|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Cogsy
Psychedelic cultivator


Registered: 11/08/13
Posts: 265
Last seen: 7 years, 2 months
|
|
I sort of agree with you... I'm not quite that zealous though... Tub in tub incubation works. As long as you are using it properly and not fryinging your substrate
-------------------- great write-up for new-guys ___________________________________________________________
  ____________________________________________________________ Cogsy
|
twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
|
|
woooahhhhh settle down whippy, just kidding i agree with you 100% this being the strong point
Quote:
Whippy said: Even in the best of conditions, it may save you a few days of colonization. It don't matter how you define the costs, it the cons outweigh the benefits.
|
twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
|
Re: Cold House. Need HEAT PAD ASAP! [Re: twistedty]
#19383959 - 01/07/14 05:37 PM (10 years, 23 days ago) |
|
|
i would need quite a few TITs to keep my grows warm
|
Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
|
Re: Cold House. Need HEAT PAD ASAP! [Re: twistedty]
#19383960 - 01/07/14 05:37 PM (10 years, 23 days ago) |
|
|
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
Edited by Yuri.Pono (01/07/14 05:39 PM)
|
HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
|
Re: Cold House. Need HEAT PAD ASAP! [Re: Cogsy]
#19383963 - 01/07/14 05:38 PM (10 years, 23 days ago) |
|
|
Quote:
Cogsy said: I sort of agree with you... I'm not quite that zealous though...
Not zealous, just trying to impress upon others a bit of logic. Warmth does not mystically create mold, or most places near the equator would be uninhabitable.
|
Third Eye Vision
Part of a rebel alliance


Registered: 11/13/13
Posts: 66
Last seen: 3 years, 9 months
|
|
my house is super cold also. no central heat/cold 
tried on top of fridge, but wasnt getting the job done. just got a small space heater for my room. now my room is a cozy 70 degrees and my cakes colonize super fast!
-------------------- "If a man does not keep pace with his companions, perhaps it is because he hears a different drummer." Henry David Thoreau
|
HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
|
|
Quote:
Yuri.Pono said: http://www.homedepot.com/p/Pelonis-1-500-Watt-Electric-Oil-Filled-Radiant-Portable-Heater-HO-0218H/202295910
works great for my room. no more TiTs required except from the wifey
Quote:
Third Eye Vision said: my house is super cold also. no central heat/cold 
tried on top of fridge, but wasnt getting the job done. just got a small space heater for my room. now my room is a cozy 70 degrees and my cakes colonize super fast!
The heater listed above coupled with a small box fan is my central heat for my office area. The rest of the house is nice and cozy cause they have 20th century amenities (i.e. heat)....I still have the warmest room in the house.
|
Cogsy
Psychedelic cultivator


Registered: 11/08/13
Posts: 265
Last seen: 7 years, 2 months
|
|
Quote:
Whippy said: Warmth does not mystically create mold, or most places near the equator would be uninhabitable.
Word. But used incorrectly it is a negative, and unnecessary incubation should be avoided, Just my thoughts
-------------------- great write-up for new-guys ___________________________________________________________
  ____________________________________________________________ Cogsy
|
HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
|
Re: Cold House. Need HEAT PAD ASAP! [Re: Cogsy]
#19384010 - 01/07/14 05:47 PM (10 years, 23 days ago) |
|
|
Quote:
Cogsy said: But used incorrectly it is a negative, and unnecessary incubation should be avoided, Just my thoughts
I agree totally. Keeping it simple is the best way to do it. 5 days without signs of growth is totally normal. I have waited up to 3 weeks for grain bags to show anything from MSS. The best way to see fast growth is to switch to agar (I will not recommend liquid culture, though I have had success).
|
bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
|
The heat doesn't cause contams necessarily. Sterilization isn't ever complete in PF jars steamed for 90M or grain jars PCed for 120M at 15PSI you still have some endospore that can germinate. I don't think any one can for sure say BRF has 0 endospores just less and they're smaller and easier to be penetrated by steam. Either way if you leave them out long enough and they don't dry out something will probably eventually grow in some percentage of your jars long after mycelium would have normally got to 100% colonization. We sterilize to operate within a reasonable window. An incubator can shift that window. That's what I know at least, where am I wrong?
|
Cogsy
Psychedelic cultivator


Registered: 11/08/13
Posts: 265
Last seen: 7 years, 2 months
|
Re: Cold House. Need HEAT PAD ASAP! [Re: bodhisatta]
#19384149 - 01/07/14 06:11 PM (10 years, 23 days ago) |
|
|
Nowhere as far as I can see regarding steam sterilisation, I'm not sure regarding pressure cooking however.. And don't most contaminants outgrow mycelium in all temperatures really? If a contaminant survived the sterilisation process wouldn't it be better that the mycelium colonises faster in the period where any surviving contams would be weakened by the "sterilisation" process? Once again just my thoughts, I'm still very much a noobie
-------------------- great write-up for new-guys ___________________________________________________________
  ____________________________________________________________ Cogsy
|
bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Cold House. Need HEAT PAD ASAP! [Re: Cogsy]
#19384160 - 01/07/14 06:13 PM (10 years, 23 days ago) |
|
|
after sterilization the contams that would be left would all be endospores? that take a longer time to germinate as well as being in so few numbers that the would easily get overran by mycelium perhaps.
I guess I really would just like to know a definitive answer but I don't think I'll get one
Edited by Trusted cuItivator (01/07/14 06:14 PM)
|
Cogsy
Psychedelic cultivator


