeavors and glad to hear you're 4 for 4 on your LCs:thumbup: And yes I'd definitely pick up a PC! An All American wont let you down if your funds allow for it.


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Trade list in journal (partially under construction; more to be added)


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OfflineCogsy
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Re: My first cased pins :) [Re: mushmagic]
    #19392163 - 01/09/14 04:52 AM (10 years, 1 month ago)

was looking at an AA 25qt, but that's a fair bit in the UK, 200-250 I think depending on shipping and imort fees D: probably worth it though ;D

that's pounds as well, not dollars D:


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great write-up for new-guys :smile:
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Cogsy


Edited by Cogsy (01/09/14 04:53 AM)


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OfflineCogsy
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Re: My first cased pins :) [Re: Cogsy]
    #19392191 - 01/09/14 05:03 AM (10 years, 1 month ago)

just a quick question too :P
how viable is agar work in a glovebox? will the still air provide enough protection? or will you end up having to do more petri's before you can get a clean rhizo mycelium?


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great write-up for new-guys :smile:
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Cogsy


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Invisibleblindingleaf
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Re: My first cased pins :) [Re: Cogsy]
    #19392242 - 01/09/14 05:19 AM (10 years, 1 month ago)

its easy in an SAB.  u will have some transfers to do at times to clean up a culture, and then more transfers to get an isolate.  in ur case, putting clone tissue from the inner stem onto agar , letting it grow to a dime sized piece then making one more transfer to ensure its clean, will be a lot like u making an lc with clone tissue, its not an isolate (though u may get lucky) but its a clean set of genetics that has fruited for u before, and would be glad to fruit for you again :smile:

ps spring for the PC instead of mini fridge!  u need a pc anyway to start agar work.


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MICROBIAL HUSBANDRY!!!!

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OfflineCogsy
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Re: My first cased pins :) [Re: blindingleaf]
    #19392363 - 01/09/14 06:04 AM (10 years, 1 month ago)

I'll probably buy an agar kit the same time I get a PC then :') theyre not that expensive.... I'll just hide my petri dishes in my fridge :wink:

just a question about this tissue LC im about to do, taking a very small sample of mycelium should mean I'll be Isolating to minimal different strains yeah? and taking the mycelium from inside the best fruit body should mean that the strains I'll be developing are favourable... theoretically?
or have I missed something..?


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great write-up for new-guys :smile:
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Cogsy


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Invisibleblindingleaf
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Re: My first cased pins :) [Re: Cogsy]
    #19392436 - 01/09/14 06:35 AM (10 years, 1 month ago)

I'm no expert, but i will try my best to help
yes, by taking a piece of tissue, u r limiting the genetics more than an MS LC.  i would take a piece from a smaller fruit body in a cluster myself, but only because it is set to expand and grow, rather than to produce spores, like with a mature fruit, though i have done both (never to an LC though, be careful) and not noticed a difference on agar anyway (btw smaller fruits and mature fruits) in the rate of growth, just  theoretically makes sense to me.
im not sure what the fact/opinion/experience is on this but i'd say large fruit bodies r equally environmental as much as genetics.  a bulk sub will produce larger fruits because of the water within it, so if u clone  a large fruit from that, and put it in a drier substrate, it will not grow as big, esp when considering it is not a true isolate, just a clone
sry is that last part made no sense, I'm just waking up here and think i have a cold


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineCogsy
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Re: My first cased pins :) [Re: blindingleaf]
    #19392471 - 01/09/14 07:00 AM (10 years, 1 month ago)

I get what you're saying :laugh: makes sense, as the pins grow quicker than the bigger ones their mycelium might make a better LC, when it comes down to genetics though, wouldn't you be better off knowing how the mushroom you re 'cloning' act's during it whole life cycle?
and don't smaller mushrooms expand and grow quickly because they are small mushrooms and need to grow? this is just my thoughts but wouldn't the mycelium inside the mushroom be the same mycelium throughout it's life cycle, with the same genetics, and once introduced to agar or LC it will start growing rapidly again? maybe there's a small wait where the mycelium from a larger mushroom has to change what it's doing (from spore creation to colonization)and that would account for the visible increase in growth between the immature and the mature samples? as the immature mycelium is still in its growing period when introduced to LC or agar? I'm new to mycology so I'm probably completely wrong... but they're my attempts scientific thoughts :')


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great write-up for new-guys :smile:
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____________________________________________________________

