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hotshot98456
Stranger


Registered: 12/30/13
Posts: 42
Last seen: 1 year, 7 months
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Can i use the spores that popped in my fc?
#19386503 - 01/08/14 04:10 AM (10 years, 1 month ago) |
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There is a near perfect spore print on the aluminum foil that was underneath the cake from a mushroom that grew from underneath it. Then theres also alot of spores on the perlite right next to it, is it possible to use these for a spore syringe if i were to use them fresh? Any opinions and or knowledge would help
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dceodhz
Up And Coming

Registered: 10/19/13
Posts: 78
Loc: That's NO Moon
Last seen: 6 years, 4 months
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Re: Can i use the spores that popped in my fc? [Re: hotshot98456]
#19386506 - 01/08/14 04:14 AM (10 years, 1 month ago) |
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Its NOT a clean print. You could try isolating on agar or use another cap to create a sterile print.
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Can i use the spores that popped in my fc? [Re: hotshot98456]
#19386507 - 01/08/14 04:15 AM (10 years, 1 month ago) |
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It would be better to use a properly prepared print for syringe (not to say you couldn't get away with it, it just wouldn't be as ideal). Or you could always clean it up with a few transfers on agar.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Can i use the spores that popped in my fc? [Re: dceodhz]
#19386510 - 01/08/14 04:16 AM (10 years, 1 month ago) |
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Quote:
dceodhz said: Its NOT a clean print. You could try isolating on agar or use another cap to create a sterile print.
No print would be completely clean/sterile unless it was grown and printed under completely sterile condition.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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dceodhz
Up And Coming

Registered: 10/19/13
Posts: 78
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Last seen: 6 years, 4 months
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Re: Can i use the spores that popped in my fc? [Re: mushmagic]
#19386540 - 01/08/14 04:54 AM (10 years, 1 month ago) |
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"Clean" is relative on these forums
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Can i use the spores that popped in my fc? [Re: dceodhz]
#19387862 - 01/08/14 12:06 PM (10 years, 1 month ago) |
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Quote:
dceodhz said: "Clean" is relative on these forums
Yes but you also said to use another cap to create a "sterile print" though which isn't possible because it wasn't grown under sterile conditions. You are correct in that it would produce a much "cleaner" print than using the one from the foil in the FC.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Can i use the spores that popped in my fc? [Re: mushmagic]
#19387887 - 01/08/14 12:11 PM (10 years, 1 month ago) |
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You can't have a sterile print, you need shroom spores on it, hence not sterile.
You want a clean print, then use new foil not stuff that's been siting around in open air for weeks. Do it in a SAB, or use in-vitro fruits.
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Can i use the spores that popped in my fc? [Re: bodhisatta]
#19387899 - 01/08/14 12:14 PM (10 years, 1 month ago) |
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
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Re: Can i use the spores that popped in my fc? [Re: mushmagic]
#19388000 - 01/08/14 12:40 PM (10 years, 1 month ago) |
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when i take prints i put my fc in the still air room right before the caps open and tear and let them open in still air wont hurt them
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WillSolvem
Odd-Hand




Registered: 04/24/12
Posts: 1,519
Loc: Chapter 26
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Re: Can i use the spores that popped in my fc? [Re: tripdawg420]
#19388068 - 01/08/14 12:57 PM (10 years, 1 month ago) |
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I've done something similar. I had a cyan grow in a tub, they can be tricky to print sometimes. While dumping the substrate I noticed a partial print. I really didn't believe I could pull it off, thought it would be a lesson in trichology. I swabbed with a sterile swab and transferred to agar. Cyan mycelium will colonize fast and this worked in my benefit. After a few transfers I had a clean culture. I've also have had plenty of prints from shroomers that I failed to clean. If you pour your own plates what do you have to loose besides a couple hours of your time and a pinch of agar?
I say go for it, you will learn even if you fail.
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AMU Q&A Thread because questions deserve answers.
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