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Watamiseh
Stranger


Registered: 12/04/13
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Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q'
#19389466 - 01/08/14 05:23 PM (10 years, 22 days ago) |
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35.6 gallon tub that I messed up. Got 5" of perlite.
Stupidly used a 3/8" drill tip instead of 1/4". Spaced every 2". (Things always mess up when excited/in a hurry)

Anyone have suggestions? Next in mind was to make a "one row taped, one open" all around but I guess it's futile not knowing if the hygro is way off or not.
Tried putting saran wrap over 3-4 rows of holes all around and it was giving readings of 94% RH and when I completely unwrap it open it, it hovers at 80%'s (according to cheap hygro)
I've got a few trays/cakes/logs I'd like to experiment with (currently 50% colonized): -2 mini straw logs (2.5" depth, 8" length kinda brick shaped) -Straw tray(11" length x 8" width & 2" sub depth) -2 bar shaped small coco coir trays(2" depth, 6" length x 2" width) -2 PF cakes that I fruited one flush in bottles, 16 hr dunked and going another flush in this sgfc (so far some new pins are forming) (I had in mind to
I've got an 80qt backup tub that I'll turn into a sgfc too just in case.
On another note:
I'm going to buy this hygrometer it costs $40. Anyone familiar with this make? Just waiting till I get the money. "synthetic hair hygrometer" as I've seen others recommend.

First time grower, my first flushes from my pf cake bottles gave me an amazing first trip at 25g fresh and now definately in love! 
Would appreciate any ideas on how to make this work.
Edited by Watamiseh (01/08/14 05:27 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19389680 - 01/08/14 05:56 PM (10 years, 22 days ago) |
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well noramaly hygro's are not needed for sgfc's, worse case scenario you gotta mist more often
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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VE3HPC
Technician


Registered: 11/30/13
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19389697 - 01/08/14 05:58 PM (10 years, 22 days ago) |
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If you are trying to bring more humidity back to the chamber, why not just take some high quality duct tape or gorilla tape and cut neat squares/circles to patch a few of the holes. Patch like 20 to 30 holes, wait 12 hours, take a reading on the hygrometer. If better reading but still not ideal conditions, patch a few more. Also the digital hygrometers are usually not as accurate as analog versions, just due to the possibility of water vapor condensing on the sensor while at the higher humidity ranges. Analog gauges are slightly more forgiving in this respect, leading to a more accurate reading. Good luck, I wouldn't throw that tub out just because of hole size! That tub actually looks pretty good! I would use it
-------------------- -"It's all been done before. You're just another brain going through another depression. Accept it, treat it, move on."
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baldwintpenguin
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19389711 - 01/08/14 06:00 PM (10 years, 22 days ago) |
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Im sure someone on here might disagree with this....but.....just tape up some holes.....Ive used just regular clear packing tape and taped up some, start with maybe 1/8 to 1/4 of your holes and see how it effects humidity and adjust as needed
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 "There he goes. One of God's own prototypes. A high-powered mutant of some kind, never even considered for mass production. Too weird to live, and too rare to die."
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NOTFALL3N
Epic

Registered: 11/18/13
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: baldwintpenguin]
#19389752 - 01/08/14 06:06 PM (10 years, 22 days ago) |
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It will be fine mist and fan more often I'll be fine don't worry!
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: NOTFALL3N]
#19389834 - 01/08/14 06:19 PM (10 years, 22 days ago) |
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3/8 inch holes actually work better than 1/4 holes you just have to mist more but it's more optimal.
Don't listen to these other guys, Taping holes will lower your humidity and fuck your FAE the two things that mushrooms don't want you to do.
Here's my write up on the function of a SGFC, I hope it helps you understand how they work.
Quote:
Fruiting Chamber A SGFC has 1/4" holes spaces 2 inches apart in a grid patten on all six sides. 4-6 inches of moist perlite. No attachments made to it with any extra things like humidifiers. Optimally the SGFC should be in the middle of a room. No fans should be run in the room with the SGFC, but a cracked window is OK. A humidifier in your house can help to raise the ambient RH but don't put it near your SGFC put it in the other corner of the room if you do decide to run a humidifier at all. The SGFC IMO should have at least 6-12 inches of room from any wall on all 6 sides. This includes finding some sort of raisers to elevate the SGFC off of the surface it's on.
Misting and Fanning. This is a source of much grief and 100's of posts a week here. Misting and fanning is not at all complicated as it needs to be. In general you'll mist your cakes until they glisten(yes they can even with the verm on them) and then fan right after the mist. You can mist your cakes directly and you should. When you notice the cake is no longer glistening you can mist it again and then fan. This occurs on average of 3-5 times a day. Don't worry about sleeping or being gone 12 hours. Just do it when you're around and don't forget about it is all. Fanning is not FAE it's only purpose is to relive the high RH air so that the cakes can get a kickstart on evaporation.
FAE This is a phenomenon (Fresh air exchange) in a properly built SGFC this is constantly happening. The perlite is naturally cooler than the surrounding air this moves molecules closer to eachother as the lose kinetic energy. This creates low pressure which pulls air up through the bottom holes. As the air moves through the perlite it picks up humidity and keeps the chamber at or above 90%RH. This occurs naturally without the fanning and is why we like to have no fans in the room and is also why fanning after misting is not a replacement for FAE.
Quote:
Notahacker420 said: Fanning is not a replacement for constant FAE.
You would need to fan several times per hour.

