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Offlinesupernewfie
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Re: PDA petri dish pins [Re: supernewfie]
    #19418639 - 01/14/14 05:57 PM (10 years, 16 days ago)





This is the brf/agar-type experiment going good so far, atleast i think..


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: supernewfie]
    #19423132 - 01/15/14 02:29 PM (10 years, 15 days ago)



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OfflinecronicrFacebook
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Re: PDA petri dish pins [Re: supernewfie]
    #19423145 - 01/15/14 02:32 PM (10 years, 15 days ago)

:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: cronicr]
    #19423178 - 01/15/14 02:37 PM (10 years, 15 days ago)

So cronicr, should I wait till the surface is 100% colonized before I can inoculate jars with it?

I read your post and you said it wasnt quite fully colonized when you transferred it..  mine is very liquidy so I might have to wait till it dries a bit and stiffins up..


Edited by supernewfie (01/15/14 02:42 PM)


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OfflinecronicrFacebook
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Re: PDA petri dish pins [Re: supernewfie]
    #19423204 - 01/15/14 02:41 PM (10 years, 15 days ago)

If it's clean wait till you got enough to be satisfied with


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: cronicr]
    #19426652 - 01/16/14 08:02 AM (10 years, 15 days ago)

Thanks cronicr. I think I may transfer a few more pins over to some.. never quite followed instructions because I had no brf so I used pancake mix but all is well so far and it should stay clean I sterilized it. Going to get brf sometime soon and see if I can observe any noticeable differences in growth. Right now though it seems the pancake mix is working just fine from the amount of growth in the last 3 days (since I put the 2 pins in). Excellent idea though for sure for anyone without agar.


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: supernewfie]
    #19453623 - 01/21/14 04:20 PM (10 years, 9 days ago)



Lol.. an old agar dish I had... I fed it some hydrated straw. It was rabbit food as a product :/ anyways when it colonized the straw I cased it and 2 days ago I noticed pins popping out....


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: supernewfie]
    #19463338 - 01/23/14 04:12 PM (10 years, 7 days ago)



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OfflinecronicrFacebook
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Re: PDA petri dish pins [Re: supernewfie]
    #19463531 - 01/23/14 04:57 PM (10 years, 7 days ago)

:eek:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: cronicr]
    #19463569 - 01/23/14 05:05 PM (10 years, 7 days ago)

Cronicr, just wondering is that strictipes in your avatar picture?


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OfflinecronicrFacebook
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Re: PDA petri dish pins [Re: supernewfie]
    #19463631 - 01/23/14 05:17 PM (10 years, 7 days ago)

nope it's an outdoor pelliculosa:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinePsilosopherr
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Re: PDA petri dish pins [Re: cronicr]
    #19465512 - 01/23/14 11:48 PM (10 years, 7 days ago)

Quote:

cronicr said:
nope it's an outdoor pelliculosa:thumbup:



good to know. I'd wondered that myself back when you first changed it to that :grin:


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: Psilosopherr]
    #19470965 - 01/25/14 08:07 AM (10 years, 6 days ago)



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Offlinesupernewfie
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Re: PDA petri dish pins [Re: supernewfie]
    #19496152 - 01/30/14 12:11 PM (10 years, 23 hours ago)



I cut a nice chunk out as you can see

now about a week after its almost reached all sides and the part I cut out is almost grew back

no visible contams as of yet







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Re: PDA petri dish pins [Re: supernewfie]
    #19496304 - 01/30/14 12:59 PM (10 years, 22 hours ago)

looking good, i forgot i pulled my brf/ water subs out last week and now they all pinned on me, gonna drop some pins on proper plates tonight


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: cronicr]
    #19496596 - 01/30/14 02:04 PM (10 years, 21 hours ago)

if I keep this particular mycelium going for example let these pin then grow out pins and when they pin use those and grow out more will it tire the strain after awhile, making it weaker and prone to not fruit? if so how long or how many generations of pinning coud I keep it going for. I read a few times about the strain loosing potential if you keep it going for so long without taking spores just using clone after clone..


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Re: PDA petri dish pins [Re: supernewfie]
    #19496641 - 01/30/14 02:12 PM (10 years, 21 hours ago)

it will just stop producing pins, just take one and drop it on a new plate and use that for your culture


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: cronicr]
    #19510364 - 02/02/14 10:41 AM (9 years, 11 months ago)


Got some straw colonizing

Ya petri dish mushrooms are pretty scronny fer sure lol





Biggest one so far lol
.

.


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Re: PDA petri dish pins [Re: supernewfie]
    #19510418 - 02/02/14 10:56 AM (9 years, 11 months ago)

:eek:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineL1ss3rd
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Re: PDA petri dish pins [Re: cronicr]
    #19510574 - 02/02/14 11:30 AM (9 years, 11 months ago)

Pretty nice :mushroom2:


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