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OfflinePaulsage
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Numbering agar plates
    #19384145 - 01/07/14 06:10 PM (10 years, 23 days ago)

I'm about to get started doing agar, and i was wondering how some of you guys number and track your plates and generations.  is there a standard, or does everyone have their own method?

love to know...


--------------------
"The brain is a reducing valve that restricts consciousness" - A Huxley

"Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson…

"Whatever you study you also change" - Heisenberg Uncertainty principle


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InvisiblebodhisattaMDiscordReddit
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Re: Numbering agar plates [Re: Paulsage]
    #19384171 - 01/07/14 06:16 PM (10 years, 23 days ago)

I think most people have their own method. A good few things to indicate are the date you made the dish, the date you inoculated it(if its different), the variety, how many transfers it is(MS 2nd.. or from clone tissue etc...), the kind of agar it is if you use more than one and can't tell them apart by color of the media, etc.. and anything else that's important to you


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OfflineYuri.Pono
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Re: Numbering agar plates [Re: bodhisatta]
    #19384235 - 01/07/14 06:28 PM (10 years, 23 days ago)

always label and log in notebook to keep track of truecrypt usb  folder

type: p.cubenis amazon
date: 01/01/0001
gen: 1st

for transfers try

plate one: aa1
transfer: ab1
2nd transfer:ac1

type: p.cubenis amazon
date: 01/01/0001
gen: 1st

plate one: ba1
transfer: bb1
2nd transfer:bc1

hope that makes since


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Edited by Yuri.Pono (01/07/14 06:36 PM)


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OfflinecronicrFacebook
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Re: Numbering agar plates [Re: Yuri.Pono]
    #19384260 - 01/07/14 06:33 PM (10 years, 23 days ago)

i don't label mine by gens, for proper gens they need to be fruioted all your doing is expanding the original gen


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OfflinePaulsage
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Re: Numbering agar plates [Re: Yuri.Pono]
    #19384532 - 01/07/14 07:22 PM (10 years, 23 days ago)

Quote:

Yuri.Pono said:
always label and log in notebook to keep track of truecrypt usb  folder





i never keep a notebook, keep everything in my head based on where i put something.  hard to explain but it works for me...  plus nothing for someone else to pick up and start to read...

your method does make sense...


--------------------
"The brain is a reducing valve that restricts consciousness" - A Huxley

"Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson…

"Whatever you study you also change" - Heisenberg Uncertainty principle


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OfflinecronicrFacebook
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Re: Numbering agar plates [Re: Paulsage]
    #19384541 - 01/07/14 07:23 PM (10 years, 23 days ago)

I  just label mine as species/variety,date and transfer number


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinetwistedty
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Re: Numbering agar plates [Re: Paulsage]
    #19384551 - 01/07/14 07:25 PM (10 years, 23 days ago)

what you need to really keep track of is what fruited the best and make a culture slant for long term cold storage.


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OfflinePaulsage
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Re: Numbering agar plates [Re: twistedty]
    #19384682 - 01/07/14 07:46 PM (10 years, 23 days ago)

Quote:

twistedty said:
what you need to really keep track of is what fruited the best and make a culture slant for long term cold storage.




totally, but i'm not there yet.  need to get something on agar first...  but yah, if i'm able to get a really nice isolation then keeping a slant is definitely something i'm going to add to my bag of tricks...


--------------------
"The brain is a reducing valve that restricts consciousness" - A Huxley

"Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson…

"Whatever you study you also change" - Heisenberg Uncertainty principle


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OfflinePaulsage
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Re: Numbering agar plates [Re: cronicr]
    #19384694 - 01/07/14 07:49 PM (10 years, 23 days ago)

Quote:

cronicr said:
I  just label mine as species/variety,date and transfer number




i think that's what i'm kind of going to do...  but since i'll be taking the best bits off of my first plate that might mean more than 1 transfer to a second gen.  so i'll label them a bit differently...  i can see it in my head...

thanks guys... 

:billymaythumbup:


--------------------
"The brain is a reducing valve that restricts consciousness" - A Huxley

"Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson…

"Whatever you study you also change" - Heisenberg Uncertainty principle


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Offlinetwistedty
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Re: Numbering agar plates [Re: Paulsage]
    #19384706 - 01/07/14 07:51 PM (10 years, 23 days ago)

Quote:

Paulsage said:
Quote:

cronicr said:
I  just label mine as species/variety,date and transfer number




i think that's what i'm kind of going to do...  but since i'll be taking the best bits off of my first plate that might mean more than 1 transfer to a second gen.  so i'll label them a bit differently...  i can see it in my head...

thanks guys... 

:billymaythumbup:




just make sure you keep track of what dish inoculated what, so if you get yourself a NICE PINSET you can always go back to said dish


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Offlinetwistedty
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Re: Numbering agar plates [Re: twistedty]
    #19384710 - 01/07/14 07:52 PM (10 years, 23 days ago)

agar is :winning:

i rated you +5 for your jump to agar


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InvisiblebodhisattaMDiscordReddit
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Re: Numbering agar plates [Re: twistedty]
    #19384731 - 01/07/14 07:55 PM (10 years, 23 days ago)

Quote:

twistedty said:
agar is :winning:

i rated you +5 for your jump to agar



:thumbup:


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OfflinePaulsage
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Re: Numbering agar plates [Re: twistedty]
    #19384761 - 01/07/14 08:01 PM (10 years, 23 days ago)

Quote:

twistedty said:
agar is :winning:

i rated you +5 for your jump to agar




disco - thx man...


--------------------
"The brain is a reducing valve that restricts consciousness" - A Huxley

"Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson…

"Whatever you study you also change" - Heisenberg Uncertainty principle


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