Edited by Trusted cuItivator (01/08/14 06:21 PM)
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: bodhisatta]
#19389854 - 01/08/14 06:23 PM (10 years, 22 days ago) |
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As for humidity gauges they're all garbage you need a good expensive one made by fisher scientific in Germany or an analog one for cigar boxes you can calibrate otherwise the readings you're getting are just some numbers.
Quote:
RogerRabbit said: I experimented with every hole size possible before settling on 1/4" (6mm). Best performance was with 3/8" holes, but you have to mist more. Since most people are lazy I settled on the 1/4" size because in most cases the terrarium can be handled with a misting or two per day. RR
Quote:
A quick search will reveal that when I designed the shotgun terrarium I was working 12 hour shifts, an hour drive from home. This resulted in being gone 14 hours per day, with no way to perform fanning or misting.
A double outlet 100 gallon aquarium pump in a shotgun terrarium will result in about ten percent of the air exchange you would get naturally, plus destroy the flow of air up through the perlite, causing all sorts of problems.
Hollow stems and poor potency are not FAE or humidity related, so instead of fixing what isn't broken, lower the temp to prevent hollow stems and isolate proper genetics to get the potency you desire. You will never get consistent potency when growing from spores. Every grow is like a roll of the dice. RR
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: bodhisatta]
#19389865 - 01/08/14 06:25 PM (10 years, 22 days ago) |
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Quote:
bodhisatta said: 3/8 inch holes actually work better than 1/4 holes you just have to mist more but it's more optimal.
Don't listen to these other guys,
whatcha talking about! thats exactly what i told him

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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: bodhisatta]
#19389869 - 01/08/14 06:26 PM (10 years, 22 days ago) |
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I'm going to buy this hygrometer it costs $40. Anyone familiar with this make? Just waiting till I get the money. "synthetic hair hygrometer" as I've seen others recommend.
you're right that that's a better choice than most for hygrometers(the synthetic hair kind) but 40 is pretty cheap. Fischer makes a good one but I'm sure they're more than 50$ easily. Maybe that one is good , but you don't need it anyway.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: cronicr]
#19389878 - 01/08/14 06:28 PM (10 years, 22 days ago) |
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Quote:
cronicr said:
Quote:
bodhisatta said: 3/8 inch holes actually work better than 1/4 holes you just have to mist more but it's more optimal.
Don't listen to these other guys,
whatcha talking about! thats exactly what i told him

I was considering quoting them and pointing them out but I know how people get super butthurt leave 0/5 ratings and bomb your inbox for a week about things done for the common good.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: baldwintpenguin]
#19389883 - 01/08/14 06:29 PM (10 years, 22 days ago) |
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oh i know all too well lol and so did this guy..he expected itQuote:
baldwintpenguin said: Im sure someone on here might disagree with this....
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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NOTFALL3N
Epic

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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: cronicr]
#19390173 - 01/08/14 07:39 PM (10 years, 22 days ago) |
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Lol
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Watamiseh
Stranger


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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: NOTFALL3N]
#19390512 - 01/08/14 08:39 PM (10 years, 22 days ago) |
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Awesome!
Gonna leave it as is and just mist a lot. Heard people mist their sgfc's 1-2x a day so I'd be going for 4x? Guess If looking at the positives FAE and cleanliness is sorted with this setup.
I bet these straw cakes will devour the air... they look like voracious lil f****s. Mycelium is Tearing through the straw and it's only been 2 days!


