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PsilliCoder
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Registered: 12/10/09
Posts: 764
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LC shelf life in refrigerator
#19388157 - 01/08/14 01:18 PM (10 years, 22 days ago) |
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I've got a liquid culture..honey or karo, I don't remember which I used..But it's been sitting in the refrigerator for a couple months now as I haven't had a chance to use it. How long will it last this way? Or is it already too late for it?
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tripdawg420
low life with no life



Registered: 02/02/09
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Re: LC shelf life in refrigerator [Re: PsilliCoder]
#19388173 - 01/08/14 01:22 PM (10 years, 22 days ago) |
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only one way to find out
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36fuckin5
Alchemycologist


Registered: 08/11/03
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Re: LC shelf life in refrigerator [Re: tripdawg420]
#19388213 - 01/08/14 01:32 PM (10 years, 22 days ago) |
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It should last a couple years really.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Paulsage
Shaman of shit storms



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Re: LC shelf life in refrigerator [Re: PsilliCoder]
#19388230 - 01/08/14 01:35 PM (10 years, 22 days ago) |
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Quote:
PsilliCoder said: I've got a liquid culture..honey or karo, I don't remember which I used..But it's been sitting in the refrigerator for a couple months now as I haven't had a chance to use it. How long will it last this way? Or is it already too late for it?
I've read everything from 6 months to yearrrrrrs... only way to know is to fill up a syringe and noc a jar up.
noc noc noc...
-------------------- "The brain is a reducing valve that restricts consciousness" - A Huxley "Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson… "Whatever you study you also change" - Heisenberg Uncertainty principle
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tripdawg420
low life with no life



Registered: 02/02/09
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Re: LC shelf life in refrigerator [Re: 36fuckin5]
#19388231 - 01/08/14 01:35 PM (10 years, 22 days ago) |
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: LC shelf life in refrigerator [Re: 36fuckin5]
#19388247 - 01/08/14 01:40 PM (10 years, 22 days ago) |
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Quote:
36fuckin5 said: It should last a couple years really.
 if you used the proper filter material then no problem
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PsilliCoder
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Registered: 12/10/09
Posts: 764
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Re: LC shelf life in refrigerator [Re: tripdawg420]
#19388293 - 01/08/14 01:49 PM (10 years, 22 days ago) |
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Quote:
tripdawg420 said:
only one way to find out 
I guess that's how i'm going to find out.
I didn't use a filter at all. Just a self healing injection port. I guess it probably does need some fresh air...
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: LC shelf life in refrigerator [Re: PsilliCoder]
#19388306 - 01/08/14 01:52 PM (10 years, 22 days ago) |
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no it needs just enough ge to stop a vacuum from happening
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PsilliCoder
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Registered: 12/10/09
Posts: 764
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Re: LC shelf life in refrigerator [Re: PsilliCoder]
#19388315 - 01/08/14 01:54 PM (10 years, 22 days ago) |
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Well i'll get some clean air in a syringe and make sure I stick that in it first to make sure it doesn't suck in a bunch of contams.
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36fuckin5
Alchemycologist


Registered: 08/11/03
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Re: LC shelf life in refrigerator [Re: cronicr]
#19388318 - 01/08/14 01:55 PM (10 years, 22 days ago) |
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It'll even still grow in a vacuum, just not as well.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: LC shelf life in refrigerator [Re: 36fuckin5]
#19388323 - 01/08/14 01:57 PM (10 years, 22 days ago) |
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Quote:
PsilliCoder said: Well i'll get some clean air in a syringe and make sure I stick that in it first to make sure it doesn't suck in a bunch of contams.

Quote:
36fuckin5 said: It'll even still grow in a vacuum, just not as well.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PsilliCoder
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Registered: 12/10/09
Posts: 764
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Re: LC shelf life in refrigerator [Re: PsilliCoder]
#19388349 - 01/08/14 02:05 PM (10 years, 22 days ago) |
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I don't really need it to grow. It's already consumed all the nutrients i'm sure.
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: LC shelf life in refrigerator [Re: PsilliCoder]
#19388353 - 01/08/14 02:06 PM (10 years, 22 days ago) |
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and it did so all with a vacuum
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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invitro


Registered: 05/03/13
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Re: LC shelf life in refrigerator [Re: 36fuckin5]
#19388455 - 01/08/14 02:34 PM (10 years, 22 days ago) |
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Myc will grow in a vaccum? That's news to me, I had 4 pint jars of honey lc that were in the fridge most of the time for about 4 months. I tested them all out and they were all alive. Those four jars had sfds. I also has a couple of syringes of myc from those jars that I sucked up when the growth was fresh and before refrigeration. I used them for inoculation and they were good but I didn't use the whole syringe so I also put them in the fridge with the 4 jars and waited 4-5 months. I tried both syringes and I could see the myc in there. I tried them on 6 1/4 pint jars, all six jars failed to show any growth. I figured they suffocated in the syringe but now I'm not sure. One syringe showed snow white hyphae, the other turned yellowish like metabolites in the water maybe.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: LC shelf life in refrigerator [Re: invitro]
#19388469 - 01/08/14 02:37 PM (10 years, 22 days ago) |
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well a syringe doesn't have a vacuum, did you have any air in it?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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invitro


