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Offlinehotshot98456
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Can i use the spores that popped in my fc?
    #19386503 - 01/08/14 04:10 AM (10 years, 1 month ago)

There is a near perfect spore print on the aluminum foil that was underneath the cake from a mushroom that grew from underneath it. Then theres also alot of spores on the perlite right next to it, is it possible to use these for a spore syringe if i were to use them fresh? Any opinions and or knowledge would help


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Offlinedceodhz
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Re: Can i use the spores that popped in my fc? [Re: hotshot98456]
    #19386506 - 01/08/14 04:14 AM (10 years, 1 month ago)

Its NOT a clean print. You could try isolating on agar or use another cap to create a sterile print.


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Invisiblemushmagic
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Re: Can i use the spores that popped in my fc? [Re: hotshot98456]
    #19386507 - 01/08/14 04:15 AM (10 years, 1 month ago)

It would be better to use a properly prepared print for syringe (not to say you couldn't get away with it, it just wouldn't be as ideal). Or you could always clean it up with a few transfers on agar.


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Invisiblemushmagic
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Re: Can i use the spores that popped in my fc? [Re: dceodhz]
    #19386510 - 01/08/14 04:16 AM (10 years, 1 month ago)

Quote:

dceodhz said:
Its NOT a clean print. You could try isolating on agar or use another cap to create a sterile print.




No print would be completely clean/sterile unless it was grown and printed under completely sterile condition.


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Offlinedceodhz
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Re: Can i use the spores that popped in my fc? [Re: mushmagic]
    #19386540 - 01/08/14 04:54 AM (10 years, 1 month ago)

"Clean" is relative on these forums


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Invisiblemushmagic
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Re: Can i use the spores that popped in my fc? [Re: dceodhz]
    #19387862 - 01/08/14 12:06 PM (10 years, 1 month ago)

Quote:

dceodhz said:
"Clean" is relative on these forums




Yes but you also said to use another cap to create a "sterile print" though which isn't possible because it wasn't grown under sterile conditions. You are correct in that it would produce a much "cleaner" print than using the one from the foil in the FC.


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InvisiblebodhisattaMDiscordReddit
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Re: Can i use the spores that popped in my fc? [Re: mushmagic]
    #19387887 - 01/08/14 12:11 PM (10 years, 1 month ago)

You can't have a sterile print, you need shroom spores on it, hence not sterile.

You want a clean print, then use new foil not stuff that's been siting around in open air for weeks. Do it in a SAB, or use in-vitro fruits.


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Invisiblemushmagic
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Re: Can i use the spores that popped in my fc? [Re: bodhisatta]
    #19387899 - 01/08/14 12:14 PM (10 years, 1 month ago)

:whathesaid:


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Offlinetripdawg420
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Re: Can i use the spores that popped in my fc? [Re: mushmagic]
    #19388000 - 01/08/14 12:40 PM (10 years, 1 month ago)

when i take prints i put my fc in the still air room right before the caps open and tear and let them open in still air wont hurt them :thumbup:


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InvisibleWillSolvem
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Re: Can i use the spores that popped in my fc? [Re: tripdawg420]
    #19388068 - 01/08/14 12:57 PM (10 years, 1 month ago)

I've done something similar. I had a cyan grow in a tub, they can be tricky to print sometimes. While dumping the substrate I noticed a partial print. I really didn't believe I could pull it off, thought it would be a lesson in trichology. I swabbed with a sterile swab and transferred to agar. Cyan mycelium will colonize fast and this worked in my benefit. After a few transfers I had a clean culture. I've also have had plenty of prints from shroomers that I failed to clean. If you pour your own plates what do you have to loose besides a couple hours of your time and a pinch of agar?

I say go for it, you will learn even if you fail.


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