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bigd17
Say Budget To Me Again...



Registered: 07/31/08
Posts: 334
Loc: Dexter's Laboratory
Last seen: 3 years, 7 months
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multispore? please don't neck me...
#19391564 - 01/09/14 01:07 AM (10 years, 22 days ago) |
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Ok, so i feel like i'm going to get a lot of negative feedback regarding this being a stupid question but I cannot for the life of me find any posts containing a description to what multispore means.
is it different strains of the same species mixed together or is it something else?
-------------------- "The secret to success is to make your Vocation a Vacation." -Mark Twain Confucius say,"man who stand on toilet, high on pot." The answer to the question never asked is always no.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: multispore? please don't neck me... [Re: bigd17]
#19391567 - 01/09/14 01:09 AM (10 years, 22 days ago) |
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no it's just starting from spores and not an isolate
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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kratom_redmomd
Kratom Lover



Registered: 07/25/13
Posts: 411
Loc: Hiking Cascadia
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Re: multispore? please don't neck me... [Re: cronicr]
#19391620 - 01/09/14 01:27 AM (10 years, 22 days ago) |
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When starting from a print or syringe, your growth is not from a single spore, but from many many. That's where multispore (MS) comes from.
-------------------- "If the goal is to ride the bike, why is everyone arguing about which bike to ride? We are all bike riders. Ride your bike." -Unknown If I was going to destroy a nation, first I would destroy its language. -Confusious ¿Hablas Inglés?
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livingmagic
るんるん



Registered: 12/03/13
Posts: 3,015
Loc: Big Green Amore
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Re: multispore? please don't neck me... [Re: bigd17]
#19391645 - 01/09/14 01:33 AM (10 years, 22 days ago) |
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--------------------
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bigd17
Say Budget To Me Again...



Registered: 07/31/08
Posts: 334
Loc: Dexter's Laboratory
Last seen: 3 years, 7 months
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i had a strange feeling it was going to be that simple... well thank you for the quick response and for suffering my ignorance.
-------------------- "The secret to success is to make your Vocation a Vacation." -Mark Twain Confucius say,"man who stand on toilet, high on pot." The answer to the question never asked is always no.
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gornyhuy
A Myth Intrepidly Met



Registered: 08/04/09
Posts: 2,933
Loc: USA
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Re: multispore? please don't neck me... [Re: bigd17]
#19392532 - 01/09/14 07:35 AM (10 years, 22 days ago) |
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Damn, that is some awesome footage!
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tripmob
strang and unusual



Registered: 01/04/14
Posts: 176
Last seen: 7 years, 4 months
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Re: multispore? please don't neck me... [Re: gornyhuy]
#19392622 - 01/09/14 08:16 AM (10 years, 22 days ago) |
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Nice footage!...to the OP, your not the only one who found that information informative!...even the most trusted cultivators here needed help at one point!..I dont think its the noobie questions that aggravate people here, its the fucking stupid questions....like "what cube strain is best for beginners?" That tend to get on peoples nerves.
-------------------- Theres only 2 things I hate in life, Racism and Chinese People. 10, 000 rocks of crack cocaine is too much!
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bigd17
Say Budget To Me Again...



Registered: 07/31/08
Posts: 334
Loc: Dexter's Laboratory
Last seen: 3 years, 7 months
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Re: multispore? please don't neck me... [Re: tripmob]
#19393008 - 01/09/14 10:24 AM (10 years, 22 days ago) |
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haha I git you like if you used the search function would would see an ocean of "cubes is cubes" comments as far as the eye can see.
-------------------- "The secret to success is to make your Vocation a Vacation." -Mark Twain Confucius say,"man who stand on toilet, high on pot." The answer to the question never asked is always no.
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bigd17
Say Budget To Me Again...



Registered: 07/31/08
Posts: 334
Loc: Dexter's Laboratory
Last seen: 3 years, 7 months
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Re: multispore? please don't neck me... [Re: bigd17]
#19393020 - 01/09/14 10:27 AM (10 years, 22 days ago) |
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Also and I'm sure that this is in the advanced Mycology section but I'm assuming using a microscope and some agar, cultures can be started from a single spore... for the purpose of isolating potentially beneficial genetics??
-------------------- "The secret to success is to make your Vocation a Vacation." -Mark Twain Confucius say,"man who stand on toilet, high on pot." The answer to the question never asked is always no.
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tripmob
strang and unusual



