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Mosey3012



Registered: 12/12/12
Posts: 675
Last seen: 3 months, 15 days
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Pushing Week 4... No Pins... Post Pics just to hear thoughts
#19382260 - 01/07/14 12:36 PM (10 years, 23 days ago) |
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So I used Rye Grass Seed as my spawn and coir/verm for my sub. *I'd like to add that when prepping the seed everything seemed to work out fine but there was a bit of clumping in some of the jars after being PC'd, however this wasn't an issue at all as far as I could tell as the jars fully colonized at a constant rate, even after a shake or 2.
I used clean agar wedges to inoculate the spawn.
Each tray gets 2 quarts spawn and 4 quarts of sub, so 1:2 ratio.
It was left to fully colonize for 10 days (which on inspection of the top was completely white, then given another 2 or 3 days to do its thing just in case there were some areas below the surface that needed a little more time.
The sub was properly pasteurized using franks method.
On 1/9/14 (Thursday) this tray will have been sitting in fruiting conditions for 30 days... At first I simply slid it into the mono where I had some pp5 containers fruiting already and left it alone for 10 days... After 10 days I was starting to wonder wtf was going on so a couple times a day I would lightly mist into the box, just enough to where the surface of the sub was covered with very very tiny little water blobs. I did this because I noticed that (from what I could see with the naked eye) there wasn't really much moisture on the surface ever and figured well shit maybe the necessary evaporation isn't occurring and decided this would help. The "mist layer" on the sub would usually evaporated within 3-4 hours it seems so I'd keep a healthy cycle of that going for another 10 days. At day 20 having tried all this I just wasn't sure what to do and have just been trying to be patient. There have been no signs of metabolite or contam anywhere... Its just chillin...



Anywho, I was planning on stripping the plastic off and dunking it for like 12-24 hours thursday night if nothing happens by then and then throw it back in the box to see if anything changes. I've accepted that this is probably a dud... along with the one running about a week behind this one which is doing the same thing. I have another mono built EXACTLY the same sitting right next to this one that has pp5's fruiting just fine so its not environmental I dont personally think. RH stays at 99%+ constantly and the temps in here run at an average 70-73 constantly.
-------------------- "Each of these lives is the right one! Every path is the right path. Everything could have been anything else and it would have just as much meaning"
  "All that is gold does not glitter, Not all those who wander are lost"
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urthtown
meat popsicle



Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
Last seen: 2 years, 4 months
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Re: Pushing Week 4... No Pins... Post Pics just to hear thoughts [Re: Mosey3012]
#19382278 - 01/07/14 12:43 PM (10 years, 23 days ago) |
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is this a MS on agar or an isolate or clone?
-------------------- Cluster Headache sufferer? Cluster Busting Veil Tear GIF Flower Pot Grow GIF Mini Mono Tub GIFS "All mushrooms are edible, but some only once." -- Croatian Proverb
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BigGreenMat
Stranger

Registered: 11/04/13
Posts: 161
Last seen: 3 years, 3 months
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Re: Pushing Week 4... No Pins... Post Pics just to hear thoughts [Re: Mosey3012]
#19382293 - 01/07/14 12:47 PM (10 years, 23 days ago) |
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I have no answers for you but figured I would commiserate. It is maddening when it looks so good and does so little. I have the same game going in a few different ways.
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Us.Two
The Innovator


Registered: 01/29/12 
Posts: 106
Last seen: 3 years, 4 months
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Re: Pushing Week 4... No Pins... Post Pics just to hear thoughts [Re: BigGreenMat]
#19382339 - 01/07/14 01:01 PM (10 years, 23 days ago) |
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You said it's fruiting in a monotub? Is it a monotub setup or not? To me it looks as if the mycelium doesn't know it's time to fruit, aka the conditions are more suited to mycelium growth instead of fruiting. And according to what I see and you've told us I think it's an FAE issue.
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Mosey3012