Registered: 05/03/13
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Re: LC shelf life in refrigerator [Re: cronicr]
#19388481 - 01/08/14 02:41 PM (10 years, 22 days ago) |
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I had air, I had like 15ccs of liquid and 5ccs of air. No contams showed in the test jars, just sterile. So I re-did them with another lc and they took right off. I figured they used up the air in the 5ccs and then died for lack of oxygen.
Edited by invitro (01/08/14 02:43 PM)
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
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Re: LC shelf life in refrigerator [Re: invitro]
#19388553 - 01/08/14 02:55 PM (10 years, 22 days ago) |
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funny this came up...im about to noc up some rye with a honey lc started with agar. no ge hole just silicone. i was wondering...should i PC the syringe wrapped in foil with some air in it before puncturing the rtv?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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slh980
Average Idiot



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Re: LC shelf life in refrigerator [Re: blindingleaf]
#19388586 - 01/08/14 03:02 PM (10 years, 22 days ago) |
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This post made me think about LC, I remembered I have some. I got a couple jars of LC sitting on a shelf behind some newer ones that are apparently from 8+ months ago, gonna put some on agar tonight and see if that shit still works, thought I had lost my Orissa india but if this LC is alive I'm good 
It's in a quart jar, literally a mass of myc. or something.. hopefully myc!
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: LC shelf life in refrigerator [Re: blindingleaf]
#19388611 - 01/08/14 03:07 PM (10 years, 22 days ago) |
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Quote:
blindingleaf said: funny this came up...im about to noc up some rye with a honey lc started with agar. no ge hole just silicone. i was wondering...should i PC the syringe wrapped in foil with some air in it before puncturing the rtv?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Paulsage
Shaman of shit storms



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Re: LC shelf life in refrigerator [Re: slh980]
#19388644 - 01/08/14 03:13 PM (10 years, 22 days ago) |
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Quote:
It's in a quart jar, literally a mass of myc. or something.. hopefully myc!
time to bust out the mag stirrer and break that fucker up...
-------------------- "The brain is a reducing valve that restricts consciousness" - A Huxley "Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson… "Whatever you study you also change" - Heisenberg Uncertainty principle
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blindingleaf
blue collar underworld



Registered: 07/19/13
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Re: LC shelf life in refrigerator [Re: Paulsage]
#19388712 - 01/08/14 03:25 PM (10 years, 22 days ago) |
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i don't usually fuck with lc now that i have agar, but I've been bored, so i make tall 1/2 pints lc's for one time use (nocced up with a clean agar wedge), then toss. qt jars r too big to use for lc's for me. actually, taking an idea from cron's agar dishes, i think my next lc will be a mini one made from a spice jar, so its even smaller. the closer u can get the needle to the myc in the water without really messing with the jar, the better, IME and a mini spice container is PERFECT for that.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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1down5up
Social Ninja



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Re: LC shelf life in refrigerator [Re: blindingleaf]
#19388959 - 01/08/14 04:14 PM (10 years, 22 days ago) |
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These pp5's are great man...small, stackable, and you can see right through them....
 
The one on the right is a glass jar, but it's pretty difficult to make out what's going on in there.
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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Paulsage
Shaman of shit storms



Registered: 09/01/12
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Re: LC shelf life in refrigerator [Re: 1down5up]
#19389052 - 01/08/14 04:31 PM (10 years, 22 days ago) |
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Quote:
The one on the right is a glass jar, but it's pretty difficult to make out what's going on in there.
that is one thick ass LC...
impressive...
-------------------- "The brain is a reducing valve that restricts consciousness" - A Huxley "Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson… "Whatever you study you also change" - Heisenberg Uncertainty principle
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: LC shelf life in refrigerator [Re: Paulsage]
#19389166 - 01/08/14 04:44 PM (10 years, 22 days ago) |
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not a lc....brf/water
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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1down5up
Social Ninja



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Re: LC shelf life in refrigerator [Re: cronicr]
#19389258 - 01/08/14 04:57 PM (10 years, 22 days ago) |
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I used LC to knock them up...
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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Paulsage
Shaman of shit storms



Registered: 09/01/12
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Re: LC shelf life in refrigerator [Re: cronicr]
#19389463 - 01/08/14 05:23 PM (10 years, 22 days ago) |
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Quote:
cronicr said: not a lc....brf/water
sorry, long day at work.
after looking at the pics again, yah...
-------------------- "The brain is a reducing valve that restricts consciousness" - A Huxley "Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson… "Whatever you study you also change" - Heisenberg Uncertainty principle
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: LC shelf life in refrigerator [Re: Paulsage]
#19389476 - 01/08/14 05:25 PM (10 years, 22 days ago) |
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easy way to make a lc though
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blindingleaf
blue collar underworld