Cogsy


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Invisibleblindingleaf
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Re: My first cased pins :) [Re: Cogsy]
    #19392501 - 01/09/14 07:16 AM (10 years, 1 month ago)

yes its nice to know how it will act, but since it is a clone, and more than likely multiple sets of genetics at play, its tough to say from one grow to the next if it will perform similarly, whereas with an isolate, u know it is one set of genetics that will be more predictable. so taking from a mature fruit will let u know how it MIGHT look, but taking from a smaller fruit will (i think) give u theoretically younger, more vigorous mycelium.
as far as the question "is a younger mushroom genetically equivalent to its more mature state?"  I'm not sure, maybe someone can chime in here.  i don't know if, as the fruit matures, it continuously gathers up more genetic material or not, or if it starts to pin, those specific genetics "lock in" so to speak and a pin and its mature future self are identical genetically.


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

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OfflinecronicrFacebook
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Re: My first cased pins :) [Re: blindingleaf]
    #19392990 - 01/09/14 10:19 AM (10 years, 1 month ago)

lc cloning is a shitty way to go about it, use agar


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OfflineNakor420
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Re: My first cased pins :) [Re: cronicr]
    #19395564 - 01/09/14 07:39 PM (10 years, 1 month ago)

Quote:

cronicr said:
lc cloning is a shitty way to go about it, use agar



:whathesaid:


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Offlinetwistedty
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Re: My first cased pins :) [Re: cronicr]
    #19395621 - 01/09/14 07:49 PM (10 years, 1 month ago)

Quote:

cronicr said:
lc cloning is a shitty way to go about it, use agar




agar is easier to use than LC and you can clone and isolate :shrug:


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OfflineCogsy
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Re: My first cased pins :) [Re: twistedty]
    #19395891 - 01/09/14 08:45 PM (10 years, 1 month ago)

Would if I could guys :') already said that I can't.. In my opinion beats making another MS syringe


--------------------
great write-up for new-guys :smile:
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Cogsy


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OfflinePussyFart
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Re: My first cased pins :) [Re: Cogsy]
    #19396208 - 01/09/14 10:12 PM (10 years, 1 month ago)

Making an LC beats making a spore syringe in your book?

lol


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OfflineNakor420
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Re: My first cased pins :) [Re: PussyFart]
    #19397294 - 01/10/14 04:39 AM (10 years, 1 month ago)

Quote:

Notahacker420 said:
Making an LC beats making a spore syringe in your book?

lol



:hehehe: ...glad I never read that book..


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OfflineCogsy
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Re: My first cased pins :) [Re: Nakor420]
    #19397478 - 01/10/14 06:21 AM (10 years, 1 month ago)

I already have 2 spore syringes for this strain, should've probably mentioned that at some point... Why all the hating? :') I don't have agar(getting it ASAP) it's not like LC doesn't work and isn't effective for inoculation.. I made 3 separate LC's with three different myc samples from the same mushroom.. If they all go to shit and harbour nothing but contaminants then yeah I'll just play it safer in future... I'm just doing what I can with the stuff I have..


--------------------
great write-up for new-guys :smile:
___________________________________________________________


____________________________________________________________

Cogsy


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OfflineNakor420
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Re: My first cased pins :) [Re: Cogsy]
    #19397635 - 01/10/14 07:19 AM (10 years, 1 month ago)

You should hold of on cloning and culture isolations until you get agar...lc is just a waste of time and material for most home cultivators...


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Offlinespacechildo
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Re: My first cased pins :) [Re: Cogsy]
    #19398075 - 01/10/14 09:41 AM (10 years, 1 month ago)

Quote:

Cogsy said:
I already have 2 spore syringes for this strain, should've probably mentioned that at some point... Why all the hating? :') I don't have agar(getting it ASAP) it's not like LC doesn't work and isn't effective for inoculation.. I made 3 separate LC's with three different myc samples from the same mushroom.. If they all go to shit and harbour nothing but contaminants then yeah I'll just play it safer in future... I'm just doing what I can with the stuff I have..




you took a tissue sample straight into lc? chancy...


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OfflineNakor420
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Re: My first cased pins :) [Re: spacechildo]
    #19398229 - 01/10/14 10:16 AM (10 years, 1 month ago)

If you are super sterile and careful and only take tissue from the core of the stem without infecting it then it's feasable...but not nearly optimal in terms of risk vs reward..


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