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Offlinerobbinbanks
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flipped stalled brf jars, birthing
    #19394362 - 01/09/14 03:57 PM (10 years, 21 days ago)

If you flip stalled brf jars, do you birth them bottom up? I hope i asked this right. I just read somewhere when your jars fully colonize you want to birth them right side up how they were when they colonized.


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OfflineKtulu369
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Re: flipped stalled brf jars, birthing [Re: robbinbanks]
    #19394416 - 01/09/14 04:13 PM (10 years, 21 days ago)

Hm, I've never heard/read that. Do you happen to remember where it was?

Also, I'm not sure what you mean by stalled; in this case, anyway. Usually when you say stalled, I'm pretty sure it means that the myc has stopped growing altogether, but that wouldn't be the case if the cakes are colonized.

I can't say I've ever heard of doing it differently based on how the cakes were oriented when colonizing. I only know of birthing them "lid side" down.


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OfflineCamtaro420
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Re: flipped stalled brf jars, birthing [Re: Ktulu369] * 1
    #19394452 - 01/09/14 04:22 PM (10 years, 21 days ago)

If it's stalled it's contaminated. Pics please. Don't flip it, that's stupid in any case. You shouldn't mess with it until it's 100% colonized, consolidated for a week, and hopefully invetro pinning.


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Offlinerobbinbanks
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Re: flipped stalled brf jars, birthing [Re: Ktulu369]
    #19394461 - 01/09/14 04:24 PM (10 years, 21 days ago)

well, they are not fully colonized yet, thats why i flipped them. I couldn't find where i read that but looked through RRs notes and apparently it doesn't matter, and he prefers lid down. I guess i thought the mycelium would prefer to be right side up considering it colonized that way during the 3 weeks or however long it took. Maybe i am over thinking again lol


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Offlinerobbinbanks
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Re: flipped stalled brf jars, birthing [Re: Camtaro420]
    #19394473 - 01/09/14 04:27 PM (10 years, 21 days ago)

It didnt stop completely, just colonizing really slow. I read rr notes

Non-contaminated cakes really only stall out if you leave the inoculation/gas exchange holes taped up with non-breathing tape, or make the jars too wet.  However, for the latter, it's OK to flip them, but you MUST leave them flipped until they finish.  If you disturb the verm filter, leave it that way.  If you flip them back to right-side-up, you put contaminated parts of the verm against the substrate.
RR

so i figured this was a reliable source


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OfflineCamtaro420
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Re: flipped stalled brf jars, birthing [Re: robbinbanks]
    #19394481 - 01/09/14 04:28 PM (10 years, 21 days ago)

Quote:

robbinbanks said:
well, they are not fully colonized yet, thats why i flipped them. I couldn't find where i read that but looked through RRs notes and apparently it doesn't matter, and he prefers lid down. I guess i thought the mycelium would prefer to be right side up considering it colonized that way during the 3 weeks or however long it took. Maybe i am over thinking again lol





:facepalm:
When RR says lid flipped down he is talking about when you are closing the lid. Not flipping the jar around so the cake is sitting on the lid... And like I said if the jar has stalled, flipping it isn't going to do shit to help, it's just going to hurt you more.


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Offlinerobbinbanks
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Re: flipped stalled brf jars, birthing [Re: Camtaro420]
    #19394513 - 01/09/14 04:34 PM (10 years, 21 days ago)

damn ha...well i lost 20 out of 24 jars because i didn't use a glove box, ordered a B+ syringe and im going to rye berries. There is so much info on here that it tends to get confusing... but thanks to everyones help im getting a clearer picture and i feel confident this next go around. Def no more flipping. thank you


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Offlinerobbinbanks
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Re: flipped stalled brf jars, birthing [Re: robbinbanks]
    #19394537 - 01/09/14 04:41 PM (10 years, 21 days ago)

RIP lol


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OfflineCamtaro420
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Re: flipped stalled brf jars, birthing [Re: robbinbanks]
    #19394578 - 01/09/14 04:49 PM (10 years, 21 days ago)

It's not the end of the world, honestly it might come out fine. It doesn't look like it's stalled just going slow.


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Edited by Camtaro420 (01/09/14 04:50 PM)


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Offlinerobbinbanks
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Re: flipped stalled brf jars, birthing [Re: Camtaro420]
    #19394590 - 01/09/14 04:52 PM (10 years, 21 days ago)

ya, i guess you gotta look at the glass half full...instead of half colonized lol


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OfflineCamtaro420
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Re: flipped stalled brf jars, birthing [Re: robbinbanks]
    #19394598 - 01/09/14 04:54 PM (10 years, 21 days ago)

Quote:

robbinbanks said:
damn ha...well i lost 20 out of 24 jars because i didn't use a glove box, ordered a B+ syringe and im going to rye berries. There is so much info on here that it tends to get confusing... but thanks to everyones help im getting a clearer picture and i feel confident this next go around. Def no more flipping. thank you




Also I wouldn't go straight to rye with a syringe.

Grow some cakes.

Get some fruits.

Transfer some fruits to clone on agar.

Isolate strong strains.

Use those isolates for master grain jars.

