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Doc Seta
Sinister Das Trip Sober



Registered: 12/18/13
Posts: 550
Loc: Tunafish, Ocean
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Got Gill in my Syringe? Does this matter or Pass it Up?
#19387367 - 01/08/14 10:46 AM (10 years, 1 month ago) |
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Yea so I made up a spore syringe of my favorite Shittakes edibles and wanted some feedback before I mess anything up. Some gill matter got mixed with the spores. I'm like shaking it , dancing , somersolts fucking karate kicks and it just spins back into the dark fat black blob of nasty twist. Anyway my question is can this cause me problems contam wise cause I don't wanna waste quarts of grain jars after all the work of preparation ya know. I still have some left chilling I bought, idk any feedback is appreciated and if needed I can take pix later no white fluffies germination yuck, its that damn strain where the gill won't disconnect if ya know what pink buffalo I mean. Lol
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: Doc Seta]
#19387383 - 01/08/14 10:48 AM (10 years, 1 month ago) |
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Put it to agar. Spore syringes are never 100% clean. Agar cultures can be 100% clean. Worst case scenario it could be contam without the gills anyway, use agar. Or the gills could be bad, use agar, or it's clean, or you inadvertently get a liquid culture.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 23 hours, 16 minutes
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: bodhisatta]
#19387389 - 01/08/14 10:50 AM (10 years, 1 month ago) |
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Doc Seta
Sinister Das Trip Sober



Registered: 12/18/13
Posts: 550
Loc: Tunafish, Ocean
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: tripdawg420]
#19387459 - 01/08/14 11:05 AM (10 years, 1 month ago) |
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Yea true, there is spores obviously but for that clump of gill floating like a blood clot makes me wonder wtf .. (this could go bad man don't do it). I'm scared to use it but sucks cause its such a good strain imo & ime, thanks for the feedback bro
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: Doc Seta]
#19387477 - 01/08/14 11:08 AM (10 years, 1 month ago) |
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shoot up a test cake first, you could make a small one with a glass from your kitchen probably, or even a half ass grain flour paste petri dish or something,
Edited by Trusted cuItivator (01/08/14 11:08 AM)
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Doc Seta
Sinister Das Trip Sober



Registered: 12/18/13
Posts: 550
Loc: Tunafish, Ocean
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: bodhisatta]
#19387555 - 01/08/14 11:25 AM (10 years, 1 month ago) |
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Quote:
bodhisatta said: shoot up a test cake first, you could make a small one with a glass from your kitchen probably, or even a half ass grain flour paste petri dish or something,
Yea I always throw a few 1/4 pints on top for experimention aspects which have all gone pretty well so far. I guess I got some rice to grind right now for the buffallo just don't wanna mess up my spawn quarts which you & I know take a lil extra TLC when preparing. Minds made up it shall go to the experimental lounge . I'm overstocked on that KSSS & another which happens to be the good rynges waiting on standby. Thanks for helping decide this bro
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CliftonGK1
Sasquatch



Registered: 03/27/13
Posts: 327
Loc: A place
Last seen: 24 days, 20 hours
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: Doc Seta]
#19387833 - 01/08/14 11:59 AM (10 years, 1 month ago) |
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Quote:
Doc Seta said: I'm scared to use it but sucks cause its such a good strain imo & ime, thanks for the feedback bro
Experienced cultivators correct me if I'm wrong, but if you want to propagate a specific feature for a strain, isn't sectioning to agar the preferred method because a spore syringe will result in variable genetics?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: CliftonGK1]
#19387848 - 01/08/14 12:03 PM (10 years, 1 month ago) |
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yep
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Doc Seta
Sinister Das Trip Sober



Registered: 12/18/13
Posts: 550
Loc: Tunafish, Ocean
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: CliftonGK1]
#19387864 - 01/08/14 12:06 PM (10 years, 1 month ago) |
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Quote:
CliftonGK1 said:
Quote:
Doc Seta said: I'm scared to use it but sucks cause its such a good strain imo & ime, thanks for the feedback bro
Experienced cultivators correct me if I'm wrong, but if you want to propagate a specific feature for a strain, isn't sectioning to agar the preferred method because a spore syringe will result in variable genetics?
Talking strain here bro not some isolate I got cooked up on a plate too lazy for that now. Pink Buff is a good strain ime & imo , I like the way u tried to pull that snobby tone off tho, props.
Edited by Doc Seta (01/08/14 12:09 PM)
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: Doc Seta]
#19387875 - 01/08/14 12:09 PM (10 years, 1 month ago) |
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a strain is an isolate, all you have with spores is the ability to produce an specific organism like yellow oysters and blue oysters, not any specific quality of them though(without isolation) just a mixed bag.
Edited by Trusted cuItivator (01/08/14 12:10 PM)
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Doc Seta
Sinister Das Trip Sober



Registered: 12/18/13
Posts: 550
Loc: Tunafish, Ocean
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: bodhisatta]
#19387911 - 01/08/14 12:16 PM (10 years, 1 month ago) |
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That's the deal , took a print off Pink Buff gill got mixed in didn't go the extra yards of agar with it an all that wonderful stuff. Back to business now , and I never asked in the master cultivation section. Thanks for the replys
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: Doc Seta]
#19387935 - 01/08/14 12:21 PM (10 years, 1 month ago) |
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We just like to tie up loose ends for any lurkers and noobs that might come read this or search and find it in the future.
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CliftonGK1
Sasquatch



Registered: 03/27/13
Posts: 327
Loc: A place
Last seen: 24 days, 20 hours
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: Doc Seta]
#19389323 - 01/08/14 05:05 PM (10 years, 1 month ago) |
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Quote:
Doc Seta said: Talking strain here bro not some isolate I got cooked up on a plate too lazy for that now. Pink Buff is a good strain ime & imo , I like the way u tried to pull that snobby tone off tho, props.
Snobby tone? Seriously? Have you seen my other posts here? I readily admit that my background isn't mycology (it's clin micro and virology), and more of what I've posted recently has been the attempted cleanup of a culture I didn't recognize as contaminated. Whatever. Lighten up, man. All I did was ask a question.
As for gill material in a syringe, at most it may pose a higher contamination risk as it may have been a settling point for contaminants during fruiting. Considering that you can clone out from cuttings off caps, stems, or the case of the cleanup I'm doing now, pins from a prior plate, I don't see that it would be an issue.
At the very least, use it as an experiment. What's the worst that happens? A jar or two goes tits up and you start over again. At best, you end up with at least one jar that works.
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Doc Seta
Sinister Das Trip Sober



Registered: 12/18/13
Posts: 550
Loc: Tunafish, Ocean
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Re: Got Gill in my Syringe? Does this matter or Pass it Up? [Re: CliftonGK1]
#19389380 - 01/08/14 05:13 PM (10 years, 1 month ago) |
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No offense there u just came out like u were trolling its hard to tell now on here ya know. No hard feelings bro an yea Im gonna use it for my extra experiment jars on the side. Didn't wanna mess up freshly made sterile grain jars that are a pain to make. Anyway Ill see ya around bro good to know u werent trolling lol later mane
Edited by Doc Seta (01/08/14 05:15 PM)
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