Registered: 11/08/13
Posts: 265
Last seen: 7 years, 2 months
|
Re: Cold House. Need HEAT PAD ASAP! [Re: bodhisatta]
#19384177 - 01/07/14 06:18 PM (10 years, 23 days ago) |
|
|
It all seems to be very speculative :0 whatever works best for you probably is best for you
-------------------- great write-up for new-guys ___________________________________________________________
  ____________________________________________________________ Cogsy
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Cold House. Need HEAT PAD ASAP! [Re: bodhisatta]
#19384199 - 01/07/14 06:22 PM (10 years, 23 days ago) |
|
|
yes there would be bacteria left in your jar after "steriliztion" and many of the bacteia may lie dormant till higher temps are reached as far as i know
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Cold House. Need HEAT PAD ASAP! [Re: cronicr]
#19384219 - 01/07/14 06:26 PM (10 years, 23 days ago) |
|
|
I'm sure there are plenty of tardigrades in the jars
|
Cogsy
Psychedelic cultivator


Registered: 11/08/13
Posts: 265
Last seen: 7 years, 2 months
|
Re: Cold House. Need HEAT PAD ASAP! [Re: bodhisatta]
#19384243 - 01/07/14 06:30 PM (10 years, 23 days ago) |
|
|
Aren't tardigrades a type of extremeophile that feeds on mosses and lichen.?
-------------------- great write-up for new-guys ___________________________________________________________
  ____________________________________________________________ Cogsy
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
|
Re: Cold House. Need HEAT PAD ASAP! [Re: Cogsy]
#19384253 - 01/07/14 06:33 PM (10 years, 23 days ago) |
|
|
Let's not forget the fact that jar filters are not 100% effective, especially verm filters which shift when new growers feel a need to look at their jars twice a day. In an enclosed box, when one jar goes bad, the escaping mold spores easily get into the other jars via any imperfections in the filters. This is a big reason why people using incubators in the past had historically higher contamination rates than those who colonize at the same temperature on an open shelf.
Another recurring problem over the years with incubators of all designs is when the made in china thermostat in the fish tank heater sticks in the on position, the tank heats up enough to kill or weaken the mycelium. Thermophilic bacteria if present and some molds are favored by these high temperatures.
Heat doesn't magically cause mold spores to materialize but if they are there it will favor them over mushroom mycelium which doesn't appreciate being over about 80F, referring to the temperature inside the jar.
Further, regardless of how sterile you made the jar and filter, if you injected with spores from a spore print, I guarantee you also injected some mold and bacteria along with it regardless of who made the print and syringe. You want to favor your mushroom mycelium over these contaminants. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
Cogsy
Psychedelic cultivator


Registered: 11/08/13
Posts: 265
Last seen: 7 years, 2 months
|
Re: Cold House. Need HEAT PAD ASAP! [Re: RogerRabbit]
#19384313 - 01/07/14 06:41 PM (10 years, 23 days ago) |
|
|
And as I've said misuse of incubation is criminal now I struggle to keep my grow room in the 65-70 range and I've found that keeping a TiT around 80f (which in fairness you say is normal room temperature) increases colonisation times massively for me
-------------------- great write-up for new-guys ___________________________________________________________
  ____________________________________________________________ Cogsy
|
bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Cold House. Need HEAT PAD ASAP! [Re: Cogsy]
#19384393 - 01/07/14 06:56 PM (10 years, 23 days ago) |
|
|
Quote:
Cogsy said: Aren't tardigrades a type of extremeophile that feeds on mosses and lichen.?
yea I was just being funny since they survive anything
|
twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
|
Re: Cold House. Need HEAT PAD ASAP! [Re: RogerRabbit]
#19384425 - 01/07/14 07:01 PM (10 years, 23 days ago) |
|
|
Quote:
Cogsy said: And as I've said misuse of incubation is criminal now I struggle to keep my grow room in the 65-70 range and I've found that keeping a TiT around 80f (which in fairness you say is normal room temperature) increases colonisation times massively for me 
as RR said above how do you know your TIT is at 80F do you have a seperate thermometer to monitor your water also? when i made my TIT i used an aquarium heater and a thermostat and had a probe from a thermometer ran into the TIT just to make sure everything was on the same page. im not leaving a fish tank heater on while im not at the house either especially when its not in a fish tank. my 
Quote:
RogerRabbit said: Let's not forget the fact that jar filters are not 100% effective, especially verm filters which shift when new growers feel a need to look at their jars twice a day. In an enclosed box, when one jar goes bad, the escaping mold spores easily get into the other jars via any imperfections in the filters. This is a big reason why people using incubators in the past had historically higher contamination rates than those who colonize at the same temperature on an open shelf.
Another recurring problem over the years with incubators of all designs is when the made in china thermostat in the fish tank heater sticks in the on position, the tank heats up enough to kill or weaken the mycelium. Thermophilic bacteria if present and some molds are favored by these high temperatures.
RR
Edited by twistedty (01/07/14 07:01 PM)
|
|