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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: cooling substrate quickly [Re: cronicr]
    #19380379 - 01/07/14 12:39 AM (10 years, 24 days ago)



--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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InvisibleMarcusFreeman
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Registered: 09/16/13
Posts: 376
Re: cooling substrate quickly [Re: FrankHorrigan]
    #19380515 - 01/07/14 01:22 AM (10 years, 24 days ago)

Quote:

FrankHorrigan said:
Quote:

MarcusFreeman said:
bacteria and contams are all over your jar the second you open that PC.




Yes, the outside of the jar. That is why we use filters for our sterile media :shake:





Well yeah, :smile:. but even when the jar is knocked up, there are bound to be a very small amount of contams that get in. The mycelium out numbers and out competes the competitors and the jar is happy.... Well in most people's jars. :thumbup:


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
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Last seen: 17 days, 12 hours
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Re: cooling substrate quickly [Re: MarcusFreeman]
    #19380537 - 01/07/14 01:30 AM (10 years, 24 days ago)

If there are any contams present that the mycellium must "fight" you already lost the battle.

Inoculating should not introduce any kind of contamination, if it does you are doing something wrong.

This is not a race against contams.....


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InvisibleMarcusFreeman
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Registered: 09/16/13
Posts: 376
Re: cooling substrate quickly [Re: PussyFart]
    #19380548 - 01/07/14 01:32 AM (10 years, 24 days ago)

I was under the impression that when using multispore from a print, there is a good chance some sort of contam could get in. But generally an irrelevant amount. Or is that wrong?


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



Edited by MarcusFreeman (01/07/14 01:32 AM)


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
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Re: cooling substrate quickly [Re: MarcusFreeman]
    #19380564 - 01/07/14 01:35 AM (10 years, 24 days ago)

when your starting from a syringe there's a chance it's not clean but thats your risk, use agar and there's no race no dumb luck:thumbup:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: cooling substrate quickly [Re: bigd17]
    #19380961 - 01/07/14 05:07 AM (10 years, 24 days ago)

Quote:

bigd17 said:
Perhaps one could submerge the pot in a sink of cold water and ice maybe just halfway up the sides of the pot. 

Put something heavy on the lid to hold it down.

It's the same method for cooling big pots of soups or sauces before putting them in the fridge to chill.

Of course the reason I ask is if someone else has already thought of this and tried it and ended up getting contaminants due to this then obviously it wouldn't be worth wasting the time to even try.




Horrible idea.

Allow the substrates to cool slowly to avoid damaging the filters by the sudden rush of air and/or loss of pressure.  It's sterile inside the pressure cooker and jars until you open them.  Allow to cool slowly for 12 hours or so until you can handle the jars without protection for your hands from the heat.

Remember, we're sterilizing, not cooking a soup.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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OfflinePistil-Whipped
Registered: 12/26/13
Posts: 109
Last seen: 5 years, 21 days
Re: cooling substrate quickly [Re: MarcusFreeman]
    #19381121 - 01/07/14 06:38 AM (10 years, 24 days ago)

Not trying to be contrary here but taxes don't pay for the postal service.  All revenue for the post office is generated through stamps/shipping costs.   


Edit:  I read through this post too quickly and thought the discussion was on pasteurizing, not sterilizing.    It's still very early here.  COFFEE.


Edited by Pistil-Whipped (01/07/14 06:41 AM)


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Offlinebigd17
Say Budget To Me Again...
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Registered: 07/31/08
Posts: 334
Loc: Dexter's Laboratory Flag
Last seen: 3 years, 7 months
Re: cooling substrate quickly [Re: Pistil-Whipped]
    #19381440 - 01/07/14 09:15 AM (10 years, 23 days ago)

Cool, I haven't been able to find micropore tape thus far but I found breathable paper tape that was in the pharm section.  I just put a piece over each noc point and put another over it after I injected.

I hope that wasn't a bad idea since the second piece of tape obviously wasn't sterilized.

I also don't have an FH but intend on trying to build on in the distant future, until then I do need to make a glovebox.

I know most people are probably gonna slap their foreheads but I opted instead to turn the a/c off, clean my entire work area with bleach water solution, and just do them inside of a sterilite container that I wiped down with bleach solution.

I know it's not perfect but until I build a GB it's the best I got.  Plus we keep our house pretty clean.

In retrospect, I could prob just cut holes in the side of that bitch so I can work with the lid on, I mean it's not a true GB but until I find tyvek sleeves and buy the silicone and pvc its a hell of a lot better than working in basically open air.


--------------------
"The secret to success is to make your Vocation a Vacation."
      -Mark Twain

Confucius say,"man who stand on toilet, high on pot."

The answer to the question never asked is always no.


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