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OfflineCarlWonderful
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Temp and Spawn Jars & MS questions
    #19379863 - 01/06/14 10:25 PM (10 years, 1 month ago)

Is there any disadvantage in keeping spawn jars at colder temperatures (say 60, or 50 F) other than just taking longer to colonize? 

Quick Q -(Also is temp fluctuation during spawn running a problem like it is during colonizing and fruiting? :confused:)


So is a lower temp detrimental to the myc during spawn run?.  I was wondering if the slowing temps could be used to an advantage say in MS grows where you plan on running alot of myc G2G but wanted to wait to see what kind of results you had before you start G2G'ing lots of jars.


So basically you would be "stalling" some of your first jars while you see what kind of results you got. If the results are good you would bring the temps back up and continue fruiting and G2G's.
Would this make sense or with MS are you just gonna have so many strains going on all over that the first fruits wouldn't necessarily be setting the tone for the future flushes and grows? 

hope that made sense..:smirk:


Edited by CarlWonderful (01/07/14 01:48 PM)


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OfflinecronicrFacebook
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Re: Temp and Spawn Jars [Re: CarlWonderful]
    #19379909 - 01/06/14 10:33 PM (10 years, 1 month ago)

quick A: temp flux are fine
no lower temps don't do any harm just slow it down, i was just given a bunch of jars that were colonizing in pretty low temps



the ape in the first pic and the crs in the second were colonized at temps probly around or below60, you can see the jar in the middle is mine that colonized at about 74 how much thicker the growth is, after 4 days at my house and a g2g with the crs


you can see how the myc thickened right up and here they are almost at full colonization


they were about 2 months at those cold temps though just for the record lol


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OfflineCarlWonderful
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Re: Temp and Spawn Jars [Re: cronicr]
    #19382553 - 01/07/14 01:48 PM (10 years, 1 month ago)

So it looks like they bounced right back with no problem. Wonder if you'll notice any negative effects down the road due to the low temps and fluctuation during spawn run..?  :shrug:

Any comments on the MS questions?  Say you go MS to 4 jars, then g2g from these 4, say to 30 or something, are you going to see different "sub strains" (not sure if this is the correct term here) throughout all the jars and even each flush...Is there any consistency with MS or is it just a constant lottery from start to finish, from flush to flush? :confused: 


Thinking MS to 4 jars at the start... each of these 4 jars will have its own mix of genetics all competing, eventually you might end up with half a dozen all growing intertwined? (Is that a good ballpark, 6 or so or does it wind down to less usually once introduced to a bulk and colonized?) I know there might not be an actual answer to these questions but just thinking out loud I guess.. :blush:

If this is true than can you even base anything on the first fruits you end up seeing from MS grow or is each flush most likely to be a completely different "sub strain?":eek: (sorry again)


Edited by CarlWonderful (01/07/14 01:49 PM)


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OfflinecronicrFacebook
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Re: Temp and Spawn Jars [Re: CarlWonderful]
    #19382592 - 01/07/14 01:54 PM (10 years, 1 month ago)

Quote:

CarlWonderful said:
So it looks like they bounced right back with no problem. Wonder if you'll notice any negative effects down the road due to the low temps and fluctuation during spawn run..?  :shrug:

Any comments on the MS questions?  Say you go MS to 4 jars, then g2g from these 4, say to 30 or something, are you going to see different "sub strains" (not sure if this is the correct term here) throughout all the jars and even each flush...Is there any consistency with MS or is it just a constant lottery from start to finish, from flush to flush? :confused: 






yes you will it's a constant lottery:thumbup:

and yes every fruit will be it's own strain and could be made up of multiple genetics, not always a bad thing though, i've seen many of my ms grows outdo my iso's no problem, if you get a substrate colonized and give it proper conditions it will fruit with lots of fruits thats just what cubes do:cool:


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OfflineCarlWonderful
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Re: Temp and Spawn Jars [Re: cronicr]
    #19382646 - 01/07/14 02:06 PM (10 years, 1 month ago)

Ok, thought so..

Trying to really understand how this works, I apologize if these are impossible questions..
Lets say you just have 1 MS jar. You g2g this into 10 jars.  Are all 10 jars going to have about the same makeup, contain the same strains as the master jar or it is just a total orgy cluster%$#% going on everywhere? I guess I mean are some strains or substrains going to thrive in some of the jars while others thrive in other jars. Could you have great 1st flushes and terrible 2nd's great 3rds.  If there anything to go by or just cross your fingers and wait to be surprizes each flush? :shrug:


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Re: Temp and Spawn Jars [Re: CarlWonderful]
    #19382825 - 01/07/14 02:37 PM (10 years, 1 month ago)

just wait for your prize:thumbup: but if you give it optimal conditions you should for the most part be able to pull 80% of your total yield from your first 2 flushes regardless.
and if you g2g to 10 jars you now have 10 jars that have 10 different possibility's for hundreds of different strains which will change with each flush


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It doesn't matter what i think of you...all that matters is clean spawn

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OfflineCarlWonderful
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Re: Temp and Spawn Jars [Re: cronicr]
    #19384785 - 01/07/14 08:07 PM (10 years, 1 month ago)

So your hoping to get something good early on so you can clone a winner and G2G the clone out for 3 or 4 transfers..



