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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
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Re: Chaga fruiting on the plate! [Re: SnowArcher]
#19373982 - 01/05/14 05:32 PM (10 years, 1 month ago) |
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All three plates are the same. I am honestly a little bummed that we didn't sub it before it started dropping spores. I was out of town and my partner saw so enthralled watching it he didn't think about the spores contaminating the isolate. It might not really matter since we are gonna try to o o grain then to four inch dowels to put into live birches.
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SnowArcher
Hood Rat


Registered: 12/30/13
Posts: 179
Last seen: 2 years, 11 months
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It is too bad that you didn't catch it but am sure it will be fine - let us know!
Have you inoculated live birch before? I have considered hitting a few of the trees around here for a personal source but am nervous that I will just end up harming some trees with no fruit...
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nanncee



Registered: 12/01/12
Posts: 434
Loc: Utah
Last seen: 9 years, 2 months
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Re: Chaga fruiting on the plate! [Re: SnowArcher]
#19377025 - 01/06/14 11:57 AM (10 years, 1 month ago) |
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Nothing to add (everyone said what I would have suggested) but man that is badass! Makes me want to track down a culture of chaga.
-------------------- I am a small scale farmer, come check out what we do. www.facebook.com/biocentricbros Check out our Youtube videos. www.youtube.com/biocentricbros
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deadmandave
Slime


Registered: 02/16/10
Posts: 3,368
Loc:
Last seen: 1 day, 23 hours
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perhaps you cant pluck one of the fruits, open it up and take a culture from inside the chaga, much like culturing a wild found specimen.
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
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Re: Chaga fruiting on the plate! [Re: SnowArcher]
#19378015 - 01/06/14 04:31 PM (10 years, 1 month ago) |
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Quote:
SnowArcher said: It is too bad that you didn't catch it but am sure it will be fine - let us know!
Have you inoculated live birch before? I have considered hitting a few of the trees around here for a personal source but am nervous that I will just end up harming some trees with no fruit...
Birch is a weed tree on my farm. We have 80 acres of them. So plenty to experiment with
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 19 days
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Quote:
Amanita virosa said: It might not really matter since we are gonna try to o o grain then to four inch dowels to put into live birches.
I am trying to do this as well, but have never made dowels or inoculated trees/logs. Is there any reason you're using 4 inch dowels? I thought they were usually smaller? I'm really clueless when it comes to this type of growing... Also, how do you colonize the dowels, just with a wedge in a ball jar, or...?
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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forrest



Registered: 11/16/12
Posts: 1,011
Loc: The Netherlands
Last seen: 4 years, 7 months
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Re: Chaga fruiting on the plate! [Re: Forrester]
#19380019 - 01/06/14 10:56 PM (10 years, 1 month ago) |
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you can just throw a bunch of dowels with some grains in a jar and treat it as a normal spawn jar except maybe longer incubation.
but for logs i think it's better so inoculate with sawdust, either by cutting ''>'' out of them or in between logs on the ends (sawdust sandwiched), it goes faster, less expensive, less chance of failure.
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 19 days
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Re: Chaga fruiting on the plate! [Re: forrest]
#19380057 - 01/06/14 11:04 PM (10 years, 1 month ago) |
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Quote:
forrest said: you can just throw a bunch of dowels with some grains in a jar and treat it as a normal spawn jar except maybe longer incubation.
but for logs i think it's better so inoculate with sawdust, either by cutting ''>'' out of them or in between logs on the ends (sawdust sandwiched), it goes faster, less expensive, less chance of failure.
Sounds good for the dowels - but we're talking live trees here, not logs so no sandwiching opportunities!
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
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Re: Chaga fruiting on the plate! [Re: Forrester] 1
#19381578 - 01/07/14 09:52 AM (10 years, 1 month ago) |
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Quote:
Forrester said:
Quote:
Amanita virosa said: It might not really matter since we are gonna try to o o grain then to four inch dowels to put into live birches.
I am trying to do this as well, but have never made dowels or inoculated trees/logs. Is there any reason you're using 4 inch dowels? I thought they were usually smaller? I'm really clueless when it comes to this type of growing... Also, how do you colonize the dowels, just with a wedge in a ball jar, or...?
 just use grain and hydrate the dowels by soaking them in coffee water. we soak for a couple of days, then bring to boil, steam dry, and then autoclave for one hour. then inoculate with grain. We sold 50,000 plus plugs last year. The usual size plug is 1 inch by 5/16th dia. But for spiking live trees we are going with something much longer.
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SnowArcher
Hood Rat


Registered: 12/30/13
Posts: 179
Last seen: 2 years, 11 months
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How many dowels are you putting into live trees? Do you think the strain would translate well to aspens?
Nice page! Got you on my list for future cultures.
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 19 days
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Quote:
Amanita virosa said: just use grain and hydrate the dowels by soaking them in coffee water. we soak for a couple of days, then bring to boil, steam dry, and then autoclave for one hour. then inoculate with grain. We sold 50,000 plus plugs last year. The usual size plug is 1 inch by 5/16th dia. But for spiking live trees we are going with something much longer.
Awesome man that's exactly what I was looking for, thanks so much for the info!
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
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Re: Chaga fruiting on the plate! [Re: Forrester]
#19389638 - 01/08/14 05:48 PM (10 years, 1 month ago) |
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Quote:
Forrester said:
Quote:
Amanita virosa said: just use grain and hydrate the dowels by soaking them in coffee water. we soak for a couple of days, then bring to boil, steam dry, and then autoclave for one hour. then inoculate with grain. We sold 50,000 plus plugs last year. The usual size plug is 1 inch by 5/16th dia. But for spiking live trees we are going with something much longer.
Awesome man that's exactly what I was looking for, thanks so much for the info! 
Two other notes. Make sure you use spiral groove dowels and as you heat them up keep checking them. You are shooting for 25 percent sinking. In other words don't over hydrate then. For your first batch disk 24 hrs then boil. Sample periodically by scooping some out and seeing what percent sink. Be SURE you let them steam completely dry too. If they are too wet when you clave them the water comes out and settles in the bottom of the bag. Not good
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 19 days
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Quote:
Amanita virosa said: Two other notes. Make sure you use spiral groove dowels and as you heat them up keep checking them. You are shooting for 25 percent sinking. In other words don't over hydrate then. For your first batch disk 24 hrs then boil. Sample periodically by scooping some out and seeing what percent sink. Be SURE you let them steam completely dry too. If they are too wet when you clave them the water comes out and settles in the bottom of the bag. Not good
Kinda confused on the sinking thing... so I just take a bunch out of the boiling water and toss them into a different pot of cold water to see how many sink?
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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FrayedO
Newbie


Registered: 02/23/06 
Posts: 9
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Do you still have this culture? I am looking to trade for a Reishi culture, or something, as I am interested into doing an experiment inoculating live birch trees.
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