Registered: 07/19/13
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Re: LC shelf life in refrigerator [Re: cronicr]
#19389775 - 01/08/14 06:09 PM (10 years, 22 days ago) |
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so just used the lc i was talking about above, first time making one with no GE hole. PC'd syringe wrapped in foil 20 min with air inside (20ml) planning on using 10ml lc 2ml per jar (5 jars if u do ur math right). DAMN that shit had a vacuum like no other, even with the air in needle. i thought it would suck the air out of the needle as soon as it penetrated the RTV, but it didn't. i tried sucking up the lc into the syringe without expelling any air (it was sterile after all) and nothing would suck up, but the plunger WAS able to be pulled back. it was not until i expelled the air in syringe into the air of the lc jar that i would suck up any liquid, and even then, i could only suck up 7 ml of solution. weird. I'm no physics major though (though i can fly like a pig!). maybe there was a breach in the lid or rtv. also put a drop onto some agar, why not? only had one PP5 left to use, and it was between testing the lc (despite already knocking up jars) or taking clone flesh from a non sporulating fruit. maybe i made the wrong choice
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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invitro


Registered: 05/03/13
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Re: LC shelf life in refrigerator [Re: blindingleaf]
#19389872 - 01/08/14 06:27 PM (10 years, 22 days ago) |
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The way I understand it, if you have a mason jar with a rubber seal, when the air heats up it pushes the lid up a little as the air expands, letting air out. Then as it cools it pulls the lid down and the air continues to shrink as it cools, making a partial vacuum. So you have to add a bit of air, I guess more than 20ccs in your case.
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1down5up
Social Ninja



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Re: LC shelf life in refrigerator [Re: invitro]
#19393832 - 01/09/14 02:00 PM (10 years, 21 days ago) |
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You could avoid all that if you drill a very small hole in the lid (besides the SHIP hole) and cover it with some tyvek/SFD/or E felt. You could cut the tyvek same size as the metal insert of the lid, remove it to shake it up, so it doesn't get wet, and replace it when you're done so it can sit on a shelf protected.....
.02
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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36fuckin5
Alchemycologist


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Re: LC shelf life in refrigerator [Re: 1down5up]
#19394909 - 01/09/14 05:49 PM (10 years, 21 days ago) |
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You never remove your filter, and if you're gonna use a filter like that, you have to have a stir plate or else you'll just get a glob of myc that won't go into a syringe.
Use a Whatman filter, or use 2 injection ports and an airport syringe.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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1down5up
Social Ninja



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Re: LC shelf life in refrigerator [Re: 36fuckin5]
#19395027 - 01/09/14 06:05 PM (10 years, 21 days ago) |
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You can cover the hole in the lid and shake the LC jar to break up the mycelium. The only time you have to remove the filter is when you do this, otherwise it will get wet, and that's a no-no. So you don't really need a stir plate, or anything fancy. You can also safely remove the filter to facilitate this, and successfully. I've done it, and i'm a noob, so if i can, he/she can.
I placed a LC jar in SAB with nothing more than a tin foil lid on it, covered the hole with tape, shook the shit out of it, and used a sterile syringe to draw up the culture. I then used that culture to knock up 8 BRF agar sub jars, and all 8 are growing....they're still babies, but they're growing. No SHIP, no filter, no nothing on the LC jar, and it worked. So rather than getting into injecting air in a jar to stabilize pressure, it would make more sense to use a very tiny hole to do that for you, and cover it with some sort of filter for when it sits there doing it's thing.
Go to page 7 on this thread and watch me do it.
http://www.shroomery.org/forums/showflat.php/Number/19095135/fpart/7/vc/1#19095135
I took even less precautions than mentioned above, though i will def use a SFD with a SHIP next time i do LC
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
Edited by 1down5up (01/11/14 02:39 PM)
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36fuckin5
Alchemycologist


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Re: LC shelf life in refrigerator [Re: 1down5up]
#19403715 - 01/11/14 12:50 PM (10 years, 19 days ago) |
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Again, you NEVER remove your filter. Just because you got lucky on 8 bags doesn't mean it's a reliable method. This kind of thinking will ruin your future grows.
Do 800 and then come back and tell me how it goes.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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1down5up
Social Ninja



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Re: LC shelf life in refrigerator [Re: 36fuckin5]
#19404170 - 01/11/14 02:37 PM (10 years, 19 days ago) |
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THey weren't used on bags man..i used the LC to knock up small pp5 jars. IF you say not to remove it, I'll listen to you. You seem to know what you're talkin' about, based on some other posts i've read of yours. I'm glad I got lucky on those 8...cheers
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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