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Offlinesupernewfie
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PDA petri dish pins
    #19372834 - 01/05/14 12:57 PM (10 years, 25 days ago)

 

i im not exactly sure what I can do with them, I didnt think pda was nutritious enough to support fruiting, and they did go through a cold spurt recently. Anyways thought id show you guys..


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OfflinePsilosopherr
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Re: PDA petri dish pins [Re: supernewfie]
    #19372842 - 01/05/14 12:59 PM (10 years, 25 days ago)

basically means that that is a strong fruiting variety.


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Re: PDA petri dish pins [Re: Psilosopherr]
    #19372850 - 01/05/14 01:00 PM (10 years, 25 days ago)

toss one on another dish


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Re: PDA petri dish pins [Re: cronicr]
    #19372913 - 01/05/14 01:12 PM (10 years, 25 days ago)

Quote:

cronicr said:
toss one on another dish



:whathesaid:

Some of my best clones started out this way :thumbup:


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OfflinePsilosopherr
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Re: PDA petri dish pins [Re: Pastywhyte]
    #19372924 - 01/05/14 01:13 PM (10 years, 25 days ago)

Quote:

Pastywhyte said:
Quote:

cronicr said:
toss one on another dish



:whathesaid:

Some of my best clones started out this way :thumbup:



yeah I much prefer taking isolates from fruits. So much easier than starting with spores..


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Re: PDA petri dish pins [Re: Pastywhyte]
    #19372929 - 01/05/14 01:14 PM (10 years, 25 days ago)

No pda :frown:


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Re: PDA petri dish pins [Re: supernewfie]
    #19372934 - 01/05/14 01:16 PM (10 years, 25 days ago)

Just make some, you gotta a day or so to get it together.


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Re: PDA petri dish pins [Re: Pastywhyte]
    #19373305 - 01/05/14 02:40 PM (10 years, 25 days ago)

What if I transfer it to grain? Or grass seed


 






This is a cake I made with vermiculite, used in the same ratio as brf cakes but instead of using brf, I used an organic pancake mix.





Edited by supernewfie (01/05/14 02:59 PM)


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Re: PDA petri dish pins [Re: supernewfie]
    #19373309 - 01/05/14 02:41 PM (10 years, 25 days ago)

better off to agar then to grain....not sure y in your case asit should be pretty clean but i still say agar lol


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Re: PDA petri dish pins [Re: cronicr]
    #19373426 - 01/05/14 03:11 PM (10 years, 25 days ago)

The problem is I do have new dishes but no agar.


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OfflinePsilosopherr
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Re: PDA petri dish pins [Re: supernewfie]
    #19373431 - 01/05/14 03:11 PM (10 years, 25 days ago)

Quote:

supernewfie said:
The problem is I do have new dishes but no agar.



have you heard of just putting small amounts of WBS or BRF in the bottom of a jar and using that as a ghetto agar?

Does anyone know how well that works btw?


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Re: PDA petri dish pins [Re: Psilosopherr]
    #19373503 - 01/05/14 03:32 PM (10 years, 25 days ago)

https://www.shroomery.org/forums/showflat.php/Number/19296230

He made an "agar" out of brf and water..... check it out


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

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Re: PDA petri dish pins [Re: MarcusFreeman]
    #19373521 - 01/05/14 03:39 PM (10 years, 25 days ago)






it works, not good for isolating but cleaning and cloning seem to work great


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Re: PDA petri dish pins [Re: cronicr]
    #19373660 - 01/05/14 04:10 PM (10 years, 25 days ago)

Quote:

cronicr said:





it works, not good for isolating but cleaning and cloning seem to work great



ha, didn't realize you'd done a write-up on that specifically.

I assume agar is better if you have it though. I always have plenty


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Re: PDA petri dish pins [Re: Psilosopherr]
    #19373667 - 01/05/14 04:13 PM (10 years, 25 days ago)

yes agar is far better but when your in need brf or any grain flour/water mix will work it's just really high in nutrition


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Re: PDA petri dish pins [Re: cronicr]
    #19373698 - 01/05/14 04:19 PM (10 years, 25 days ago)

Quote:

cronicr said:
yes agar is far better but when your in need brf or any grain flour/water mix will work it's just really high in nutrition



:thumbup:  Good stuff!


