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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Yuri's Agar Work
#19354229 - 01/01/14 10:33 AM (10 years, 1 month ago) |
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So this is gonna be where i'll post my agar attempts both positives attempts, and failures. so i can learn from my mistakes, keep all the good info you guys direct me to until im a Agar PRO!
so to start with I'm gonna go with a PE6 print i received not to long ago. my plan is to swipe a spot on the print then transfer to agar. my agar will be a recipe of MYA = Malt-yeast Extract
- H2O 500 mL
- Agar 10g
- Malt 10g
- Yeast 1g
dry ingredients are being mixed now. then added to boiling water for 1 min. then mixed, and poured to agar round dishes
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
Edited by Yuri.Pono (01/01/14 11:35 AM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19354309 - 01/01/14 11:09 AM (10 years, 1 month ago) |
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 i'm proud of you
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: cronicr]
#19354491 - 01/01/14 12:07 PM (10 years, 1 month ago) |
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so i just done my mix and letting the agar set up in the rounds to cool. got to drill out my holes while waiting then ill PC for 45 min @ 15 psi.
now from what im reading im letting them setup after my pour will help with condensation? and how long after they cool do i wait to PC?
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
Edited by Yuri.Pono (01/01/14 12:08 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19354503 - 01/01/14 12:11 PM (10 years, 1 month ago) |
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after i pour mine i wait till it gets back to room temp before putting the lid on and pc'ing
 thts right after i poured and you can see hte little condensation there, i wait for tht to go away and close the lid for a few minutes to see if any more builds up and repeat till i know it's at room temp
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: cronicr]
#19354602 - 01/01/14 12:48 PM (10 years, 1 month ago) |
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thanks will do
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Yuri's Agar Work [Re: cronicr]
#19354637 - 01/01/14 01:00 PM (10 years, 1 month ago) |
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Quote:
cronicr said: after i pour mine i wait till it gets back to room temp before putting the lid on and pc'ing
 thts right after i poured and you can see hte little condensation there, i wait for tht to go away and close the lid for a few minutes to see if any more builds up and repeat till i know it's at room temp
I wish I knew about this a while ago my pp5s are fogy ass hell.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Yuri's Agar Work [Re: MudaFuka]
#19354639 - 01/01/14 01:01 PM (10 years, 1 month ago) |
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 also helps to leave them in the pc to cool
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Yuri's Agar Work [Re: cronicr]
#19354697 - 01/01/14 01:16 PM (10 years, 1 month ago) |
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thanx for the tip. hopefully if I read enough of these threads ill actually have some success with agar. Iv had so many bacterial contams im starting to lose my fucking minde.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Yuri's Agar Work [Re: MudaFuka]
#19354710 - 01/01/14 01:19 PM (10 years, 1 month ago) |
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i had the same problems for awhile, just remember the more you can keep the temps the same on the outside as in the less condensation you will have

 so even if you take it out of the pc to quick you will end up with it
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: cronicr]
#19354799 - 01/01/14 01:48 PM (10 years, 1 month ago) |
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so far what im working with.

got a dinner date with the wife's family so im gonna just let them sit until i get back then PC.
for transfering my spores im gonna take a Q-Tip and cut 1 end off, and then take a bamboo stick and tape the 2 together. wrap in foil and PC with the agar. let it all cool over night in the PC then get to work in the morning.
do i need sterile water? or can i just dab the swab on the print and streak the agar?
just got to thinking of what i can do after i cant use these anymore. they'd make great psilly jello shot for next halloween.
Edited by Yuri.Pono (01/01/14 01:54 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19354820 - 01/01/14 01:53 PM (10 years, 1 month ago) |
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you can just dab it on the print
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19354824 - 01/01/14 01:54 PM (10 years, 1 month ago) |
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oh and Quote:
Yuri.Pono said: so far what im working with.

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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: cronicr]
#19354836 - 01/01/14 01:57 PM (10 years, 1 month ago) |
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cool so i'll streak 2 rounds clean them both up. then transfer to grain jars. g2g then spawn to bulk. find me some nice clone to put to agar and repeat to fine the strongest isolate.
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19362483 - 01/03/14 08:56 AM (10 years, 1 month ago) |
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cooked agar last night. was hoping to not have condensation on the walls as i did let it cool to room temp over night both after pour and after cooking.
on another note i'm gonna work on my PE6, and clone Escondido.


-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
Edited by Yuri.Pono (01/03/14 09:44 AM)
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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19362611 - 01/03/14 09:47 AM (10 years, 1 month ago) |
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hope if i stick these on their side that the condensation will pool up like on my glass one and ill pour out b4 working. as they weren't terribly bad as the glass ones was.
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19363104 - 01/03/14 12:03 PM (10 years, 1 month ago) |
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conedensation is not the end of the world itf you leave it for a couple days it will get absorbed back into the agar, sometimes it's just impossible to avoid and i think it has something to do with how fast we let the pressure drop when we shut the pc off, but like i said it's no biggies anyway
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: cronicr]
#19374745 - 01/05/14 08:48 PM (10 years, 1 month ago) |
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question. i havent got to my agar work but plan to in the am Thursday if not b4. work is kicking myass atm. but i do pick 3 fruit for cloning all 3 come from cluster and are meaty. i double bagged them and tossed in frig until then will that be ok?
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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magickspore



Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 8 months, 29 days
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19374753 - 01/05/14 08:54 PM (10 years, 1 month ago) |
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If they are in plastic they will go bad fast. I believe a paper bag would be best.
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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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shit that sucks no brown bags here. how about tyvek bag?
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19374814 - 01/05/14 09:11 PM (10 years, 1 month ago) |
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worst comes to worst the agar will go to my PE6 print
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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