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Mo_Mo
Stranger

Registered: 12/08/13
Posts: 8
Last seen: 1 year, 4 months
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Re: First Grow (Log kinda) [Re: NitroCat]
#19407027 - 01/12/14 05:57 AM (10 years, 19 days ago) |
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Love watching the progression.
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NitroCat
HNO3, H2SO4, Cat



Registered: 12/28/13
Posts: 64
Last seen: 1 year, 8 months
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Re: First Grow (Log kinda) [Re: Mo_Mo]
#19407093 - 01/12/14 06:39 AM (10 years, 19 days ago) |
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It really is. So far, of the jars I've Steamed/PC'd, 6 or so were rejected outright (flooded with water), and all but two are showing growth (Of the batch that's had time to, at least!). Those that are not showing any growth are in jars that we're cocked up from the get go.. Accidently the air exchange hole with solid silicone, which does admittedly make me wonder if placing a backing on either/both side(s) of the injection ports could make it more stable to use
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NitroCat
HNO3, H2SO4, Cat



Registered: 12/28/13
Posts: 64
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Re: First Grow (Log kinda) [Re: NitroCat]
#19412997 - 01/13/14 03:46 PM (10 years, 17 days ago) |
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I do have a couple questions though, namely just wondering how other's would go about fruiting these cakes. I have a ton of verm and a few bricks of coir, ad well as a large tub that's been tentatively reserved for a SGFC. Basically just debating if I should dunk-roll them all, or if I want to make the effort in casing. Considering I have a few cakes I'm leaning towards the option of "Both"
But either way, anyone have a suggestion for what to do with the packed-cake? I wasn't planning on rushing it out to birth based on surface colonization, just because it'd take at least as long as the rest all said and done to consolidate.
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NitroCat
HNO3, H2SO4, Cat



Registered: 12/28/13
Posts: 64
Last seen: 1 year, 8 months
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Re: First Grow (Log kinda) [Re: NitroCat]
#19415828 - 01/14/14 04:42 AM (10 years, 17 days ago) |
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Popping back in because I have something a little odd. I checked my new cakes tonight and noticed some large, purple-black dots and splotches near sites of what might be potential growth


One thing I did notice and am extracting most likely vain hope from is the splotting is all in a path where material would land during innoc, and the syringe uses was very, very black-purple with what I'd hoped to be spores. Of the jars in here, only two of the three done with this spore syringe are showing the discoloraition. Nine were done in total this batch, with each set of three on it's own supplied syringe and needle, which should help keep cross contamination down, and none of the other provided samples have shown the same discolouration.
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xBELLSPROUTx
Pokemon Master



Registered: 12/27/13
Posts: 448
Last seen: 3 years, 6 days
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Re: First Grow (Log kinda) [Re: NitroCat]
#19415966 - 01/14/14 05:47 AM (10 years, 17 days ago) |
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It could definitely be a cluster of spores or It could also be a part of in-colonized verm or something, so of the verm has black stuff and other organic materials. When I did mine I had the same black spec now it's my jar with the most growth.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: First Grow (Log kinda) [Re: NitroCat]
#19415985 - 01/14/14 05:58 AM (10 years, 17 days ago) |
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I see no dry verm barrier. and those pics looks pretty wet to me.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
spacechildo said: I see no dry verm barrier. and those pics looks pretty wet to me.
OP is using ship and tyvek. Still I agree that it looks quite wet.
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NitroCat
HNO3, H2SO4, Cat



Registered: 12/28/13
Posts: 64
Last seen: 1 year, 8 months
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Re: First Grow (Log kinda) [Re: Pastywhyte]
#19417704 - 01/14/14 02:53 PM (10 years, 16 days ago) |
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Lack of dry verm is intentional. I'll agree they're a bit wet, there was probably some unintended water penetration while they were PCing, and a few of the jars just ended up abnormally wet. I didn't get any good pictures of it, but the splottyness was pretty clearly not in the substrate. It kinda clung to where the fluid had dripped. Thanks for the assurance on it! The older jars are all going pretty happyly so far, might try and snag some pics when I get home tonight
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NitroCat
HNO3, H2SO4, Cat