Since the FC is so large(lots of room for CO2 to dissipate) I could probably get away with taping over a few holes some time down the track if it ends up being problematic
I thought "Dammit, RR can run his sgfc with the lid off and still have 90+ RH while mine doesn't cause of hole size? "
The weather here is always 60% humidity on average as well.
"Wetterladen Klimatest 45.2030.50 Synthetic Hair Precision Hygrometer Anthracite"
was the one I had in mind but I'll definately look into tobacco hygros as well for other projects and just to check if things are okay
Cheers lads!
Edited by Watamiseh (01/08/14 08:46 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19390564 - 01/08/14 08:50 PM (10 years, 22 days ago) |
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mist when your surface looks dry, theres no real schedule;)
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
Edited by cronicr (01/08/14 08:52 PM)
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Watamiseh
Stranger


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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: cronicr]
#19461045 - 01/23/14 05:47 AM (10 years, 8 days ago) |
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Update!
now that I've put a whole bunch of stuff in the SGFC its reading 90%+ on the digital hygrometer. on a rainy day it reads as 96%-99% and now on regular days 86%-92%ish
I'm amazed at how HUGE these mushies are already on straw compared to BRF.

Officially my 2nd grow and 1st for straw! So far I'm so in love with these shrooms I want to hug them 
3 mistings a day seem to do it good but I don't fan since i figured this SGFC has massive FAE.
PS these mini straw logs/cakes are propped up on coke cans.
edit: just for shits and giggles I tried a crappy $1 analogue hygrometer in there and it doesn't even go above 80%
Edited by Watamiseh (01/23/14 05:53 AM)
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monoculture
Mycaddict



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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19461159 - 01/23/14 07:04 AM (10 years, 8 days ago) |
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With the SGFC that full, be happy you made the holes bigger. The more you put in, the more co2 is produced. The humidity will be high enough, especially when mushrooms are growing.
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PussyFart
Retired Cultivation Extrodinaire



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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: monoculture]
#19461215 - 01/23/14 07:25 AM (10 years, 8 days ago) |
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Quote:
monoculture said: With the SGFC that full, be happy you made the holes bigger.

Quote:
monoculture said: The more you put in, the more co2 is produced.
And the bigger the tub the better the air circulation and exchange of gasses.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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monoculture
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: PussyFart]
#19461257 - 01/23/14 07:38 AM (10 years, 8 days ago) |
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: monoculture]
#19461666 - 01/23/14 09:40 AM (10 years, 7 days ago) |
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Quote:
3 mistings a day seem to do it good but I don't fan since i figured this SGFC has massive FAE.
the fanning is just to remove the mist particles from the air so that the mist that landed on your substrates can evaporate into the relatively dry air. I would still fan after the mist unless you bring you substrate out of the SGFC mist it and then put it back in.
Also RR has mentioned the SGFC works best with 3/8" holes but it requires more maintenance in the misting dept and rehydrating the perlite. He wanted it to be quite maintenance free and low hassle so settled with 1/4" holes which still perform well.
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vikingsc
Mushroom Enthusiast



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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: bodhisatta]
#19462281 - 01/23/14 12:13 PM (10 years, 7 days ago) |
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Nice. So the FC is working well as is? Are you having any trouble maintaining humidity or drying of sub?
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Jeeter
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: vikingsc]
#19462325 - 01/23/14 12:23 PM (10 years, 7 days ago) |
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My digital hygro never creeps above 65 but I know with the water droplets on the walls, I'm way over that. Just mist and then Fan like a mad man. End result is always Bomb-diggity tho. The term "Fruits of your labor" comes to mind.
Patience, attention to detail, and good ol fashion hardwork.
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vikingsc
Mushroom Enthusiast



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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Jeeter]
#19462724 - 01/23/14 01:55 PM (10 years, 7 days ago) |
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Ya the cheap hygro's suck. If the sides of the FC stay moist then your humidity should be fine.
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Watamiseh
Stranger


