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Offlinehighc
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frozen grains
    #19378611 - 01/06/14 06:33 PM (10 years, 24 days ago)

So I obtained some pretty sterilized grain jars. During shipping some of them got pretty chilly and froze in spots. Should I sit them at room temp for a while before knocking up?

A search yielded little. Most people were saying their colonized  jars had frozen. These haven't been inoculated yet.


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Re: frozen grains [Re: highc]
    #19378618 - 01/06/14 06:34 PM (10 years, 24 days ago)

that is an odd one,but i would waitfor room temp for sure


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Offlineitsaconspiracy
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Re: frozen grains [Re: highc]
    #19378629 - 01/06/14 06:36 PM (10 years, 24 days ago)

probably just let them thaw out and inoculate ,but i would re PC them just to make sure they are fully sterilized:thumbup: one question why didn't you make you own?


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Edited by itsaconspiracy (01/06/14 06:36 PM)


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Offlinehighc
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Re: frozen grains [Re: itsaconspiracy]
    #19378657 - 01/06/14 06:41 PM (10 years, 24 days ago)

Quote:

itsaconspiracy said:
probably just let them thaw out and inoculate ,but i would re PC them just to make sure they are fully sterilized:thumbup: one question why didn't you make you own?




I usually do. The holidays kind of drained me by the time my pressure cooker went up. That will set me back about 3 or 400 until I'm able to get another one in a month or two. I need to get 2014 rolling though so I got some from a vendor.


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Re: frozen grains [Re: highc]
    #19378667 - 01/06/14 06:43 PM (10 years, 24 days ago)

just steam it for an hour:thumbup: it's already been through the pc cycle so that should cut it no problem


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Offlineitsaconspiracy
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Re: frozen grains [Re: highc]
    #19378700 - 01/06/14 06:48 PM (10 years, 24 days ago)

damn dude that sucks which one are you getting thats so darn expensive mine only costed $60? (presto 16 quart and fits 7 quart jars). Plus :whathesaid: that'll work too.


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OfflineSgt. Pepper
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Re: frozen grains [Re: itsaconspiracy]
    #19378743 - 01/06/14 06:57 PM (10 years, 24 days ago)

I agree, just steam them for now, but wait for the glass to warm up so they don't shatter. Also, I love the emperor scorpion in your sig. Is that yours?


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Offlinehighc
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Re: frozen grains [Re: Sgt. Pepper]
    #19378788 - 01/06/14 07:05 PM (10 years, 24 days ago)

Quote:

itsaconspiracy said:
damn dude that sucks which one are you getting thats so darn expensive mine only costed $60? (presto 16 quart and fits 7 quart jars). Plus :whathesaid: that'll work too.




2 all american 30quart cookers. O yea I need that much!

Quote:

Sgt. Pepper said:
I agree, just steam them for now, but wait for the glass to warm up so they don't shatter. Also, I love the emperor scorpion in your sig. Is that yours?




Cool cool. And yes that's one of them.
Thats Dex. Stalker is in another cage. He's a death stalker and extremely dangerous.


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OfflineSgt. Pepper
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Re: frozen grains [Re: highc]
    #19378847 - 01/06/14 07:15 PM (10 years, 24 days ago)

Nice, that's a death stalker? I could swear that looks like an emperor. I've considered buying a death stalker, but then I think about my ferret sneaking out of his cage and knocking over the death stalker cage, releasing a horrible venomous tiny arachnid in my room to kill my darling ferret and myself. Maybe one day...

Back to mushrooms now, if you need to start pressure cooking quick why don't you get a cheap 16 quart presto until you have the money for a nice all American. I'd prefer that over buying premade jars anyday. And I've used nothing but a 16 quart presto and it gets the job done, albeit less efficiently than a large all american.


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Offlinehighc
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Re: frozen grains [Re: Sgt. Pepper]
    #19378856 - 01/06/14 07:17 PM (10 years, 24 days ago)

The black one is emperor yes.

Yeah I just may have to get a smaller one ATM.


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OfflineGod Stamet
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Re: frozen grains [Re: highc]
    #19378878 - 01/06/14 07:21 PM (10 years, 24 days ago)

it's colder than mars outside my house right now. fucking -38 with wind chill! just a little side note lol. I agree, just steam them and let them cool, I'm sure they're already sterile.


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OfflineAmanita virosa
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Re: frozen grains [Re: highc]
    #19378892 - 01/06/14 07:24 PM (10 years, 24 days ago)

What does the grain freezing have to do with sterility?  You weren't going to steam them if they had not frozen were ya? I would just knock them up.


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Re: frozen grains [Re: God Stamet]
    #19378900 - 01/06/14 07:25 PM (10 years, 24 days ago)

I actually have a gravid desert hairy scorpion at the moment, I doubt she won't eat them, but it's still interesting. And a 16 quart really isn't that bad. You can get several dozen done in a couple days if you stay up late.


