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Offlinesupernewfie
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Re: PDA petri dish pins [Re: CliftonGK1]
    #19378807 - 01/06/14 07:07 PM (10 years, 24 days ago)

I have no agar and I would probably have to order it online

I may try the brf agar-type experiment


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Re: PDA petri dish pins [Re: supernewfie]
    #19378950 - 01/06/14 07:35 PM (10 years, 24 days ago)

:thumbup:


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Re: PDA petri dish pins [Re: MarcusFreeman]
    #19379504 - 01/06/14 09:16 PM (10 years, 24 days ago)

Quote:

MarcusFreeman said:
https://www.shroomery.org/forums/showflat.php/Number/19296230

He made an "agar" out of brf and water..... check it out




funny was just reading this earlyer


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: supernewfie]
    #19381200 - 01/07/14 07:33 AM (10 years, 24 days ago)

Its growing so quickly I think im going to leave this one il transfer pins from another dish to clone I just want to watch the progress of this one. My first grow ever could you blame me lol, it just looks soo fucking awesome :p



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Offlinesupernewfie
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Re: PDA petri dish pins [Re: supernewfie]
    #19381546 - 01/07/14 09:43 AM (10 years, 24 days ago)

:o I put it in yesterday, its fuzzing up and looks like it will take to the cookie!!



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Re: PDA petri dish pins [Re: supernewfie]
    #19381583 - 01/07/14 09:53 AM (10 years, 24 days ago)

So... why is that grain in the dish?

The agar should have all the nutrient needed for growth and cloning. Adding grain and frequently opening the Petris will increase the chance of contams getting on the grain and agar.


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



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Offlinesupernewfie
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Re: PDA petri dish pins [Re: MarcusFreeman]
    #19381641 - 01/07/14 10:12 AM (10 years, 24 days ago)

I boiled the grain and treated it like agar in a way and transferred it to the dish and when it cooled I transferred the mushroom onto it and taped it down on 2 sides... I havent got agar, I will try the brf agar with another pin forming on a different petri...


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: MarcusFreeman]
    #19381664 - 01/07/14 10:18 AM (10 years, 24 days ago)

Ohh the rye seed, I spread some on hoping the mycelium would take to it to give it a bit of extra nutrition.. I figured it might support the mushrooms growth? Maybe not though, and I have the cover completely off now, inside a microwaveable container with perlite and water underneath it.


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Re: PDA petri dish pins [Re: supernewfie]
    #19381761 - 01/07/14 10:42 AM (10 years, 24 days ago)

Well, that grain probably won't do a whole lot to support the mushy growth at this point. The mycelium would have already needed to break it down and have it ready to be used as energy for growth. Since it already has most of its energy focused on  reproduction, it likely won't benefit from added nutrient.

You say this is your first grow. Have you not done a PF cake yet?


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



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Offlinesupernewfie
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Re: PDA petri dish pins [Re: MarcusFreeman]
    #19381787 - 01/07/14 10:48 AM (10 years, 24 days ago)

Ive done a cake and it ruined way to wet.. verm and organic pancake mix it started awesome but I shot way too much spore water in. Had to dump it yesterday smelled aweful


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Re: PDA petri dish pins [Re: supernewfie]
    #19381892 - 01/07/14 11:13 AM (10 years, 24 days ago)

Quote:

supernewfie said:
I boiled the grain and treated it like agar in a way and transferred it to the dish and when it cooled I transferred the mushroom onto it and taped it down on 2 sides.



I will be shocked if that does not contam.

Quote:

supernewfie said:
Ive done a cake and it ruined way to wet.. verm and organic pancake mix it started awesome but I shot way too much spore water in. Had to dump it yesterday smelled aweful



Seriously,  pancake mix? :wow:

Quote:

supernewfie said:
Ohh the rye seed, I spread some on hoping the mycelium would take to it to give it a bit of extra nutrition.. I figured it might support the mushrooms growth? Maybe not though, and I have the cover completely off now, inside a microwaveable container with perlite and water underneath it.



Your not going to get a large fruitbody from an agar plate. Not enough water. You would clone pins that pop up to keep those excellent genetics, but there is no way you will see anything substantial from growing it out.

