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InvisibleTheLoopIs

Registered: 05/29/13
Posts: 330
contam precaution after inoculation?
    #19372962 - 01/05/14 01:21 PM (10 years, 25 days ago)

Never understood this...When I inoculate my jars am supposed to take some contam precaution like cover it back up in tin foil for a few days or microtape?Or just let it be and my verm layer will take care of it?

If so what the hell is the point of being so sterile with the environment around when inoculating?


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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
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Re: contam precaution after inoculation? [Re: TheLoopIs]
    #19372989 - 01/05/14 01:24 PM (10 years, 25 days ago)

Quote:

TheLoopIs said:
Never understood this...When I inoculate my jars am supposed to take some contam precaution like cover it back up in tin foil for a few days or microtape?Or just let it be and my verm layer will take care of it?




Leave the foil off, having it on can make your dry verm layer damp, which defeats the point of a dry verm layer

Quote:

TheLoopIs said:
If so what the hell is the point of being so sterile with the environment around when inoculating?




Inoculation is the point that your substrate or spawn is most susceptible to contam. You have uncolonized sterile media which can easily be taken over by competing organisms.


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: contam precaution after inoculation? [Re: TheLoopIs]
    #19373034 - 01/05/14 01:32 PM (10 years, 25 days ago)

Quote:

TheLoopIs said:
just let it be and my verm layer will take care of it?

If so what the hell is the point of being so sterile with the environment around when inoculating?





Yep surprisingly the dry verm layer is a great filter.  Your best bet is to not shake, touch or overly handle your jars.  IF a contamination finds its way to the verm layer and it gets shook down to the nutritious substrate it will destroy the substrate.

The point of being sterile is to not push contamination down into the nutritious substrate.  As absurd as it may seem, that dry layer of rocks has been proven time and time again as effective.


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OfflineKnownPown
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Registered: 10/27/12
Posts: 219
Loc: Washington State
Last seen: 6 years, 6 months
Re: contam precaution after inoculation? [Re: HypnotoadCroaked]
    #19373379 - 01/05/14 02:56 PM (10 years, 25 days ago)

Another reason we're so sterile when we inoculate is because after we flame sterilize the needle of the syringe, the time it takes to cool off the syringe by squirting a small drop (so your spores dont die) and then bring it to your hole for inoculation, whatever in the air has a chance to land on the needle and be pushed into your highly nutritious substrate.

Give it the conditions your mushrooms would like to grow in like 70-80 degree temps, and any contams will also grow. Gotta keep it clean from the beginning;)

But even then the spores in your syringe aren't going to ever be 100% clean, which is why people move on to agar =)


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Offlinethoraxx
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Registered: 12/27/13
Posts: 580
Loc: Bavaria
Last seen: 4 years, 10 months
Re: contam precaution after inoculation? [Re: KnownPown]
    #19373737 - 01/05/14 04:28 PM (10 years, 25 days ago)

Quote:

KnownPown said:the time it takes to cool off the syringe by squirting a small drop (so your spores dont die) and then bring it to your hole for inoculation, whatever in the air has a chance to land on the needle and be pushed into your highly nutritious substrate.



I always felt it makes much more sense to stick the needle in while its still red hot
I guess that depends on what kinda inoculation port you're using


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OfflineKnownPown
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Registered: 10/27/12
Posts: 219
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Last seen: 6 years, 6 months
Re: contam precaution after inoculation? [Re: thoraxx]
    #19374497 - 01/05/14 07:41 PM (10 years, 25 days ago)

Well for pf cakes you should just stick to the dry verm layer, it works out relly well if you do everything else right. But I do it just before I put it in, I have shot it in too hot before and gotten nothing at the same time though /:


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 14 hours
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Re: contam precaution after inoculation? [Re: KnownPown]
    #19374516 - 01/05/14 07:44 PM (10 years, 25 days ago)

Quote:

KnownPown said:
I have shot it in too hot before and gotten nothing at the same time though /:



The first drop or 2 will cool the needle and the rest will flow contam free.

There is no way the heat from the needle caused you to fail.


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OfflineKnownPown
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Registered: 10/27/12
Posts: 219
Loc: Washington State
Last seen: 6 years, 6 months
Re: contam precaution after inoculation? [Re: PussyFart]
    #19374621 - 01/05/14 08:12 PM (10 years, 25 days ago)

Hmm maybe not, maybe I just got no spores in that part of the syringe or they just never germinated even after a shake and whatnot. Those mysterious jars...


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