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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: vikingsc]
#19462976 - 01/23/14 02:52 PM (10 years, 7 days ago) |
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Depending on the weather(temperature/humidity etc) it gets a bit harder to keep the perlite hydrated. When the weather is warm there's not much condensation so it's hard to tell if the humidity is right apart from putting faith in the hygrometer. There's a lot of clutter around the fc atm and it might interfere with the circulation so I'm kind of overzealous and constantly misting the walls of the fc just to kind of guarantee the air is more humid in there.
To be honest I can't tell when the sub is drying or not since I don't have a good feel for it yet. Straw is a bit tricky looking too. The straw was possibly over-hydrated initially which seemed to make it hard for the mycelium to colonize the really wet parts (they were sweating like crazy).
I was worried about losing my babies to some kinda rot or bacteria etc So I took them out of their bags and let them air out a bit since they were completely colonised, but just needed consolidation. There was a sort of dirty smell which subsided when I let the substrate air out a bit). They did lose some water and the outside was able to colonise more thoroughly after that.
Within 2-3 days the surface was pinning so I thought I'd just fruit them since they were happy to do so. The straw got birthed by getting a strong misting for a day till the surface was swamped - then let it evaporate off and begin regular light misting the fc and perlite.
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Watamiseh
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19462981 - 01/23/14 02:53 PM (10 years, 7 days ago) |
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These mushrooms look really healthy. But I will start fanning now since not all the little knots have pinned yet.
My BRF cakes had really thin blonde looking shrooms with relatively proportionate caps but these straw ones look really meaty, dark and strong. I'm gonna let this 4 inch shrummie's veil break and try clone it after I take a spore print from it (it still seems to be growing).
Will be printing it on a petri dish, I bought a box of 500 to fool around with. (I've never done any agar work besides the stuff I learnt at college/high school but not with shrooms - but my aseptic technique was pretty decent)
Kinda lazy about making some agar dishes but it has to be done!
I'm guessing I'd make a grain jar and throw a wedge of isolated agar in it to inoculate it. then maybe shake up the master jar with some sterile water and start inoculating a few more grain jars with it. (I've got some pretty badass glass syringes from china, got one that can hold 120ml)
The legacy of straw shrummies must live on!
(I ramble on too much so I gotta split my posts )
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Watamiseh
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: monoculture]
#19474801 - 01/26/14 12:22 AM (10 years, 5 days ago) |
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Quote:
monoculture said: With the SGFC that full, be happy you made the holes bigger. The more you put in, the more co2 is produced. The humidity will be high enough, especially when mushrooms are growing.
I dunno, look at how closed off monotubs are compared to something like a SGFC. I probably have 1/5th the substrate volume in this SGFC than an equivalent monotub and look at all the open air holes this has. Even got the little modestly sized cakes/bricks propped up on coke cans to create a bit of circulation around em.
I guess these could of had more thorough pinning than I currently have. (the surface seems to be covered in knots but they haven't been pinning uniformly)
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monoculture
Mycaddict



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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19478718 - 01/26/14 08:15 PM (10 years, 4 days ago) |
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Quote:
I probably have 1/5th the substrate volume in this SGFC than an equivalent monotub
Ok, while looking at this pic:

I had the idea the SGFC was packed with 3 rows of bricks of decent size.
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vikingsc
Mushroom Enthusiast



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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19479124 - 01/26/14 09:50 PM (10 years, 4 days ago) |
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Quote:
monoculture said:
I guess these could of had more thorough pinning than I currently have. (the surface seems to be covered in knots but they haven't been pinning uniformly)
Likely from multi-spore genetics. The pics you see of pins everywhere across the tub and a canopy that takes up the whole tub are usually from an isolate or a clone.
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Watamiseh
Stranger


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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: vikingsc]
#19480037 - 01/27/14 03:53 AM (10 years, 4 days ago) |
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Oh that makes a lot of sense. I never knew!
Kept wondering why I'd get so many different looking shrooms too.
Trying my best to get agar work under way and hopefully get a mono culture. Still awaiting my clone attempts to colonize at the moment (I've done 3 tissue samples from the inner stems on barley malt agar plates).
Will post pics when done, or maybe start up a grow log to document my journey if irrelevant to this thread.
Love ya all! 
*edit* re:monoculture; pardon my bad photography
Edited by Watamiseh (01/27/14 03:57 AM)
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twistedty
Forcefully Retired



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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19480048 - 01/27/14 03:58 AM (10 years, 4 days ago) |
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looks like a shitty pinset due to a shitty enviroment
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Watamiseh
Stranger


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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: twistedty]
#19480113 - 01/27/14 05:23 AM (10 years, 4 days ago) |
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Hopefully my monotub won't have shitty conditions. I guess my pinsets represent the fruits of my labor. Maybe I need to put in more effort.
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blindingleaf
blue collar underworld



