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Invisibletryptonite
mushroom ninja
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Registered: 02/06/04
Posts: 931
Loc: aussie
Subbalteatus Grow Log
    #18817852 - 09/09/13 05:18 AM (10 years, 5 months ago)

Plan
Spore print
    V
Malt agar plates -> incubate 28oC until plate 2/3 grown
    V
Subculture -> incubate 28oC until good mycelium is isolated
    V
Whole Rye Grain jars -> incubate 28oC until full colonisation
    V
H-Dung/Coir/Verm trays -> incubate 28oC, 90% RH until full colonisation
    V
Case Trays 1 inch with peat based soil -> incubate 25oC, 95% RH, with fluro light and fresh air exchange until pin formation
    V
Cropping -> incubate 25oC, 90% RH, with fluro light and fresh air exchange until harvest is ready
    V
HARVEST -> Sample

Grow Log starts

05/09

Plates inoculated by aseptic scalpel scraping of a 2011, not properly sealed, non-sterile, wild spore-print from printing paper onto x10 malt agar plates. All plates were placed in an incubator set to 28oC. After a couple days noticed a sudden growth on one of the plates.

09/09

Removed buildup of excess condensate from lid of all plates by holding upside down, removing lid and turning it the right way up so the water fell then quickly replaced on petri.

Noticed new growth starting on x1 plate from P1 and x3 plates from P2 group. One plate from the p1 group I suspect to be a contaminated due to sudden growth after 2 days, other plates took 4 to start. Also the appearance is much different to the cottony-type mycelium this species has.


inoculated plates


suspected contaminated plate - disposed of


successful plate inoculation is evident in x4 plates so far


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Offlinemoricz
Inky Cap Cultivator
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Registered: 07/14/13
Posts: 969
Loc: EU
Last seen: 4 months, 15 days
Trusted Cultivator
Re: Subbalteatus Grow Log [Re: tryptonite]
    #19381071 - 01/07/14 06:05 AM (10 years, 1 month ago)

Hi, nice plates, interested in your grow atempt, is it workin? Any progress? Or dropped the idea.

Peace


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