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

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Re: PDA petri dish pins [Re: supernewfie]
    #19376942 - 01/06/14 11:35 AM (10 years, 25 days ago)





This is a organic cracker contains many different grains and seeds.il take a picture of the box later maybe and its complete ingredients. I made 3 petris with this stuff because it seems to like it, I transferred 1 of the mushrooms into one of the petris and I cut mycelium out of some of my other petris and put into my other 2 hydrated cracker petris. I kind of am expecting a contam from them no doubt, I had to remove over half of the cracker from a petri dish because there was a sign of mold but it seems so far its doing alright again the mold hasnt come back now hopefully the mycelium can take over the little bit of cracker thats left before mold can get a hold on it



Here more pins are forming, most of my dishes (about 4) have these knots forming



Edited by supernewfie (01/06/14 11:36 AM)


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Re: PDA petri dish pins [Re: supernewfie]
    #19377623 - 01/06/14 03:03 PM (10 years, 24 days ago)

This is half of a grain jar I inoculated with agar about a week or so ago, I split it today because the bottom half of the jar seemed like it stalled, so the top half with most of the thicker mycelium is here, separated it in hopes the uncolonized part dont contam this part, and I still have the bottom half in a jar to see if it will colonize the rest of the way.



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Re: PDA petri dish pins [Re: supernewfie]
    #19377687 - 01/06/14 03:14 PM (10 years, 24 days ago)

Not exactly the conventional tek follower, ey? :smile:

It's cool though. Gotta experiment some how. If I were you, I'd make up some of the brf flour cloning substrate that cronicr wrote up about. Should be pretty easy and much less likely to contaminate.


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Re: PDA petri dish pins [Re: supernewfie]
    #19377995 - 01/06/14 04:24 PM (10 years, 24 days ago)

Quote:

supernewfie said:
 

i im not exactly sure what I can do with them, I didnt think pda was nutritious enough to support fruiting, and they did go through a cold spurt recently. Anyways thought id show you guys..




Scalpel those guys and transfer to a new plate.  Here's one I transferred only 24 hours ago, already threading out to the new agar.
This one was started on PDYA and transferred to MEAY because that's what I had handy.  But I've had plates pin on both agars.


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Re: PDA petri dish pins [Re: CliftonGK1]
    #19378807 - 01/06/14 07:07 PM (10 years, 24 days ago)

I have no agar and I would probably have to order it online

I may try the brf agar-type experiment


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Re: PDA petri dish pins [Re: supernewfie]
    #19378950 - 01/06/14 07:35 PM (10 years, 24 days ago)

:thumbup:


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Re: PDA petri dish pins [Re: MarcusFreeman]
    #19379504 - 01/06/14 09:16 PM (10 years, 24 days ago)

Quote:

MarcusFreeman said:
https://www.shroomery.org/forums/showflat.php/Number/19296230

He made an "agar" out of brf and water..... check it out




funny was just reading this earlyer


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Re: PDA petri dish pins [Re: supernewfie]
    #19381200 - 01/07/14 07:33 AM (10 years, 24 days ago)

Its growing so quickly I think im going to leave this one il transfer pins from another dish to clone I just want to watch the progress of this one. My first grow ever could you blame me lol, it just looks soo fucking awesome :p



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Re: PDA petri dish pins [Re: supernewfie]
    #19381546 - 01/07/14 09:43 AM (10 years, 24 days ago)

:o I put it in yesterday, its fuzzing up and looks like it will take to the cookie!!



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Re: PDA petri dish pins [Re: supernewfie]
    #19381583 - 01/07/14 09:53 AM (10 years, 24 days ago)

So... why is that grain in the dish?

The agar should have all the nutrient needed for growth and cloning. Adding grain and frequently opening the Petris will increase the chance of contams getting on the grain and agar.


