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Offlineravenfeeder
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Does this look like yeasty?
    #19371542 - 01/05/14 06:45 AM (10 years, 1 month ago)

Hi All,

G2g'ed some jars two days ago from one fully colonized Costa Rican master grain jar. All three jars I g2g'ed to showed these symptoms, and I do surmise it is from the wedge dropped into the original master jar. Alas it is colonizing normally:dancer:

When it is fully colonized, will it be safe to spawn to bulk? Costa Rica as a strain seems to have no issue with this condition.

Regards,
Rf
:mushroom2:



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OfflinePussyFart
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Re: Does this look like yeasty? [Re: ravenfeeder]
    #19371576 - 01/05/14 07:06 AM (10 years, 1 month ago)

That jar is fucked.


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Offlinetripdawg420
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Re: Does this look like yeasty? [Re: PussyFart]
    #19371691 - 01/05/14 07:47 AM (10 years, 1 month ago)

is that plastic over the lid


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OfflineCliftonGK1
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Re: Does this look like yeasty? [Re: tripdawg420]
    #19371756 - 01/05/14 08:20 AM (10 years, 1 month ago)

Not sure what the contam is, but from experience, it won't end well.  I g2g'd a jar that looked a bit off according to some of the more experienced cultivators, and even though the jar colonized fine and didn't have any particular reek when I spawned it out, *and* it even colonized the bulk tub fine, the contam won out in the end and it never fruited.
Lots of wasted effort.  Save your time and work and start over.


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Offlineravenfeeder
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Re: Does this look like yeasty? [Re: CliftonGK1]
    #19371907 - 01/05/14 09:19 AM (10 years, 1 month ago)

Quote:

tripdawg420 said:
is that plastic over the lid




Yup! I wrap the seal of the lids with Seran wrap roll I get from my lab, just to make extra sure no shit may ever get in from the sides :stoned:


Quote:

CliftonGK1 said:
Not sure what the contam is, but from experience, it won't end well.  I g2g'd a jar that looked a bit off according to some of the more experienced cultivators, and even though the jar colonized fine and didn't have any particular reek when I spawned it out, *and* it even colonized the bulk tub fine, the contam won out in the end and it never fruited.
Lots of wasted effort.  Save your time and work and start over.




Kinda the answer I was looking for and fearing. Lol. Because I too reckon that if it goes on colonizing the grains happily, how can it f%*k out at bulking/fruiting? You will see the uncolonized grains seems to have fine sandlike particles on them. Apparently that is a yeast contamination.

Thanks notahacker420 for making it extra clear:stoned:


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OfflineCliftonGK1
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Re: Does this look like yeasty? [Re: ravenfeeder]
    #19372263 - 01/05/14 10:44 AM (10 years, 1 month ago)

Quote:

ravenfeeder said:
Quote:

CliftonGK1 said:
Not sure what the contam is, but from experience, it won't end well.  I g2g'd a jar that looked a bit off according to some of the more experienced cultivators, and even though the jar colonized fine and didn't have any particular reek when I spawned it out, *and* it even colonized the bulk tub fine, the contam won out in the end and it never fruited.
Lots of wasted effort.  Save your time and work and start over.




Kinda the answer I was looking for and fearing. Lol. Because I too reckon that if it goes on colonizing the grains happily, how can it f%*k out at bulking/fruiting? You will see the uncolonized grains seems to have fine sandlike particles on them. Apparently that is a yeast contamination.

Thanks notahacker420 for making it extra clear:stoned:




In my case it wasn't yeast, but something bacterial.  It affected the appearance of the mycelial growth but not the speed or strength.  The mycelium won the short-term battle for resources when it came to colonizing the grains.  However, when it was spawned out to a tub, there was enough of both myc and contam available that the contam could grab a foothold even as the mycelium was colonizing the bulk sub.  In the end, even though the entire sub colonized and consolidated, the contaminant had grown in such a concentration to keep the whole thing from fruiting.
I put it outside and let it go for a while longer to see what would happen, and over the next week the entire thing took on a mild yellowish colour and a slightly acrid odour.  Not a mycelial metabolite colouration; it was obvious that it was the contaminant growth taking things over.

As for it being a yeast contamination on yours, I used to see those in cell culture work.  The most common reason was somebody waiting on a culture step like trypsinization got bored, took their hands out of the hood, and mindlessly did something like play with their earring.  Boom, there it was; if you don't change gloves before going back in, it's all over!  (Caught that one 4 times at 3 different jobs, usually student techs.)  The one that really pissed me off was a guy I didn't want to hire but apparently he was one of the lab director's friend's kids.  He was just dirty; like same clothes 4 days in a row dirty.  Turns out, he was also a homebrewer, and we ended up with consistent yeast contam issues because this dude would work with his beer the night before and then come in with it on his clothes.  Not making any assumptions, but always always, clean clothes when doing culture work.