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OfflinecronicrFacebook
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Re: frozen grains [Re: Amanita virosa]
    #19378911 - 01/06/14 07:27 PM (10 years, 24 days ago)

Quote:

Amanita virosa said:
What does the grain freezing have to do with sterility?  You weren't going to steam them if they had not frozen were ya? I would just knock them up.



i was just saying to steam them if he was worried they weren't sterile, put a frozen jar in hot water it will break


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Re: frozen grains [Re: cronicr]
    #19379079 - 01/06/14 07:59 PM (10 years, 24 days ago)

We're worried about sterility since it is a presterilized jar, not cause it's cold. Who knows how long it's been sitting around for, an hour of steam sterilizing is just a good safety measure.


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Offlineitsaconspiracy
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Re: frozen grains [Re: Sgt. Pepper]
    #19379656 - 01/06/14 09:50 PM (10 years, 24 days ago)

Quote:

Sgt. Pepper said:
And a 16 quart really isn't that bad. You can get several dozen done in a couple days if you stay up late.



so far i've only done 14 grain jars in one day ,but probably could do 21:shrug:
Quote:

highc said:
2 all american 30quart cookers. O yea I need that much!


:gonnaneedmyglasses: whoa if you need that much post more grows:lol:


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Re: frozen grains [Re: itsaconspiracy]
    #19379816 - 01/06/14 10:18 PM (10 years, 24 days ago)

Quote:

itsaconspiracy said:
Quote:

Sgt. Pepper said:
And a 16 quart really isn't that bad. You can get several dozen done in a couple days if you stay up late.



so far i've only done 14 grain jars in one day ,but probably could do 21:shrug:




I've done 36 in 2 days. And if you're doing bags you could probably get more. I can fit at least 10 quarts of birdseed if I'm using bags. I could probably fit 15, but I'm scared the bags will swell and plug the hole that releases pressure.


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Offlineitsaconspiracy
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Re: frozen grains [Re: Sgt. Pepper]
    #19379842 - 01/06/14 10:21 PM (10 years, 24 days ago)

yea thats thats the only reason i haven't tried to do bags yet i'm afraid it might blow :onfire: or the bag blow from pressure.


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Re: frozen grains [Re: itsaconspiracy]
    #19379847 - 01/06/14 10:23 PM (10 years, 24 days ago)

Quote:

highc said:
2 all american 30quart cookers. O yea I need that much!


:gonnaneedmyglasses: whoa if you need that much post more grows:lol:




No serious grows sorry.

I might do up my current grow.


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Re: frozen grains [Re: highc]
    #19379880 - 01/06/14 10:28 PM (10 years, 24 days ago)

cool:thumbup:


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Re: frozen grains [Re: itsaconspiracy]
    #19379935 - 01/06/14 10:40 PM (10 years, 24 days ago)

Yea i got 7 golden teacher jars. 1 golden teacher bag. 5 b+ jars. 1 south american rye bag. And 1 i believe it to be a brown rice or flour bag. Never seen one like it but I went ahead and just knocked it up with south american.

Idk what IMA do with the south americans since i have just a little but will worry about that when the time arrives.

I document all my grows very well. Written anyway. I will take pictures throughout in case I decide to post. And i will do so the day I throw it allout so it will be complete upon post.


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Re: frozen grains [Re: highc]
    #19379945 - 01/06/14 10:41 PM (10 years, 24 days ago)

:waitingpatiently:


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Re: frozen grains [Re: Sgt. Pepper]
    #19379959 - 01/06/14 10:44 PM (10 years, 24 days ago)

Quote:

Sgt. Pepper said:
I could probably fit 15, but I'm scared the bags will swell and plug the hole that releases pressure.




That is why you put a heavy plate on top of your bags before PCing. I do four 5-quart bags per run in each of my AA-921s.


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Re: frozen grains [Re: cronicr]
    #19379980 - 01/06/14 10:48 PM (10 years, 24 days ago)

Quote:

cronicr said:
:waitingpatiently:




Quote:

FrankHorrigan said:
That is why you put a heavy plate on top of your bags before PCing.



,but wont that cause the bags to seal up and build up too much pressure and :booooom:?


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Re: frozen grains [Re: itsaconspiracy]
    #19379995 - 01/06/14 10:51 PM (10 years, 24 days ago)

The bags should be sealed already..


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Offlineitsaconspiracy
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Re: frozen grains [Re: highc]
    #19380008 - 01/06/14 10:54 PM (10 years, 24 days ago)

??? so how will a person put a grain inoculate in it or even inoculate it? (sorry my noob is showing:eek:)


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Re: frozen grains [Re: itsaconspiracy]
    #19380017 - 01/06/14 10:56 PM (10 years, 24 days ago)

Freezing temperatures won't kill the spores if that's what you're worried about. They're not going germinate and grow while that cold obviously but they'll still be viable when the jar warms back up.