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



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Re: PDA petri dish pins [Re: MarcusFreeman]
    #19381641 - 01/07/14 10:12 AM (10 years, 24 days ago)

I boiled the grain and treated it like agar in a way and transferred it to the dish and when it cooled I transferred the mushroom onto it and taped it down on 2 sides... I havent got agar, I will try the brf agar with another pin forming on a different petri...


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Re: PDA petri dish pins [Re: MarcusFreeman]
    #19381664 - 01/07/14 10:18 AM (10 years, 24 days ago)

Ohh the rye seed, I spread some on hoping the mycelium would take to it to give it a bit of extra nutrition.. I figured it might support the mushrooms growth? Maybe not though, and I have the cover completely off now, inside a microwaveable container with perlite and water underneath it.


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Re: PDA petri dish pins [Re: supernewfie]
    #19381761 - 01/07/14 10:42 AM (10 years, 24 days ago)

Well, that grain probably won't do a whole lot to support the mushy growth at this point. The mycelium would have already needed to break it down and have it ready to be used as energy for growth. Since it already has most of its energy focused on  reproduction, it likely won't benefit from added nutrient.

You say this is your first grow. Have you not done a PF cake yet?


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



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Re: PDA petri dish pins [Re: MarcusFreeman]
    #19381787 - 01/07/14 10:48 AM (10 years, 24 days ago)

Ive done a cake and it ruined way to wet.. verm and organic pancake mix it started awesome but I shot way too much spore water in. Had to dump it yesterday smelled aweful


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Re: PDA petri dish pins [Re: supernewfie]
    #19381892 - 01/07/14 11:13 AM (10 years, 24 days ago)

Quote:

supernewfie said:
I boiled the grain and treated it like agar in a way and transferred it to the dish and when it cooled I transferred the mushroom onto it and taped it down on 2 sides.



I will be shocked if that does not contam.

Quote:

supernewfie said:
Ive done a cake and it ruined way to wet.. verm and organic pancake mix it started awesome but I shot way too much spore water in. Had to dump it yesterday smelled aweful



Seriously,  pancake mix? :wow:

Quote:

supernewfie said:
Ohh the rye seed, I spread some on hoping the mycelium would take to it to give it a bit of extra nutrition.. I figured it might support the mushrooms growth? Maybe not though, and I have the cover completely off now, inside a microwaveable container with perlite and water underneath it.



Your not going to get a large fruitbody from an agar plate. Not enough water. You would clone pins that pop up to keep those excellent genetics, but there is no way you will see anything substantial from growing it out.

You have a lot of reading to do. Good luck.


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Re: PDA petri dish pins [Re: Pastywhyte]
    #19381925 - 01/07/14 11:20 AM (10 years, 24 days ago)

I agree with pasty on all of the above.

Start off with pf tek and follow it to a T! Roger rabbit has a free, very instructional video if you haven't already seen it.

Experimenting is fun, but often to no avail until you have the basic understanding of how and why the growth process happens. I'm learning new stuff on this forum every day.


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



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Re: PDA petri dish pins [Re: MarcusFreeman]
    #19382203 - 01/07/14 12:24 PM (10 years, 23 days ago)



Let em grow out till the drop spores then use the spores for spore swabs. More clean than swabbing fruits that grew in a fruiting chamber. :thumbup:


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Re: PDA petri dish pins [Re: Pastywhyte]
    #19383297 - 01/07/14 03:55 PM (10 years, 23 days ago)

Quote:

Pastywhyte said:
Your not going to get a large fruitbody from an agar plate. Not enough water. You would clone pins that pop up to keep those excellent genetics, but there is no way you will see anything substantial from growing it out.




True enough.  The plates I originally plated out my pins from, I let them keep growing to see what happened:  Skinny stems, flimsy caps, and the whole thing just sort of fell over and turned to goop after it got too heavy to support itself.  Neat to watch from an experimental perspective.  Sad and gross from an "aw, poor mushie" perspective.


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Re: PDA petri dish pins [Re: Pastywhyte]
    #19383882 - 01/07/14 05:25 PM (10 years, 23 days ago)

Yeah seriously, pancake mix



The only reason it messed up is because it was too wet not because of the pancake mix, the pancake mix worked just fine.