Registered: 12/12/12
Posts: 675
Last seen: 3 months, 15 days
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Re: Pushing Week 4... No Pins... Post Pics just to hear thoughts [Re: Us.Two]
#19382579 - 01/07/14 01:52 PM (10 years, 23 days ago) |
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Quote:
Us.Two said: You said it's fruiting in a monotub? Is it a monotub setup or not? To me it looks as if the mycelium doesn't know it's time to fruit, aka the conditions are more suited to mycelium growth instead of fruiting. And according to what I see and you've told us I think it's an FAE issue.
Yes I am fruiting my trays in a monotub, built just like your average mono would be built ( 2 holes on each long side near sub surface, a hole on each short side near the top center). 2 of these silver trays fits in a 66qt mono and takes up ALMOST as much subspace as what a single flat block would however due to still getting random contams in some of my "bulk" attempts I've decided to do it this way so that if something does go bad its literally half as bad as loosing a whole tub. I may be sacrificing yield some but my main concern is getting a more consistent success rate using bulk subs and this was the most logical way for me to do so.
I switched from bucket pasteurization (I assumed this was 1 of my main issues for contam in my trays in the past because I know I always use good clean spawn) to a proper stove top pasteurization recently. This was 1 of the reasons i mentioned the clumpyness of the grains. Although everything colonized just fine I found myself questioning if I under or even over hydrated the seeds during their prep and perhaps they were 2 wet when I inoc'd them, or 2 dry... or hell i donno, it makes more sense in my mind to fear they were 2 wet since i assume if they were 2 dry the myc would have taken forever to colonize the jars... If the grain was a bit wetter then it should have been could that have caused a potential bactieral contam that might be stopping this from fruiting?
I know for sure its not an FAE or RH issue. I have a very reliable hygrometer I monitor RH with and as I said in the post there's another tub built literally the same way sitting right next to it with pp5 containers that are starting to fruit now just fine. This would at least indicate to me that there is sufficient FAE occurring where and how I have them sitting. I also feel comfortable saying this as this is the same mono/spot/environmental conditions I've fruited multiple other batches in with no issues all of last year
-------------------- "Each of these lives is the right one! Every path is the right path. Everything could have been anything else and it would have just as much meaning"
  "All that is gold does not glitter, Not all those who wander are lost"
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Us.Two
The Innovator


Registered: 01/29/12 
Posts: 106
Last seen: 3 years, 4 months
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Re: Pushing Week 4... No Pins... Post Pics just to hear thoughts [Re: Mosey3012]
#19385615 - 01/07/14 10:42 PM (10 years, 23 days ago) |
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Hmmmm, I understand where you're coming from but I truly believe it is an air exchange problem. Mushrooms inherently know what to do and when to do it by certain environmental "triggers", and I see mycelium growth on your substrate. Whatever you're using to fill the holes could be plugged, so I would suggest fruiting your casings in a SGFC to almost guarantee FAE. Or you could try replugging the holes a little looser. Also, did you use rye grass seed with your other cases?
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Mosey3012



Registered: 12/12/12
Posts: 675
Last seen: 3 months, 15 days
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Re: Pushing Week 4... No Pins... Post Pics just to hear thoughts [Re: Us.Two]
#19387729 - 01/08/14 11:50 AM (10 years, 22 days ago) |
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Quote:
urthtown said: is this a MS on agar or an isolate or clone?
Ummm this is a good question. The original culture was sent to me on agar that I had to clean up but I dont remember ever asking if it originated from MS or clone. On the other hand I have cloned one of the fruits that grew from a last batch but fuck if I can remember which plate I used at this point its been a month lol, ive had so much else going on. May need to take my journal back out. I'd like to assume the original culture came from a clone...
Quote:
Us.Two said: Hmmmm, I understand where you're coming from but I truly believe it is an air exchange problem. Mushrooms inherently know what to do and when to do it by certain environmental "triggers", and I see mycelium growth on your substrate. Whatever you're using to fill the holes could be plugged, so I would suggest fruiting your casings in a SGFC to almost guarantee FAE. Or you could try replugging the holes a little looser. Also, did you use rye grass seed with your other cases?
Everything I have going right now comes from the same original master culture and was all put onto rye grass seed for this trial. As I said above I cleaned up a plate that was sent to me so I had 2 or 3 different clean cultures growing out that I was using. This particular round of stuff all came off the same plate of agar though.
I guess I'll look at changing around the environmental conditions some to see what that does. I just cant understand how a box right next to it has pp5's that are starting to fruit just fine from the exact same agar and grain as what I used for the trays. Go figure.
-------------------- "Each of these lives is the right one! Every path is the right path. Everything could have been anything else and it would have just as much meaning"
  "All that is gold does not glitter, Not all those who wander are lost"
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