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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19480167 - 01/27/14 06:05 AM (10 years, 4 days ago) |
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good job man! if this is ur first/one of ur first attempts i salute u for trying straw! i've only used it for oysters. how did u prep it? soak, lime, pasteurize? perhaps if u left bag on the whole time (even consolidated in the bag because these are "cakes") you would have seen larger fruits/larger pin set both because more moisture would have been retained by bag holding it in and the myc having a chance to consolidate the nutrients before spending energy trying to fruit. sry if this was mentioned in the OP (i can only read 2nd page in middle of replying), but how did u inoculate the straw? did u spawn to it? i guess u'd have to..never head of ms or lc taking to straw like that. i feel like striping the bag may have done more harm than good (i know why u did it, i get paranoid too with smells/stalls haha) but oysters typically grown on straw logs require way more fae than cubes, and despite that, commercial growers don't even strip the bags, (in an effort to retain moisture) they poke holes or x's kinda like an SGFC, even though stripping the bags would increase FAE. so with cubes, it may have dried out the straw log a bit much to get a great pin set. maybe ur next flush will have more/larger fruits...? sry for the long response....i've been wanting to try straw with cubes for a while. thx for posting!
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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RogerRabbit
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19480233 - 01/27/14 06:58 AM (10 years, 4 days ago) |
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Quote:
Watamiseh said:
I'm gonna let this 4 inch shrummie's veil break and try clone it after I take a spore print from it (it still seems to be growing).
Very bad idea if you want good performance later. Clone from small, rapidly growing mushrooms, not a mature, open cap mushroom which has already entered senescence.
Quote:
Watamiseh said: Depending on the weather(temperature/humidity etc) it gets a bit harder to keep the perlite hydrated. When the weather is warm there's not much condensation so it's hard to tell if the humidity is right apart from putting faith in the hygrometer.
Condensation isn't an indicator of humidity.
There should never, and I repeat never be condensation on the walls of a shotgun terrarium. The only way that could ever happen is to put a heater inside and put the terrarium outdoors or something else stupid like that. There should never be condensation on a shotgun terrarium indoors. Condensation is not an indication of humidity; it's an indication of temperature differential. RR
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Watamiseh
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: blindingleaf]
#19480425 - 01/27/14 08:41 AM (10 years, 4 days ago) |
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blindingleaf, thanks dude! To be precise I followed the man RR's "Let's grow mushrooms" straw tek. So far the only educational material I needed to purchase. My first grow was 2 pf cakes fruited in coke bottles , I got 2 flushes out of them then I built the SGFC and got a few more cubies then the cakes just stalled (ran outta food I guess)
Cut up straw into 1-2" pieces, dish soap wash and soak in hot tap water for 2 hrs, then pasteurized for 90 minutes. I dunno what the etiquette is about sharing stuff from purchased material so forgive me if it's impolite in any way RR.
edit: I used 1:5 ratio of rye spawn to straw approx.
-2x 50/50 straw/cowpoo monotubs got trich. -2x coir mini trays doing ok (fast growing short stubby mushrooms) -straw tray-cake got trich after 1 flush -2x straw bag-cakes survived first flush, the second flush had an ok amount of pins but they built up a huge amount of fuzzy mycelium around the pins almost engulfing them this time. I misted them till drenched as a dunking substitute just in case. They definitely performed the best.
Just tried nearly everything at once for my first time. I'm practically broke but I feel like its worth it regardless.
I've bought some shiitake and oyster spore prints to try very soon when I free up the SGFC and use it for attempting my edibles on PF Tek till I get a feel for how they behave then move on to straw immediately after too.
----------------- ***RogerRabbit*** ----------------- Thank you very much for those tips, It was perfect timing for me to learn that before starting to store prints and cultures 
Made a lot of light spore prints on sterile petri dishes (I bought a box of 500 for $20 because they were slightly defective) so I use them generously.
Have around 10 malt extract agar plates in the fridge at the moment so I have a lot of experimenting to do. Never done agar work before but I did my best to follow aseptic procedures to minimize contams.
Would mixing a tiny bit of spores from my : Cambodian, Ecuador and golden teachers (all prints) in one agar dish be a good way to try select a strong colony of rhizo strands? Still doing my best to understand how the DNA mixes etc!
Edited by Watamiseh (01/27/14 08:45 AM)
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bodhisatta 
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19480704 - 01/27/14 10:36 AM (10 years, 3 days ago) |
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Quote:
Made a lot of light spore prints on sterile petri dishes (I bought a box of 500 for $20 because they were slightly defective) so I use them generously.
Have around 10 malt extract agar plates in the fridge at the moment so I have a lot of experimenting to do. Never done agar work before but I did my best to follow aseptic procedures to minimize contams.