Registered: 12/28/13
Posts: 64
Last seen: 1 year, 8 months
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Re: First Grow (Log kinda) [Re: NitroCat]
#19429287 - 01/16/14 05:25 PM (10 years, 14 days ago) |
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Fresh pictures! Triple pyramid, all the jars actively showing what looks like strong, healthy growth. In the tall jars; On the left - Oak Ridge On the right - B+

And the... Box of Shame. In this box are all innoc'd jars which are showing minimal at best growth

While I want to blame anything but the spores here, we have 5 jars (Of -six-) all of the same (sub)variety, and they do seem to be lagging. Guess that syringe wasn't a particularly good ticket for the genetic lottery.
On the note of comparing varieties, I'm at a loss for a *objective* measure of potency. The only viable solution I've come to would be to attempt a solvent extract and then triturate it with something that'd precipitate it as a insoluble salt... Of course, I have no idea how to approach the extraction at the moment in a way that'd at least preserve a useful ratio (Anything used for testing in this way will most likely be inedible.) and can't think of a good compound to attempt the reduction. The only reason I'm not going with "Just eat them" as a measurement of potency is that is entirely too variable to make a objective test, setting, mood and tolerance can all make a one milligram dose (Lets just pretend that is the quantity of active compounds in our hypothetical dose of dried mushroom)seem dramatically more or less potent.
Of course, any of that will take some dedicated, most likely very expensive equipment (A good buret alone will cost me more then my current and planned near-future grow setup) and sourcing some of it will most likely be a utter pain. All of that assuming that I can even find or have the needed skill to chemically analyze the samples, putting it off in the "Fun, but probably not happening soon" category of ideas.
Probably going to do one 'last' full batch of cakes this weekend, or be grabbing some WBS (Or both ). If it's cakes, it'll probably be a 2 jar pre (sub)variety race of all six I have on hand, if anyone would be interested in such a thing. Weighed out substrate and probably all mixed in one big batch to help remove variables from the system.
Ninja edit! Leaning towards stuffing the cake in some sort of casing/tub when that time comes. The tub I'd intended to use for a SGFC is big enough it's really calling to be to become a SAB, to help it embrace... Destiny. :V Sorry little sick and hydrophone makes me weirder
Edited by NitroCat (01/16/14 06:07 PM)
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NitroCat
HNO3, H2SO4, Cat



Registered: 12/28/13
Posts: 64
Last seen: 1 year, 8 months
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Re: First Grow (Log kinda) [Re: NitroCat]
#19433373 - 01/17/14 02:01 PM (10 years, 13 days ago) |
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Hahah... Well, looks like I may have a contaminate issue; One of the Eq jars has a handful of menacing looking black pins in it that really do not healthy, the jar is also nowhere near 100%. Will try and snag a good pic or two this weekend.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: First Grow (Log kinda) [Re: NitroCat]
#19433385 - 01/17/14 02:06 PM (10 years, 13 days ago) |
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that sux nuts
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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NitroCat
HNO3, H2SO4, Cat



Registered: 12/28/13
Posts: 64
Last seen: 1 year, 8 months
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Re: First Grow (Log kinda) [Re: NitroCat]
#19433787 - 01/17/14 03:36 PM (10 years, 13 days ago) |
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It is what it is, I probably got a few filters wet and that's where I'd put the "biting me in the ass" money :V The potential contaim jar is gonna make me bang out some WBS and whatever else I can over the weekend though. If a hole saw throws itself at me I'll get my SAB set up.
Hey, at least the fuck-ups doing this don't want to try and eat (explode) my fingers, so it's all fine so far <,,<
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xBELLSPROUTx
Pokemon Master