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Re: PDA petri dish pins [Re: supernewfie]
    #19384276 - 01/07/14 06:35 PM (10 years, 23 days ago)

Not disputing the fact that it could work,  just wondering why you wouldn't bother following a tek to the letter when first starting out. Seems like your lack of following procedure seems to be your issue. Pancake cakes with too much ms solution, inoculating plates with none available to transfer to, putting unsterilzed or at least improperly sterilized media into a plate with the hopes of growing a crop thereby ruining any chance to used that plate for anything useful.  I guess I cant see your end game with all this :shrug:


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Re: PDA petri dish pins [Re: Pastywhyte]
    #19384328 - 01/07/14 06:44 PM (10 years, 23 days ago)

Quote:

Pastywhyte said:
Not disputing the fact that it could work,  just wondering why you wouldn't bother following a tek to the letter when first starting out. Seems like your lack of following procedure seems to be your issue. Pancake cakes with too much ms solution, inoculating plates with none available to transfer to, putting unsterilzed or at least improperly sterilized media into a plate with the hopes of growing a crop thereby ruining any chance to used that plate for anything useful.  I guess I cant see your end game with all this :shrug:



Some people have to learn for themselves rather than saving themselves the hassle at the expense others have already paid.


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Re: PDA petri dish pins [Re: bodhisatta]
    #19385049 - 01/07/14 08:59 PM (10 years, 23 days ago)

Quote:

bodhisatta said:
Quote:

Pastywhyte said:
Not disputing the fact that it could work,  just wondering why you wouldn't bother following a tek to the letter when first starting out. Seems like your lack of following procedure seems to be your issue. Pancake cakes with too much ms solution, inoculating plates with none available to transfer to, putting unsterilzed or at least improperly sterilized media into a plate with the hopes of growing a crop thereby ruining any chance to used that plate for anything useful.  I guess I cant see your end game with all this :shrug:



Some people have to learn for themselves rather than saving themselves the hassle at the expense others have already paid.




That's true, I'm the very same way. Only difference is that I only started doing that once I had a few dozen grows under my belt to give me a grounding in proper tek first.


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Re: PDA petri dish pins [Re: supernewfie]
    #19402764 - 01/11/14 08:31 AM (10 years, 20 days ago)





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Re: PDA petri dish pins [Re: supernewfie]
    #19407681 - 01/12/14 10:18 AM (10 years, 19 days ago)

Quote:

supernewfie said:







I guess I don't really know wtf I'm looking at but it doesn't make me happy. Why are there petri dishes ontop of perlite in a tupperware. Wet perlite does 0 for humidity if it doesn't have air moving through it to wick off the moisture. Shrooms will grow just fine off of petri dishes if you just leave them covered, but either way you're only going to get dinky ass shrooms if you try to fruit a dish.



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Re: PDA petri dish pins [Re: supernewfie]
    #19418639 - 01/14/14 05:57 PM (10 years, 16 days ago)





This is the brf/agar-type experiment going good so far, atleast i think..


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Re: PDA petri dish pins [Re: supernewfie]
    #19423132 - 01/15/14 02:29 PM (10 years, 15 days ago)



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Re: PDA petri dish pins [Re: supernewfie]
    #19423145 - 01/15/14 02:32 PM (10 years, 15 days ago)

:thumbup:


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Re: PDA petri dish pins [Re: cronicr]
    #19423178 - 01/15/14 02:37 PM (10 years, 15 days ago)

So cronicr, should I wait till the surface is 100% colonized before I can inoculate jars with it?

I read your post and you said it wasnt quite fully colonized when you transferred it..  mine is very liquidy so I might have to wait till it dries a bit and stiffins up..


Edited by supernewfie (01/15/14 02:42 PM)


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Re: PDA petri dish pins [Re: supernewfie]
    #19423204 - 01/15/14 02:41 PM (10 years, 15 days ago)

If it's clean wait till you got enough to be satisfied with


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Re: PDA petri dish pins [Re: cronicr]
    #19426652 - 01/16/14 08:02 AM (10 years, 15 days ago)

Thanks cronicr. I think I may transfer a few more pins over to some.. never quite followed instructions because I had no brf so I used pancake mix but all is well so far and it should stay clean I sterilized it. Going to get brf sometime soon and see if I can observe any noticeable differences in growth. Right now though it seems the pancake mix is working just fine from the amount of growth in the last 3 days (since I put the 2 pins in). Excellent idea though for sure for anyone without agar.