Whats a good limit on age of mycelium from Ms to choose a clone.  I mean at what point (assuming you haven't hit the lottery  :supercrankey: with anything worth cloning) would you just start over with another MS and hope cross you fingers again?  :takeatissue:


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Re: Temp and Spawn Jars [Re: CarlWonderful]
    #19384845 - 01/07/14 08:19 PM (10 years, 1 month ago)

yep i like to try clone from flush one and test it out, i find the further into flushes i get the more sectoring that happens


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It doesn't matter what i think of you...all that matters is clean spawn

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OfflineCarlWonderful
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Re: Temp and Spawn Jars [Re: cronicr]
    #19384915 - 01/07/14 08:32 PM (10 years, 1 month ago)

How are you cloning...

-Pins on first flush?
-Tissue from almost mature fruit?

And how are you testing..

-Waiting to sample the flush before you start running the myc form the clone or what makes the most sense.

If you go tissue to agar can you stall it or wait til you get to test the first flush before you waste your time (assuming your not happy with the clone)?


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Re: Temp and Spawn Jars [Re: CarlWonderful]
    #19384938 - 01/07/14 08:37 PM (10 years, 1 month ago)

i like pins to plates, check for sectoring if it sectors you get the choice of isolating sectors which will need to be tested individually or take samples from each sector and hope for the best, if it doesn't sector too much i make one quick transfer to make sure it's clean then off to grain to fruit and if i'm happy i go back to the plate i started with and put it to a slant for storage, i simply put my plate in the fridge to slow growth while i await results


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineCarlWonderful
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Re: Temp and Spawn Jars [Re: cronicr]
    #19385052 - 01/07/14 09:00 PM (10 years, 1 month ago)

Thank you, that is a great run down. 

Do you ever just test out the fruits from that first flush to determine if its a keeper?  Or maybe that doesn't make sense because you might have more than one strain fruiting in any flush (unless you used a isolate)...Right?


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Re: Temp and Spawn Jars [Re: CarlWonderful]
    #19385082 - 01/07/14 09:06 PM (10 years, 1 month ago)

for potency?


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OfflineCarlWonderful
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Re: Temp and Spawn Jars [Re: cronicr]
    #19385133 - 01/07/14 09:15 PM (10 years, 1 month ago)

Whatever traits your looking for.  Can you just test out the first flush (say 3 days or so after the pin was taken) and determine if the clones gonna be good? Or are there to many strains (or sub strains, right term???) possibly growing in that first flush?

does that make sense?


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Re: Temp and Spawn Jars [Re: CarlWonderful]
    #19385164 - 01/07/14 09:22 PM (10 years, 1 month ago)

oh okay thats another one all together and sorta a problem with cloning a pin is thatyou don't see it mature but the fact that it was the first to pop is a good trait to start, especially if it's a cluster but yes the rest of the fruits won't really tell you anything becasue there diffrent geneticly


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It doesn't matter what i think of you...all that matters is clean spawn

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OfflineCarlWonderful
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Re: Temp and Spawn Jars [Re: cronicr]
    #19385224 - 01/07/14 09:32 PM (10 years, 1 month ago)

Gotcha.  What is it about clusters that make them better candidates for cloning?


Could you tell me why you prefer pins over a mature fruit that you can actually get a look at?:shrug:


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Re: Temp and Spawn Jars [Re: CarlWonderful]
    #19385236 - 01/07/14 09:33 PM (10 years, 1 month ago)

pins are faster then mature fruits and as for clusters 10 is always better then 1:wink:


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Re: Temp and Spawn Jars [Re: cronicr]
    #19385259 - 01/07/14 09:37 PM (10 years, 1 month ago)

single fruit cloned

cluster cloned


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It doesn't matter what i think of you...all that matters is clean spawn

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Invisibleblindingleaf
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Re: Temp and Spawn Jars [Re: cronicr]
    #19385335 - 01/07/14 09:52 PM (10 years, 1 month ago)

woa thats a great comparison photo!  never seen one before.
in the summer when i started, i liked the idea of cloning single fruits, i think they are easier to pick (tear up sub less if u doing twist and pull) , but as i kept reading the forums, i t was constantly mentioned that one should clone from clusters, and that photo is a good example of why


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MICROBIAL HUSBANDRY!!!!

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Re: Temp and Spawn Jars [Re: blindingleaf]
    #19385461 - 01/07/14 10:11 PM (10 years, 1 month ago)

oddly enough the yield was never effected, the single fruit clone just produced a better second flush


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Invisibleblindingleaf
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Re: Temp and Spawn Jars [Re: cronicr]
    #19386250 - 01/08/14 01:31 AM (10 years, 1 month ago)

i remember reading somewhere that someone cloned a fruit that when mature, fell down, like a tree, which made harvest easier.  it may even have been an RR quote, but I'm not sure.  or maybe a dream, because it seems more substrate based?  i dunno tho, I'm still learning..


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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