Registered: 12/27/13
Posts: 448
Last seen: 3 years, 6 days
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Re: First Grow (Log kinda) [Re: NitroCat]
#19434176 - 01/17/14 05:19 PM (10 years, 13 days ago) |
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Make sure the black pins aren't a cluster of spores before anything rash is done.
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NitroCat
HNO3, H2SO4, Cat



Registered: 12/28/13
Posts: 64
Last seen: 1 year, 8 months
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I'm letting it go for a bit, the jar in question has been separated from the rest. It's on one of the first-generation jars and is very clearly new growth. The clearest one last night was a black dot about... 1/16th" in diameter and clearly connected to the myc, with a few more pins scattered in the jar, a few of them in a cluster at the bottom-curve.
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xBELLSPROUTx
Pokemon Master



Registered: 12/27/13
Posts: 448
Last seen: 3 years, 6 days
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Re: First Grow (Log kinda) [Re: NitroCat]
#19434419 - 01/17/14 06:06 PM (10 years, 13 days ago) |
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does it look like
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NitroCat
HNO3, H2SO4, Cat



Registered: 12/28/13
Posts: 64
Last seen: 1 year, 8 months
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No, I had a couple of those in a new jar. It's very clearly growing up from the jar. Asking my assisting friend to snap a picture. They're not trapped at work at the moment and reported that the pins are apparently multiplying
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xBELLSPROUTx
Pokemon Master



Registered: 12/27/13
Posts: 448
Last seen: 3 years, 6 days
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Re: First Grow (Log kinda) [Re: NitroCat]
#19434513 - 01/17/14 06:34 PM (10 years, 13 days ago) |
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Umm, sounds like a contamination :/ sorry man, I would deff stick it out and see how it goes.
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NitroCat
HNO3, H2SO4, Cat



Registered: 12/28/13
Posts: 64
Last seen: 1 year, 8 months
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Re: First Grow (Log kinda) [Re: NitroCat]
#19434648 - 01/17/14 07:12 PM (10 years, 13 days ago) |
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Kinda figured it was last night. It thankfully was the one and only jar showing them last I looked, and it was lagging behind the rest in terms of colonization anyway. Live and learn and all that. Also hole saws are stunningly cheap, so odds are I'm going to try and get the SAB done tomorrow and knock another batch or two up.
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xBELLSPROUTx
Pokemon Master



Registered: 12/27/13
Posts: 448
Last seen: 3 years, 6 days
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Re: First Grow (Log kinda) [Re: NitroCat]
#19434735 - 01/17/14 07:30 PM (10 years, 13 days ago) |
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it could just be a bunch of clusters so dont count your eggs before they hatch, nature works in mysterious ways. keep us updated and send those pics in so we can look.
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NitroCat
HNO3, H2SO4, Cat



Registered: 12/28/13
Posts: 64
Last seen: 1 year, 8 months
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Re: First Grow (Log kinda) [Re: NitroCat]
#19441247 - 01/19/14 05:48 AM (10 years, 12 days ago) |
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Cake in question, couldn't manage a good picture until today >_> Feeling a bit better about it now, really. Looking back at these first few cakes, oh god were my substrates waaaay over hydrated. D:
Haven't gone out and gotten grain yet, but did start a few more BRF jars tonight. Almost out of BRF so I'll be discontinuing them sooner than later <,,< Like say, tomorrow. As long as I don't sleep through the entire damned day again :V New jars should be a looot better preforming than their counter parts of the last couple generations.
They're in the wide and short half-pints rather then the tall/widemouthed ones of the last batch. The air filter hole's been expanded to a full 3/4" compared to the 1/4 of the last few, and is siliconed on much, much, much cleaner with better airflow.
Will be knocking them up tomorrow sometime, throwing two of each variety I have on hand out into the field, expect slightly more analytical notetaking hopefully~
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