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Re: PDA petri dish pins [Re: supernewfie]
    #19453623 - 01/21/14 04:20 PM (10 years, 9 days ago)



Lol.. an old agar dish I had... I fed it some hydrated straw. It was rabbit food as a product :/ anyways when it colonized the straw I cased it and 2 days ago I noticed pins popping out....


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Re: PDA petri dish pins [Re: supernewfie]
    #19463338 - 01/23/14 04:12 PM (10 years, 7 days ago)



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Re: PDA petri dish pins [Re: supernewfie]
    #19463531 - 01/23/14 04:57 PM (10 years, 7 days ago)

:eek:


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Re: PDA petri dish pins [Re: cronicr]
    #19463569 - 01/23/14 05:05 PM (10 years, 7 days ago)

Cronicr, just wondering is that strictipes in your avatar picture?


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Re: PDA petri dish pins [Re: supernewfie]
    #19463631 - 01/23/14 05:17 PM (10 years, 7 days ago)

nope it's an outdoor pelliculosa:thumbup:


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Re: PDA petri dish pins [Re: cronicr]
    #19465512 - 01/23/14 11:48 PM (10 years, 7 days ago)

Quote:

cronicr said:
nope it's an outdoor pelliculosa:thumbup:



good to know. I'd wondered that myself back when you first changed it to that :grin:


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Re: PDA petri dish pins [Re: Psilosopherr]
    #19470965 - 01/25/14 08:07 AM (10 years, 6 days ago)



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Re: PDA petri dish pins [Re: supernewfie]
    #19496152 - 01/30/14 12:11 PM (10 years, 1 day ago)



I cut a nice chunk out as you can see

now about a week after its almost reached all sides and the part I cut out is almost grew back

no visible contams as of yet







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Re: PDA petri dish pins [Re: supernewfie]
    #19496304 - 01/30/14 12:59 PM (10 years, 23 hours ago)

looking good, i forgot i pulled my brf/ water subs out last week and now they all pinned on me, gonna drop some pins on proper plates tonight


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Re: PDA petri dish pins [Re: cronicr]
    #19496596 - 01/30/14 02:04 PM (10 years, 22 hours ago)

if I keep this particular mycelium going for example let these pin then grow out pins and when they pin use those and grow out more will it tire the strain after awhile, making it weaker and prone to not fruit? if so how long or how many generations of pinning coud I keep it going for. I read a few times about the strain loosing potential if you keep it going for so long without taking spores just using clone after clone..


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Re: PDA petri dish pins [Re: supernewfie]
    #19496641 - 01/30/14 02:12 PM (10 years, 22 hours ago)

it will just stop producing pins, just take one and drop it on a new plate and use that for your culture


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Re: PDA petri dish pins [Re: cronicr]
    #19510364 - 02/02/14 10:41 AM (9 years, 11 months ago)


Got some straw colonizing

Ya petri dish mushrooms are pretty scronny fer sure lol





Biggest one so far lol
.

.


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Re: PDA petri dish pins [Re: supernewfie]
    #19510418 - 02/02/14 10:56 AM (9 years, 11 months ago)

:eek:


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Re: PDA petri dish pins [Re: cronicr]
    #19510574 - 02/02/14 11:30 AM (9 years, 11 months ago)

Pretty nice :mushroom2:


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Re: PDA petri dish pins [Re: L1ss3rd]
    #19525016 - 02/05/14 11:48 AM (9 years, 11 months ago)

Don't know why im keeping this thread alive with pictures of my vast noob-like antics , but started 3 jars yesterday with cuttings of the clone I grew out, used very large portions of mycelium, figured the bigger the better... out of the 3 jars, youll see I have used most of the mycelium for them. this is whats left of the mycelium I have



here's 2 out of 3 of my jars. 2 are actually glasses and one is a tall half pint canning jar that's about halfway full with the cake mixture(last picture)





hopefully il see some good results :smirk: ... hopefully!!


