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OfflineBassbassik
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Registered: 12/03/13
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Last seen: 9 years, 11 months
Weird looking mycelium on colonized tray, need your opinions.
    #19377134 - 01/06/14 12:30 PM (10 years, 25 days ago)

Small WBS to coir tray that colonized normally. It was placed in the FC on the 1st. Temps are staying between 70/75, FEA is good and it's getting 12/12 on off.

For this round the spawn was a 90% fail rate, so I'm worried about it reaching 100%.

There are some spots of the mycelium that are yellowing but it's not myc-piss puddles, just light yellow tinted areas. A search for yellow tinted mycelium turns up nothing but threads on myc-piss and obvious contamination.

There are no obvious contams (to my eyes) on the sub. It does look wispy/thin. The lighting in the pics make it seem like there is green on that last one, but there isn't.

I've searched and searched on here but can't find anything to compare to.

If this one fails too, I'm lost. Please tell me I'm just a rookie and that this is normal mycelium :confused:







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OfflineBassbassik
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Re: Weird looking mycelium on colonized tray, need your opinions. [Re: Bassbassik]
    #19380750 - 01/07/14 02:57 AM (10 years, 24 days ago)

I finally found a nice thread for comparison on what it should look like so I'll paste it here for anyone else who has a similar question to mine:
http://www.shroomery.org/forums/showflat.php/Number/17231150/fpart/1/vc/1

Turns out the sub is fine.


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OfflineMAIA
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Re: Weird looking mycelium on colonized tray, need your opinions. [Re: Bassbassik]
    #19384261 - 01/07/14 06:33 PM (10 years, 23 days ago)

Give it a couple of days and then you'll know. My guess is high Rh causing that type of growth.

Btw, did you use a top layer ?


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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OfflineBassbassik
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Re: Weird looking mycelium on colonized tray, need your opinions. [Re: MAIA]
    #19389330 - 01/08/14 05:06 PM (10 years, 22 days ago)

It's just 1/3 wbs to coir with an even top layer of more coir. I didn't case, just let it consolidate and stuck it in the FC.

The first pins showed up today, so this round isn't a complete loss.

FC has two 50gal tank pumps fed through bubble wands in a big bottle of water, I'm not sure if the humidity's high enough. This method did work beautifully last time with Cambodians, though. :mushroom2: 

My analog hygrometer is reading 80% but the digital is at 99%. I might calibrate the analog tonight to make sure it's reading correctly.


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OfflineMAIA
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Re: Weird looking mycelium on colonized tray, need your opinions. [Re: Bassbassik]
    #19389473 - 01/08/14 05:24 PM (10 years, 22 days ago)

Quote:

It's just 1/3 wbs to coir with an even top layer of more coir. I didn't case, just let it consolidate and stuck it in the FC.




But you did apply a top layer of coir. The thing is that top layer shouldn't be colonized. At least, not that much so it can play its role. If you keep missing some pinning windows this is what happens, it get's colonized. Then it begs the question: Why did you apply a coir layer on top ?

Quote:

The first pins showed up today, so this round isn't a complete loss.




That's ok but you do understand now why you're getting a weak flush.

Quote:

My analog hygrometer is reading 80% but the digital is at 99%. I might calibrate the analog tonight to make sure it's reading correctly.




And what's the optimal Rh in this case ? I really don't know ... you have an hybrid between a failed casing and a big ass cake. Casing need less Rh than cakes and in this case I would treat it like a casing. You can probably get a stronger second flush but I really advise you to apply a top layer. Sterilize some verm and apply after you pick that first flush.


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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OfflineBassbassik
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Re: Weird looking mycelium on colonized tray, need your opinions. [Re: MAIA]
    #19390229 - 01/08/14 07:48 PM (10 years, 22 days ago)

That's the thing, I didn't case it at all. I applied a layer of coir just so that there wouldn't be any exposed grains, covered it back up and let it colonize.
Maybe I'm confused, but I thought that without a casing I should wait until it's 100% colonized and then introduce it to fruiting conditions.
I know that with a casing, you wait until the mycelium is just under the very top of the surface and then introduce it to the FC. I didn't think the fruiting window was the same without an actual casing.

If I case it with verm before the 2nd flush will it damage anything? There are plenty of knots that I don't want kill.


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OfflineMAIA
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Re: Weird looking mycelium on colonized tray, need your opinions. [Re: Bassbassik]
    #19390552 - 01/08/14 08:48 PM (10 years, 22 days ago)

Casing is the use of a protective and non nutritive layer. In your case, on top. Therefore, and technically speaking, if you did apply this layer of coir, then it's a casing and you should follow casing procedures.

Scrolldown for casing:
http://www.shroomery.org/glossary.php

The casing layer should not be colonized 100% . IMHO, more than 80%-90% can be considered as overlay.

More info about this:
http://www.shroomery.org/5231/When-should-I-put-the-casing-into-the-fruiting-chamber

Quote:

I didn't think the fruiting window was the same without an actual casing.




The pinning window must happen asap as it enters the FC. When you let the top layer colonize too much, that's what is called "overlay" and trying to make it pin becomes harder.

Quote:

If I case it with verm before the 2nd flush will it damage anything? There are plenty of knots that I don't want kill.




Forget about the knots, they won't be "killed". Just let it flush now, collect all fruitbodies and apply the casing layer. Up the temps 5 degrees Celsius, stop air exchanges and shut the lights for a week or less (follow the ideas in the second link I posted) . Then restore previous temp and adjust all remaining pinning params.

How do you plan sterilizing the verm btw ?


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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OfflineBassbassik
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Re: Weird looking mycelium on colonized tray, need your opinions. [Re: MAIA]
    #19392263 - 01/09/14 05:26 AM (10 years, 22 days ago)

I know that much about casing, I didn't think that covering the mixed spawn with a light layer of coir was the same thing since it does have nutrients and it colonizes along with the substrate. I'll definitely adjust for the next round.

Oh well, the pinset is coming in pretty good and even, all things considered. I'll have to sterilize the verm in the oven since I lent the pc out.

Thanks for the help and links MAIA :thumbup:


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OfflineMAIA
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Re: Weird looking mycelium on colonized tray, need your opinions. [Re: Bassbassik]
    #19393606 - 01/09/14 12:49 PM (10 years, 22 days ago)

You're welcome !

In regards to sterilizing verm, you can do it in the microwave. Just prepare the verm if you were PCing it. I usually soak the verm in a h2o:h2o2 solution with a 10:1 ratio. Let it rest for an hour or so, then drain it and place it inside a tupperware, or one of those microwave ready plastic bags. Nuke it for 5 minutes on max, let it cool down and you're ready to go. Btw, the bigger the amount of verm, the more you need to nuke it.

Good luck !


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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