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InvisiblePsilliCoder
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Registered: 12/10/09
Posts: 764
LC shelf life in refrigerator
    #19388157 - 01/08/14 01:18 PM (10 years, 22 days ago)

I've got a liquid culture..honey or karo, I don't remember which I used..But it's been sitting in the refrigerator for a couple months now as I haven't had a chance to use it. How long will it last this way? Or is it already too late for it?


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Offlinetripdawg420
low life with no life
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Registered: 02/02/09
Posts: 7,071
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Re: LC shelf life in refrigerator [Re: PsilliCoder]
    #19388173 - 01/08/14 01:22 PM (10 years, 22 days ago)

:shrug: only one way to find out :thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: LC shelf life in refrigerator [Re: tripdawg420]
    #19388213 - 01/08/14 01:32 PM (10 years, 22 days ago)

It should last a couple years really.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflinePaulsage
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Registered: 09/01/12
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Re: LC shelf life in refrigerator [Re: PsilliCoder]
    #19388230 - 01/08/14 01:35 PM (10 years, 22 days ago)

Quote:

PsilliCoder said:
I've got a liquid culture..honey or karo, I don't remember which I used..But it's been sitting in the refrigerator for a couple months now as I haven't had a chance to use it. How long will it last this way? Or is it already too late for it?




I've read everything from 6 months to yearrrrrrs...  only way to know is to fill up a syringe and noc a jar up.

noc noc noc...  :rockon:


--------------------
"The brain is a reducing valve that restricts consciousness" - A Huxley

"Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson…

"Whatever you study you also change" - Heisenberg Uncertainty principle


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Offlinetripdawg420
low life with no life
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Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 6 hours, 13 minutes
Re: LC shelf life in refrigerator [Re: 36fuckin5]
    #19388231 - 01/08/14 01:35 PM (10 years, 22 days ago)

:thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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OfflinecronicrFacebook
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Posts: 61,436
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Re: LC shelf life in refrigerator [Re: 36fuckin5]
    #19388247 - 01/08/14 01:40 PM (10 years, 22 days ago)

Quote:

36fuckin5 said:
It should last a couple years really.



:whathesaid:
if you used the proper filter material then no problem


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisiblePsilliCoder
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Registered: 12/10/09
Posts: 764
Re: LC shelf life in refrigerator [Re: tripdawg420]
    #19388293 - 01/08/14 01:49 PM (10 years, 22 days ago)

Quote:

tripdawg420 said:
:shrug: only one way to find out :thumbup:




I guess that's how i'm going to find out.

I didn't use a filter at all. Just a self healing injection port. I guess it probably does need some fresh air...


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OfflinecronicrFacebook
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Re: LC shelf life in refrigerator [Re: PsilliCoder]
    #19388306 - 01/08/14 01:52 PM (10 years, 22 days ago)

no it needs just enough ge to stop a vacuum from happening


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisiblePsilliCoder
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Registered: 12/10/09
Posts: 764
Re: LC shelf life in refrigerator [Re: PsilliCoder]
    #19388315 - 01/08/14 01:54 PM (10 years, 22 days ago)

Well i'll get some clean air in a syringe and make sure I stick that in it first to make sure it doesn't suck in a bunch of contams.


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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,079
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Re: LC shelf life in refrigerator [Re: cronicr]
    #19388318 - 01/08/14 01:55 PM (10 years, 22 days ago)

It'll even still grow in a vacuum, just not as well.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
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Re: LC shelf life in refrigerator [Re: 36fuckin5]
    #19388323 - 01/08/14 01:57 PM (10 years, 22 days ago)

Quote:

PsilliCoder said:
Well i'll get some clean air in a syringe and make sure I stick that in it first to make sure it doesn't suck in a bunch of contams.



:bigyesnod:
Quote:

36fuckin5 said:
It'll even still grow in a vacuum, just not as well.