Edited by supernewfie (02/05/14 12:27 PM)


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Re: PDA petri dish pins [Re: supernewfie]
    #19525055 - 02/05/14 11:54 AM (9 years, 11 months ago)

y scalpel them?


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Re: PDA petri dish pins [Re: cronicr]
    #19525064 - 02/05/14 11:56 AM (9 years, 11 months ago)

Why is there foil on the jars?


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Re: PDA petri dish pins [Re: Pastywhyte]
    #19525088 - 02/05/14 12:01 PM (9 years, 11 months ago)

theres tin foil on the glasses because theyre glasses,,,,, without it theyd be open and the jar I have tin foil on it still as an extra precaution to keep out contaminates. you think I should remove the foil from the half pint jarÉ and cronicr what do you mean y scalpel themÉ

keyboard wont make question marks,,, don't know how to change the mode its in:confused:


Edited by supernewfie (02/05/14 12:05 PM)


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Re: PDA petri dish pins [Re: supernewfie]
    #19525103 - 02/05/14 12:04 PM (9 years, 11 months ago)

i just wanted to know if he meant like tare the little pins or use the scalpel to simply remove them:justdontknow:


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Re: PDA petri dish pins [Re: cronicr]
    #19525120 - 02/05/14 12:08 PM (9 years, 11 months ago)

oh, no ive cut mycelium out of the container I had the pin growing out in, I never done anything with pins since that container, however, I have pins growing from a couple other dishes and may transfer over to another container to grow out like this one...

when I took this pin from the dish first I pierced the base with a needle and while still on the needle I transferred to the container

flame sterilized the needle as well and done all this inside a SAB

Quote:

Pastywhyte said:
Why is there foil on the jars?




the glasses I cant remove, but you think I should remove the foil from the canning jarÉ


Edited by supernewfie (02/05/14 12:29 PM)


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Re: PDA petri dish pins [Re: supernewfie]
    #19525406 - 02/05/14 01:15 PM (9 years, 11 months ago)

Change your keyboard setting by going to the right of your taskbar....the one on the bottom of your screen...click the little picture of the keyboard...change it to ENG canadain, or american and you should be good:thumbup:


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Re: PDA petri dish pins [Re: 1down5up]
    #19525431 - 02/05/14 01:22 PM (9 years, 11 months ago)

this computer is different, I used to have one that had the keyboard symbol on bottom to switch between those but its not on this one I figure it may be in control panel but I haven't checked.. I know im lazy lol but thanks for trying to help!!:thumbup: :smile:


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Re: PDA petri dish pins [Re: supernewfie]
    #19525543 - 02/05/14 01:47 PM (9 years, 11 months ago)

fuckin windows 8 eh?  You can download a program to get your start menu back....unless you're too lazy :lol:


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Re: PDA petri dish pins [Re: 1down5up]
    #19525595 - 02/05/14 01:59 PM (9 years, 11 months ago)

Quote:

1down5up said:
fuckin windows 8 eh?  You can download a program to get your start menu back....unless you're too lazy :lol:




:smirk:
:ducklol:

Where is this program you speak ofÉ


edit: found it, ???? lol removed Canadian multi-lingual from it altogether :P never gonna change on me again!


Edited by supernewfie (02/05/14 02:30 PM)


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Re: PDA petri dish pins [Re: supernewfie]
    #19531817 - 02/06/14 05:30 PM (9 years, 11 months ago)




.


Edited by supernewfie (02/07/14 08:48 AM)


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Re: PDA petri dish pins [Re: supernewfie]
    #19536540 - 02/07/14 07:05 PM (9 years, 11 months ago)


.

.



.
im gonna put this thread to rest and start a new one in 2 or 3 days and take more pics then. I dont be handling them theyre on the shelf in my closet on top. i turned this one around and took pictures of all angles. I can view them without handling much. Anyways, till then.. peacee


Edited by supernewfie (02/07/14 07:26 PM)


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