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Re: frozen grains [Re: highc]
    #19380026 - 01/06/14 10:57 PM (10 years, 24 days ago)

I considered this, I even put the plate ontop of all 3 bags and closed the pc, but I just couldn't get the nerve to start it. It just looked so full and wrong! I had another bag that needed to be pc'd so I figured I'd do 2 bags twice instead of 3 bags then 1 bag. Maybe when I have 16 bags to pc I'll cram that extra one into the pc.


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Re: frozen grains [Re: Sgt. Pepper]
    #19380073 - 01/06/14 11:08 PM (10 years, 24 days ago)



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Yes, you can bump my old threads with a question.
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You should take a look. :hehehe:


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Re: frozen grains [Re: itsaconspiracy]
    #19380074 - 01/06/14 11:09 PM (10 years, 24 days ago)

Quote:

itsaconspiracy said:
??? so how will a person put a grain inoculate in it or even inoculate it? (sorry my noob is showing:eek:)




You can make an inoculation point. Anywhere you want really. Or you can just stick it through the bag, squirt, and cover the hole with tape as your pulling the needle out.


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Re: frozen grains [Re: highc]
    #19380131 - 01/06/14 11:20 PM (10 years, 24 days ago)

Place a tyvek sleeve inside the bag and fold it accordion-style as RR shows in his sawdust video. That's all there is to it.


--------------------

Yes, you can bump my old threads with a question.
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You should take a look. :hehehe:


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Re: frozen grains [Re: highc]
    #19380181 - 01/06/14 11:32 PM (10 years, 24 days ago)

Quote:

FrankHorrigan said:
Place a tyvek sleeve inside the bag and fold it accordion-style as RR shows in his sawdust video. That's all there is to it.




cool then can you seal it after words?

Quote:

highc said:
Quote:

itsaconspiracy said:
??? so how will a person put a grain inoculate in it or even inoculate it? (sorry my noob is showing:eek:)




You can make an inoculation point. Anywhere you want really. Or you can just stick it through the bag, squirt, and cover the hole with tape as your pulling the needle out.




what type of tape would you use to seal up the hole?


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Re: frozen grains [Re: itsaconspiracy]
    #19380189 - 01/06/14 11:35 PM (10 years, 24 days ago)

I use duct tape and write the strain and date on it. You don't want to use the micropore tape. Though I guess it wouldn't hurt. So I should say you don't HAVE to use it.


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Re: frozen grains [Re: highc]
    #19380195 - 01/06/14 11:39 PM (10 years, 24 days ago)

cool:thumbup: do you inoculate and tape up in a SAB or open air?


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Re: frozen grains [Re: itsaconspiracy]
    #19380198 - 01/06/14 11:40 PM (10 years, 24 days ago)

Inoculate the bag with a master grain jar so you do not have to puncture a hole in it.

Quote:

itsaconspiracy said:
cool:thumbup: do you inoculate and tape up in a SAB or open air?




If you have an exposed opening into your bag of sterile grains, you should always, always, always work in aseptic conditions such as a SAB.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
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Re: frozen grains [Re: itsaconspiracy]
    #19380205 - 01/06/14 11:45 PM (10 years, 24 days ago)

I use open air. I think a sab is over kill for standard inoculation. Just in my opinion though. And experience.


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Re: frozen grains [Re: FrankHorrigan]
    #19380209 - 01/06/14 11:45 PM (10 years, 24 days ago)

Quote:

FrankHorrigan said:
Inoculate the bag with a master grain jar so you do not have to puncture a hole in it.

Quote:

itsaconspiracy said:
cool:thumbup: do you inoculate and tape up in a SAB or open air?




If you have an exposed opening into your bag of sterile grains, you should always, always, always work in aseptic conditions such as a SAB.




Fair point.


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Re: frozen grains [Re: FrankHorrigan]
    #19380219 - 01/06/14 11:49 PM (10 years, 24 days ago)

Quote:

FrankHorrigan said:
Inoculate the bag with a master grain jar so you do not have to puncture a hole in it.

Quote:

itsaconspiracy said:
cool:thumbup: do you inoculate and tape up in a SAB or open air?




If you have an exposed opening into your bag of sterile grains, you should always, always, always work in aseptic conditions such as a SAB.




cool its better safe than sorry and risk a contam now sealing do you seal them or will a zip tie be enough to close it?


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Re: frozen grains [Re: itsaconspiracy]
    #19380284 - 01/07/14 12:08 AM (10 years, 24 days ago)

Seal. 2mm if sealing prior to PC.


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