Then use those master grain jars to make tons more.

Then do whatever you want with spawn.

Back to original subject though, glove boxes are meh, use a still air box instead.

Now that those jars are flipped leave em be and let em grow see what happens.

There's nothing else you see growing in there right? I can't see anything other then myc, it's just slow bro.


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Offlinespacechildo
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Re: flipped stalled brf jars, birthing [Re: robbinbanks]
    #19394616 - 01/09/14 04:58 PM (10 years, 21 days ago)

what's the timeframe on these?
You need patience dude.
you've got the wrong kind of jars, that might be a reason they stalled.


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Offlinerobbinbanks
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Re: flipped stalled brf jars, birthing [Re: Camtaro420]
    #19394618 - 01/09/14 04:59 PM (10 years, 21 days ago)

i guess i do have a still air, it doesnt have gloves attached, and i dont see anything..lost 4 jars to trich before, and the others didnt do anything. What do you think about using a syringe for agar? Im getting agar and y plate petri dishes when i get my rye and gypsum...


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Offlinerobbinbanks
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Re: flipped stalled brf jars, birthing [Re: spacechildo]
    #19394628 - 01/09/14 05:00 PM (10 years, 21 days ago)

How are you gonna tell me to be patient when i didnt tell you the time frame yet? but, 3 and a half weeks, and you are probly right ha


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OfflineCamtaro420
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Re: flipped stalled brf jars, birthing [Re: robbinbanks]
    #19394640 - 01/09/14 05:03 PM (10 years, 21 days ago)

The jars have nothing to do with stalling, that's just silly. Unless they were like ridiculously huge or something. Those look fine.

As for syringe to agar, no. I tried it on multiple types of agar, it's just not as successful as PF tek with syringe. If anything buy a spore print not a syringe if you want to go straight to agar.


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Offlinespacechildo
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Re: flipped stalled brf jars, birthing [Re: robbinbanks]
    #19394657 - 01/09/14 05:05 PM (10 years, 21 days ago)

Quote:

robbinbanks said:
How are you gonna tell me to be patient when i didnt tell you the time frame yet? but, 3 and a half weeks, and you are probly right ha




3,5 weeks after inoc? That's not especially long, sometimes my jars take over 2 weeks just to germinate.
I just guessed you were impatient because you had flipped a jar that's just showing a coin-sized piece of growth.


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Offlinerobbinbanks
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Re: flipped stalled brf jars, birthing [Re: Camtaro420]
    #19394658 - 01/09/14 05:06 PM (10 years, 21 days ago)

kk...good to know! I am def considering a print next time. Any strain recommendations? I know some are more difficult than others


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Offlinerobbinbanks
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Re: flipped stalled brf jars, birthing [Re: spacechildo]
    #19394665 - 01/09/14 05:07 PM (10 years, 21 days ago)

that coin size one popped up like 2 weeks ago lol...i think it could be a contam :/


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OfflineCamtaro420
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Re: flipped stalled brf jars, birthing [Re: robbinbanks]
    #19394719 - 01/09/14 05:17 PM (10 years, 21 days ago)

Get us some more close up pics so we can tell. I gotta head out for now but i'll be back later.

As for the print. A cube is a cube. Pick one that looks good to you. I prefer the look of albino's myself.

Cya later bud.


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Offlinespacechildo
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Re: flipped stalled brf jars, birthing [Re: robbinbanks]
    #19394760 - 01/09/14 05:25 PM (10 years, 21 days ago)

Quote:

robbinbanks said:
kk...good to know! I am def considering a print next time. Any strain recommendations? I know somenone are more difficult than others




Just pick a name you like, a cube is a cube. There's a strain thread dedicated to the discussion of varieties of cubes if you are interested.


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Offlinerobbinbanks
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Re: flipped stalled brf jars, birthing [Re: Camtaro420]
    #19394762 - 01/09/14 05:25 PM (10 years, 21 days ago)

will do, thanks for your help! and albinos are def look legit:cool:


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Offlinerobbinbanks
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Re: flipped stalled brf jars, birthing [Re: spacechildo]
    #19394768 - 01/09/14 05:27 PM (10 years, 21 days ago)

kk, thanks space child..i will def have to do that!


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Offline1down5up
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Re: flipped stalled brf jars, birthing [Re: robbinbanks]
    #19394831 - 01/09/14 05:38 PM (10 years, 21 days ago)

You can only birth them one way...upside down...unless you have a zero gravity room...in which case, take lots of pics!!! lol

The taller jars (when uncontaminated) usually slow down when they get to the bottom of the jar because the sub compacts from all the movement.  I would never flip my jars, no matter what.  You should never disturb that dry verm layer...I'd rather take a piss in the GE holes than turn them upside down.  I'm not saying they won't contaminate with piss, i'm just saying.


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~~Everything is relative~~


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Offlinespacechildo
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Re: flipped stalled brf jars, birthing [Re: 1down5up]
    #19394900 - 01/09/14 05:48 PM (10 years, 21 days ago)

you can flip them, but only once.
it can help the bottom part colonize by giving it some more headroom.
they stall because water draws down by gravity, and gravity compressing the sub.


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