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Offlineravenfeeder
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Re: Does this look like yeasty? [Re: CliftonGK1]
    #19373002 - 01/05/14 01:26 PM (10 years, 1 month ago)

Ah, bacterial - yeah that's always bad. I also just threw out a tray that was cased and looking prime, then after a month plus of not fruiting I tossed it. The underside was all brown, stinky and gunky. This was because I used grains that had a few grains of wet spot. Even though it fully colonized, it never fruited. Was a valuable learning experience though!

Because I still have to see what happens, I will spawn the grains to bulk if it fully colonizes in a week or less. I'd really like to see what happens then. Even if it means to gain some more hands-on experience, and if it does fruit, well, fat bonus I suppose!:dancer:

Haha, I pity you on having to have worked with such mindless individuals :eek: I do also take all the precautions, shower, fresh clothes, still room, freshly washed out glove box, arm covers and nitrile gloves, wipe all surfaces of jars and tools and items involved with 70% ethanol and flame sterilize. It just really gets to me when I achieve these near sterile conditions and somebody barges in the room to ask if I want coffee...:rolleyes:


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Offlinetwistedty
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Re: Does this look like yeasty? [Re: ravenfeeder]
    #19373510 - 01/05/14 03:35 PM (10 years, 1 month ago)

what kind of filter are you using? and saran wrap isnt necessary


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Offlineravenfeeder
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Re: Does this look like yeasty? [Re: twistedty]
    #19374909 - 01/05/14 09:30 PM (10 years, 1 month ago)

The filtration I am using is two layers of 3M Micropore tape on my consol jar lids covering the four 1/8" holes I have drilled through them, and then I have created my own version of sfd's by arranging five 30mm 0.22um membrane filters in a circle bigger than the lid and keeping it in place by covering the top and bottom with micropore tape. This wannabe "sfd" I then can reuse and it gets placed over the micropore taped jar lid, and secured by the ring lid, just like you would with a sfd.

:likeaboss:


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OfflinecronicrFacebook
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Re: Does this look like yeasty? [Re: ravenfeeder]
    #19375002 - 01/05/14 09:54 PM (10 years, 1 month ago)

Quote:

ravenfeeder said:
This wannabe "sfd" I then can reuse and it gets placed over the micropore taped jar lid, and secured by the ring lid, just like you would with a sfd.

:likeaboss:



i don't think you can be "like a boss" after showing a contaminated jar:laugh2:


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Offlineravenfeeder
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Re: Does this look like yeasty? [Re: cronicr]
    #19375048 - 01/05/14 10:06 PM (10 years, 1 month ago)

Hahaha! I just did that for effect. :2girls1cup:


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OfflineKizzle
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Re: Does this look like yeasty? [Re: ravenfeeder]
    #19376196 - 01/06/14 08:02 AM (10 years, 1 month ago)

Not all yeasts will prevent colonization or prevent fruiting. I have no clue though if the jar did colonize if it some yeasts could contaminate the bulk substrate. If it did I'm guessing it's something you'd smell but not see.


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Offlineravenfeeder
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Re: Does this look like yeasty? [Re: Kizzle]
    #19377162 - 01/06/14 12:54 PM (10 years, 1 month ago)

Quote:

Kizzle said:
Not all yeasts will prevent colonization or prevent fruiting. I have no clue though if the jar did colonize if it some yeasts could contaminate the bulk substrate. If it did I'm guessing it's something you'd smell but not see.




So I am gathering that I will let the jars fully colonize, and then do the smell-test after loosening up the grains, and then if it smells like mushies I go ahead and spawn to bulk?:cool:


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InvisibleFrankHorrigan
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Re: Does this look like yeasty? [Re: ravenfeeder]
    #19377179 - 01/06/14 12:58 PM (10 years, 1 month ago)

Quote:

ravenfeeder said:





That is indeed yeast.

It is prolific and awful. Sterilize those jars before dumping the contents.

Don't use it for spawn.


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Offlineravenfeeder
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Re: Does this look like yeasty? [Re: FrankHorrigan]
    #19377240 - 01/06/14 01:27 PM (10 years, 1 month ago)

Quote:

FrankHorrigan said:
Quote:

ravenfeeder said:





That is indeed yeast.

It is prolific and awful. Sterilize those jars before dumping the contents.

Don't use it for spawn.





Ah Frank! Glad to have your attention - I just wanna say kudo's to you man - I applied your tek on spawning to bulk 8 days ago. McKennaii 750ml's of grains with 1500ml's of pasteurized bulk substrate. Did 2 trays. Both of the same isolate. Same volumes of substrate and spawn, but only one's grains I soaked for 15 minutes, and that tray was finished colonizing on day 6. Other one today on day 8. Now for the consolidation wait :cheers:

Thanks a lot for awesome teks, and I will dump those shit jars asap:pirate:


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OfflineKizzle
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Re: Does this look like yeasty? [Re: ravenfeeder]
    #19378696 - 01/06/14 06:47 PM (10 years, 1 month ago)

Just want to point out there are several yeasts that are a natural part of your skin flora and that's the most likely source of contamination. It's not something you can simply eradicate. It's why we always have to wear gloves.


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