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Offlineeelrd72
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guessing lipstick
    #19374273 - 01/05/14 06:41 PM (10 years, 25 days ago)

here is some contaminates..i am the master at growing contaminates



im guessing its lipstick. I did these on agar, transfered to rye grains following RR video but they always seem too wet..even tho I shake 30 to 45 minutes..tissue test. I didnt know to add the filter to the intake of blower when I did grain to grain. One of these days Im going to learn how to do this correctly.


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InvisibleGanzig
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Re: guessing lipstick [Re: eelrd72]
    #19374326 - 01/05/14 06:55 PM (10 years, 25 days ago)

How are you sterilizing your grain jars?

Tell me what equipment and your pc time.


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Offlinemudbutt
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Re: guessing lipstick [Re: Ganzig]
    #19374339 - 01/05/14 06:59 PM (10 years, 25 days ago)

Too wet is always bad!!    I dont boil my seed ....I just soak for 12-24 hours rinse drain and then pour my seed on a towel to pat it dry.....I seem to have less contamination problems keeping my seed... not so wet


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Offlineeelrd72
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Re: guessing lipstick [Re: mudbutt]
    #19374358 - 01/05/14 07:06 PM (10 years, 25 days ago)

after 24 soak...i boil for 10 minutes...then i shake for 30 to 45 minutes..might even let them sit for another 15 minutes. I put foil over the tops and pressure cook for 90 minutes..at 15psi. I use table spoon of gypsum with 7 to 9 jars.


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InvisibleGanzig
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Re: guessing lipstick [Re: eelrd72]
    #19374381 - 01/05/14 07:11 PM (10 years, 25 days ago)

Any chance your flow hood is not working correctly?

What do you mean by "shake for 30 to 45 mins"?


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Offlinemudbutt
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Re: guessing lipstick [Re: Ganzig]
    #19374396 - 01/05/14 07:14 PM (10 years, 25 days ago)

Quote:

Ganzig said:[/

What do you mean by "shake for 30 to 45 mins"?



:whathesaid:


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Offlineeelrd72
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Re: guessing lipstick [Re: Ganzig]
    #19374455 - 01/05/14 07:30 PM (10 years, 25 days ago)

yeah pretty sure it was my flowhood on these...I didnt have a filter in front of my blower intake. I shake like the RR video. To steam dry.


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InvisibleGanzig
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Re: guessing lipstick [Re: eelrd72]
    #19374500 - 01/05/14 07:41 PM (10 years, 25 days ago)

Quote:

eelrd72 said:
yeah pretty sure it was my flowhood on these...I didnt have a filter in front of my blower intake. I shake like the RR video. To steam dry.




The pre-filter for the intake is not necessary for the flow hood to work.

It only helps with longevity of the main filter.


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OfflineKizzle
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Re: guessing lipstick [Re: Ganzig]
    #19375934 - 01/06/14 05:59 AM (10 years, 25 days ago)

The red stuff is metabolites produced by that green mold. My guess, considering how consistent the contamination is in all those jars, is you accidentally inoculated them with something other than mushroom mycelium or at least a contaminated culture or g2g master.

You can't rule out the possibility of the flowhood blowing mold spores into them though, possibly even something that came between the filter and the jars during the inoculation.

However you can rule out sterilization and grain preparation.


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Offlinecli_hlt
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Re: guessing lipstick [Re: mudbutt]
    #19380650 - 01/07/14 02:05 AM (10 years, 24 days ago)

Agreed.

I also noticed that when I get no condensation and exploded kernels, I get almost no contaminants even with open-air inoculation with agar pieces (I'm quick though :wink: ).

Same with agar: if there's a lot of condensation, then I get quite a few contaminated plates. Few condensation -> almost no contamination in a simple SAB.


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OfflineShaulyn
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Registered: 01/16/19
Posts: 6
Last seen: 4 years, 11 months
Re: guessing lipstick [Re: cli_hlt]
    #25746926 - 01/16/19 06:12 AM (5 years, 14 days ago)

tell me what is it? povilas before the last wave (before this redness was not observed), took the harvest and was about to throw out the mycelium, and saw this redness


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