Would mixing a tiny bit of spores from my : Cambodian, Ecuador and golden teachers (all prints) in one agar dish be a good way to try select a strong colony of rhizo strands? Still doing my best to understand how the DNA mixes etc!
the dishes were still guaranteed sterile?
aseptic wont help you, you need to be sterile. You need a Still air box and a good understanding of sterility, Technical Educational Knowledge=TEK good sterile TEK is what you need. type SAB and sterile into the search engine and tick the trusted cultivator box make posts newer than 2 years old.
mixing spores does nothing they're all p.cube spores. Two spores mate make dikaryotic(sp) mycelium that's a single strain. Using spores to start a grow ensures you have 1000s of strains. doesn't matter if the spores come from two corners of the world as long as they're p.cube its going to grow. rhizo growth happens on petri dishes after a few transfers and if you have your nutrient level right, rhizo growth likes low nutrient levels on agar. Don't worry about rhizo growth so much worry about healthy growth and fast growth.
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blindingleaf
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: bodhisatta]
#19481987 - 01/27/14 03:40 PM (10 years, 3 days ago) |
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im gonna try straw with cubes and no hydrated lime them (i usually add lime to pasteurization water for oysters). I'm glad it worked for you!
and for the agar, (bodhi i mean)
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Watamiseh
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: bodhisatta]
#19484472 - 01/28/14 01:27 AM (10 years, 3 days ago) |
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Oh I see. Thanks for the ongoing advice bodhisatta. I'm glad this thread will be full of information for other people to learn from in their search results too.
The petri dishes defect is that they have small particles in them but are apparently still sterile. (plastic debris or something)
The SAB I use is a tub. I hang the edge over the edge of a table just wide enough to fit my forearms in. I wear a dust mask to muffle my breathing and long rubber gloves wiped down with alcohol(lysol when using spirit burner). I mist the inside really heavily with ethanol then let it sit for a few mins before working in it. I close all doors and windows to reduce air currents and slow my breathing and move directly and efficiently (I do my best not to make abrupt movements that will blow dust around).
So far anything that involves agar work and flame sterilising scalpel/innoculating loop (I took nichrome wire loop,with an empty pen as handle) I work inside a clear plastic bag sprayed with lysol. I flame sterilise outside of the bag, then while still glowing red I bring it inside it and partially slide the agar open and cool the tip and begin cutting or swabbing etc).
I'm pretty convinced now that my pinsets have been crap due to the substrates not being properly consolidated now when I think of it. Which might of been because of making mistakes regarding the water content of the straw. I had a feeling that the straw looked too wet and the really wet parts struggled to colonised thickly so I aired my bags out and let some moisture evaporate. This caused premature pinning so I just fruited them. Just following the notion of "the mycelium knows what it's doing - just let it do its thing"
I aim to make a mono culture from a clone, then from there transfer the wedge to a grain jar, then do a G2G to make more spawn. This is where building a proper SAB will make or break my progress so far. I will be making monotubs again: I've got straw, cowpoo, verm, coco coir to work with. Have gypsum but I have the wrong lime so I want to buy the right one before I start again.
My spore prints were made a bit carelessly. but I do have a spore print from a reputable sponsor vendor which I could use to make a syringe or I can just try innoc some agar with my below average prints and go wedge to jar from there. Nothing to lose right?
Good luck blindingleaf!
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cronicr



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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: Watamiseh]
#19484475 - 01/28/14 01:29 AM (10 years, 3 days ago) |
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sounds like you got a solid plan laid out
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mushmagic
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: cronicr]
#19484478 - 01/28/14 01:31 AM (10 years, 3 days ago) |
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Even if your prints are "below average" as you say, you can still clean them up on the agar.
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blindingleaf
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Re: Salvaging ruined 35 gallon SGFC, how to tape it? + Hygrometer Q' [Re: mushmagic]
#19484895 - 01/28/14 06:01 AM (10 years, 3 days ago) |
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yea, i'd say skip the syringe, just use ur loop to swipe some spores on to ur agar.
and i know what u mean with straw water content. happened to me with oysters. i was thinking of trying a 30 min presoak next time, or just skipping it all together to see what happens, cause even when i let it dry out 24 hours, it seems too wet. I've never had a straw bag stop growing because of this, but i've had them take their time.
ur SAB tote set up sounds like my first one!! now i have one with arm holes burnt in, i cover those with press n seal and use a razor to make an X for my arms to go in, so there is much less gap/open space between my arm and the perimeter of the hole.
glad to see ur pretty organized in terms of making plans!
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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