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supernewfie
some fella


Registered: 09/24/13
Posts: 1,103
Loc: Canada
Last seen: 5 years, 5 months
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PDA petri dish pins
#19372834 - 01/05/14 12:57 PM (10 years, 1 month ago) |
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i im not exactly sure what I can do with them, I didnt think pda was nutritious enough to support fruiting, and they did go through a cold spurt recently. Anyways thought id show you guys..
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 2 months, 4 days
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basically means that that is a strong fruiting variety.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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toss one on another dish
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: PDA petri dish pins [Re: cronicr]
#19372913 - 01/05/14 01:12 PM (10 years, 1 month ago) |
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Quote:
cronicr said: toss one on another dish

Some of my best clones started out this way
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 2 months, 4 days
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Quote:
Pastywhyte said:
Quote:
cronicr said: toss one on another dish

Some of my best clones started out this way 
yeah I much prefer taking isolates from fruits. So much easier than starting with spores..
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supernewfie
some fella


Registered: 09/24/13
Posts: 1,103
Loc: Canada
Last seen: 5 years, 5 months
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No pda
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Just make some, you gotta a day or so to get it together.
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supernewfie
some fella


Registered: 09/24/13
Posts: 1,103
Loc: Canada
Last seen: 5 years, 5 months
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What if I transfer it to grain? Or grass seed

This is a cake I made with vermiculite, used in the same ratio as brf cakes but instead of using brf, I used an organic pancake mix.
Edited by supernewfie (01/05/14 02:59 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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better off to agar then to grain....not sure y in your case asit should be pretty clean but i still say agar lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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supernewfie
some fella


Registered: 09/24/13
Posts: 1,103
Loc: Canada
Last seen: 5 years, 5 months
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Re: PDA petri dish pins [Re: cronicr]
#19373426 - 01/05/14 03:11 PM (10 years, 1 month ago) |
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The problem is I do have new dishes but no agar.
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Psilosopherr
A psilly goose



Registered: 02/15/12
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Last seen: 2 months, 4 days
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Quote:
supernewfie said: The problem is I do have new dishes but no agar.
have you heard of just putting small amounts of WBS or BRF in the bottom of a jar and using that as a ghetto agar?
Does anyone know how well that works btw?
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MarcusFreeman



Registered: 09/16/13
Posts: 376
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-------------------- "The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK As one ends, another begins.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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 it works, not good for isolating but cleaning and cloning seem to work great
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 2 months, 4 days
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Re: PDA petri dish pins [Re: cronicr]
#19373660 - 01/05/14 04:10 PM (10 years, 1 month ago) |
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Quote:
cronicr said:


 it works, not good for isolating but cleaning and cloning seem to work great
ha, didn't realize you'd done a write-up on that specifically.
I assume agar is better if you have it though. I always have plenty
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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yes agar is far better but when your in need brf or any grain flour/water mix will work it's just really high in nutrition
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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MarcusFreeman



Registered: 09/16/13
Posts: 376
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Re: PDA petri dish pins [Re: cronicr]
#19373698 - 01/05/14 04:19 PM (10 years, 1 month ago) |
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Quote:
cronicr said: yes agar is far better but when your in need brf or any grain flour/water mix will work it's just really high in nutrition
Good stuff!
-------------------- "The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK As one ends, another begins.
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supernewfie
some fella


Registered: 09/24/13
Posts: 1,103
Loc: Canada
Last seen: 5 years, 5 months
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This is a organic cracker contains many different grains and seeds.il take a picture of the box later maybe and its complete ingredients. I made 3 petris with this stuff because it seems to like it, I transferred 1 of the mushrooms into one of the petris and I cut mycelium out of some of my other petris and put into my other 2 hydrated cracker petris. I kind of am expecting a contam from them no doubt, I had to remove over half of the cracker from a petri dish because there was a sign of mold but it seems so far its doing alright again the mold hasnt come back now hopefully the mycelium can take over the little bit of cracker thats left before mold can get a hold on it

Here more pins are forming, most of my dishes (about 4) have these knots forming
Edited by supernewfie (01/06/14 11:36 AM)
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supernewfie
some fella


Registered: 09/24/13
Posts: 1,103
Loc: Canada
Last seen: 5 years, 5 months
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This is half of a grain jar I inoculated with agar about a week or so ago, I split it today because the bottom half of the jar seemed like it stalled, so the top half with most of the thicker mycelium is here, separated it in hopes the uncolonized part dont contam this part, and I still have the bottom half in a jar to see if it will colonize the rest of the way.
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MarcusFreeman



Registered: 09/16/13
Posts: 376
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Not exactly the conventional tek follower, ey? 
It's cool though. Gotta experiment some how. If I were you, I'd make up some of the brf flour cloning substrate that cronicr wrote up about. Should be pretty easy and much less likely to contaminate.
-------------------- "The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK As one ends, another begins.
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CliftonGK1
Sasquatch



Registered: 03/27/13
Posts: 327
Loc: A place
Last seen: 25 days, 6 hours
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Quote:
supernewfie said:
i im not exactly sure what I can do with them, I didnt think pda was nutritious enough to support fruiting, and they did go through a cold spurt recently. Anyways thought id show you guys..
Scalpel those guys and transfer to a new plate. Here's one I transferred only 24 hours ago, already threading out to the new agar. This one was started on PDYA and transferred to MEAY because that's what I had handy. But I've had plates pin on both agars.
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