Registered: 01/04/14
Posts: 176
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Re: multispore? please don't neck me... [Re: bigd17]
#19393154 - 01/09/14 11:06 AM (10 years, 22 days ago) |
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Im a noob myself. Im sure that you could start a culture with just a single spore, isolating a single lone spore seems to me would be very hard to do!...but I am deffinatly not qualified to answer that question.
-------------------- Theres only 2 things I hate in life, Racism and Chinese People. 10, 000 rocks of crack cocaine is too much!
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: multispore? please don't neck me... [Re: tripmob]
#19393162 - 01/09/14 11:08 AM (10 years, 22 days ago) |
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Quote:
tripmob said: Im sure that you could start a culture with just a single spore,
Nope.
2 maybe...not just one.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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gornyhuy
A Myth Intrepidly Met



Registered: 08/04/09
Posts: 2,933
Loc: USA
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Re: multispore? please don't neck me... [Re: bigd17]
#19393187 - 01/09/14 11:14 AM (10 years, 22 days ago) |
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Not exactly. The most common practice is to germinate mycelium from multi-spore on agar (or to clone and grow mycelium from mushroom tissue with desired characteristics) and then to transfer tiny samples of the most promising growth sectors from that plate to a fresh plate. You repeat that process until you have an isolate, which is basically the result of one isolated set of genetics. Then you grow that out.
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tripmob
strang and unusual



Registered: 01/04/14
Posts: 176
Last seen: 7 years, 4 months
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Re: multispore? please don't neck me... [Re: PussyFart]
#19393194 - 01/09/14 11:16 AM (10 years, 22 days ago) |
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Bah, damn. My logic was, in the wild a single spore can reproduce(at least according to some cool video I just watched) figures I would be wrong! On the bright side, next time I answer this question, ill be right!
-------------------- Theres only 2 things I hate in life, Racism and Chinese People. 10, 000 rocks of crack cocaine is too much!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: multispore? please don't neck me... [Re: tripmob]
#19393232 - 01/09/14 11:21 AM (10 years, 22 days ago) |
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Quote:
tripmob said: Bah, damn. My logic was, in the wild a single spore can reproduce(at least according to some cool video I just watched) figures I would be wrong! On the bright side, next time I answer this question, ill be right! 
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bigd17
Say Budget To Me Again...



Registered: 07/31/08
Posts: 334
Loc: Dexter's Laboratory
Last seen: 3 years, 7 months
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Re: multispore? please don't neck me... [Re: cronicr]
#19393552 - 01/09/14 12:34 PM (10 years, 21 days ago) |
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Oh right on when put that way isolating sounds a bit simpler than listening to the vets discuss it in mycogreek lol.
-------------------- "The secret to success is to make your Vocation a Vacation." -Mark Twain Confucius say,"man who stand on toilet, high on pot." The answer to the question never asked is always no.
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gornyhuy
A Myth Intrepidly Met



Registered: 08/04/09
Posts: 2,933
Loc: USA
Last seen: 2 years, 2 months
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Re: multispore? please don't neck me... [Re: bigd17]
#19393736 - 01/09/14 01:33 PM (10 years, 21 days ago) |
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Yeah, it takes a lot of patience and good judgement on which sectors to pick, and especially good sterile practices... but aside from that its not hard, and its very cool and rewarding.
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 2 months
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Re: multispore? please don't neck me... [Re: gornyhuy]
#19393915 - 01/09/14 02:21 PM (10 years, 21 days ago) |
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I'll probably get fuckin' flogged for this, but i find multispore a bit of a misnomer. You could easily just say "spores" and mean the exact same thing, without the confusion. "i knocked them up with spores", and "I knocked them up with MS" mean the exact same thing. If you use spores, then obviously, you must be using multiples of them. When i first heard multispore, i thought...
"wtf...are they using spores from multiple mushrooms? Where the fuck do i get those?"
After banging my head, and feeling like an idiot, i realised that it basically means multiple spores....but if you use spores at all, you must use more than one....good luck isolating one spore from the millions....and good luck growing anything with it. I guess it's just an accepted term, and will always stay that way, but i can see how you got confused.
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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bigd17
Say Budget To Me Again...



Registered: 07/31/08
Posts: 334
Loc: Dexter's Laboratory
Last seen: 3 years, 7 months
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Re: multispore? please don't neck me... [Re: 1down5up]
#19396745 - 01/10/14 12:11 AM (10 years, 21 days ago) |
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yeah dude i been wondering what the hell that meant for quite awhile now i just didnt have reason to ask as i'm still really a beginner only now working on what i hope to be my first successful grow, i always figured multispore was an advanced term referring to some other process and thus figured i'd find out when i got more experience.
-------------------- "The secret to success is to make your Vocation a Vacation." -Mark Twain Confucius say,"man who stand on toilet, high on pot." The answer to the question never asked is always no.
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