:thumbup:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisiblePsilliCoder
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Registered: 12/10/09
Posts: 764
Re: LC shelf life in refrigerator [Re: PsilliCoder]
    #19388349 - 01/08/14 02:05 PM (10 years, 22 days ago)

I don't really need it to grow. It's already consumed all the nutrients i'm sure.


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OfflinecronicrFacebook
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Re: LC shelf life in refrigerator [Re: PsilliCoder]
    #19388353 - 01/08/14 02:06 PM (10 years, 22 days ago)

and it did so all with a vacuum:rockon:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: LC shelf life in refrigerator [Re: 36fuckin5]
    #19388455 - 01/08/14 02:34 PM (10 years, 22 days ago)

Myc will grow in a vaccum? That's news to me, I had 4 pint jars of honey lc that were in the fridge most of the time for about 4 months.  I tested them all out and they were all alive.  Those four jars had sfds.  I also has a couple of syringes of myc from those jars that I sucked up when the growth was fresh and before refrigeration.  I used them for inoculation and they were good but I didn't use the whole syringe so I also put them in the fridge with the 4 jars and waited 4-5 months.  I tried both syringes and I could see the myc in there.  I tried them on 6 1/4 pint jars, all six jars failed to show any growth.  I figured they suffocated in the syringe but now I'm not sure.  One syringe showed snow white hyphae, the other turned yellowish like metabolites in the water maybe.


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OfflinecronicrFacebook
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Re: LC shelf life in refrigerator [Re: invitro]
    #19388469 - 01/08/14 02:37 PM (10 years, 22 days ago)

well a syringe doesn't have a vacuum, did you have any air in it?


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlineinvitro


Registered: 05/03/13
Posts: 2,529
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Re: LC shelf life in refrigerator [Re: cronicr]
    #19388481 - 01/08/14 02:41 PM (10 years, 22 days ago)

I had air, I had like 15ccs of liquid and 5ccs of air.  No contams showed in the test jars, just sterile.  So I re-did them with another lc and they took right off.  I figured they used up the air in the 5ccs and then died for lack of oxygen.


Edited by invitro (01/08/14 02:43 PM)


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Invisibleblindingleaf
blue collar underworld
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Registered: 07/19/13
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Re: LC shelf life in refrigerator [Re: invitro]
    #19388553 - 01/08/14 02:55 PM (10 years, 22 days ago)

funny this came up...im about to noc up some rye with a honey lc started with agar.  no ge hole just silicone.  i was wondering...should i PC the syringe wrapped in foil with some air in it before puncturing the rtv?


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MICROBIAL HUSBANDRY!!!!

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Offlineslh980
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Re: LC shelf life in refrigerator [Re: blindingleaf]
    #19388586 - 01/08/14 03:02 PM (10 years, 22 days ago)

This post made me think about LC, I remembered I have some. I got a couple jars of LC sitting on a shelf behind some newer ones that are apparently from 8+ months ago, gonna put some on agar tonight and see if that shit still works, thought I had lost my Orissa india but if this LC is alive I'm good :thumbup:

It's in a quart jar, literally a mass of myc. or something.. hopefully myc!


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OfflinecronicrFacebook
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Re: LC shelf life in refrigerator [Re: blindingleaf]
    #19388611 - 01/08/14 03:07 PM (10 years, 22 days ago)

Quote:

blindingleaf said:
funny this came up...im about to noc up some rye with a honey lc started with agar.  no ge hole just silicone.  i was wondering...should i PC the syringe wrapped in foil with some air in it before puncturing the rtv?



:bigyesnod:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinePaulsage
Shaman of shit storms
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Registered: 09/01/12
Posts: 109
Last seen: 6 years, 5 months
Re: LC shelf life in refrigerator [Re: slh980]
    #19388644 - 01/08/14 03:13 PM (10 years, 22 days ago)

Quote:

It's in a quart jar, literally a mass of myc. or something.. hopefully myc!




time to bust out the mag stirrer and break that fucker up...

:thumbup:


--------------------
"The brain is a reducing valve that restricts consciousness" - A Huxley

"Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson…

"Whatever you study you also change" - Heisenberg Uncertainty principle


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Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures


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