You have a lot of reading to do. Good luck.


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Re: PDA petri dish pins [Re: Pastywhyte]
    #19381925 - 01/07/14 11:20 AM (10 years, 24 days ago)

I agree with pasty on all of the above.

Start off with pf tek and follow it to a T! Roger rabbit has a free, very instructional video if you haven't already seen it.

Experimenting is fun, but often to no avail until you have the basic understanding of how and why the growth process happens. I'm learning new stuff on this forum every day.


--------------------
"The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK

As one ends, another begins.



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Re: PDA petri dish pins [Re: MarcusFreeman]
    #19382203 - 01/07/14 12:24 PM (10 years, 24 days ago)



Let em grow out till the drop spores then use the spores for spore swabs. More clean than swabbing fruits that grew in a fruiting chamber. :thumbup:


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OfflineCliftonGK1
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Re: PDA petri dish pins [Re: Pastywhyte]
    #19383297 - 01/07/14 03:55 PM (10 years, 23 days ago)

Quote:

Pastywhyte said:
Your not going to get a large fruitbody from an agar plate. Not enough water. You would clone pins that pop up to keep those excellent genetics, but there is no way you will see anything substantial from growing it out.




True enough.  The plates I originally plated out my pins from, I let them keep growing to see what happened:  Skinny stems, flimsy caps, and the whole thing just sort of fell over and turned to goop after it got too heavy to support itself.  Neat to watch from an experimental perspective.  Sad and gross from an "aw, poor mushie" perspective.


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: Pastywhyte]
    #19383882 - 01/07/14 05:25 PM (10 years, 23 days ago)

Yeah seriously, pancake mix



The only reason it messed up is because it was too wet not because of the pancake mix, the pancake mix worked just fine.


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Re: PDA petri dish pins [Re: supernewfie]
    #19384276 - 01/07/14 06:35 PM (10 years, 23 days ago)

Not disputing the fact that it could work,  just wondering why you wouldn't bother following a tek to the letter when first starting out. Seems like your lack of following procedure seems to be your issue. Pancake cakes with too much ms solution, inoculating plates with none available to transfer to, putting unsterilzed or at least improperly sterilized media into a plate with the hopes of growing a crop thereby ruining any chance to used that plate for anything useful.  I guess I cant see your end game with all this :shrug:


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Re: PDA petri dish pins [Re: Pastywhyte]
    #19384328 - 01/07/14 06:44 PM (10 years, 23 days ago)

Quote:

Pastywhyte said:
Not disputing the fact that it could work,  just wondering why you wouldn't bother following a tek to the letter when first starting out. Seems like your lack of following procedure seems to be your issue. Pancake cakes with too much ms solution, inoculating plates with none available to transfer to, putting unsterilzed or at least improperly sterilized media into a plate with the hopes of growing a crop thereby ruining any chance to used that plate for anything useful.  I guess I cant see your end game with all this :shrug:



Some people have to learn for themselves rather than saving themselves the hassle at the expense others have already paid.


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Re: PDA petri dish pins [Re: bodhisatta]
    #19385049 - 01/07/14 08:59 PM (10 years, 23 days ago)

Quote:

bodhisatta said:
Quote:

Pastywhyte said:
Not disputing the fact that it could work,  just wondering why you wouldn't bother following a tek to the letter when first starting out. Seems like your lack of following procedure seems to be your issue. Pancake cakes with too much ms solution, inoculating plates with none available to transfer to, putting unsterilzed or at least improperly sterilized media into a plate with the hopes of growing a crop thereby ruining any chance to used that plate for anything useful.  I guess I cant see your end game with all this :shrug:



Some people have to learn for themselves rather than saving themselves the hassle at the expense others have already paid.




That's true, I'm the very same way. Only difference is that I only started doing that once I had a few dozen grows under my belt to give me a grounding in proper tek first.


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Offlinesupernewfie
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Re: PDA petri dish pins [Re: supernewfie]
    #19402764 - 01/11/14 08:31 AM (10 years, 20 days ago)





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Re: PDA petri dish pins [Re: supernewfie]
    #19407681 - 01/12/14 10:18 AM (10 years, 19 days ago)

Quote:

supernewfie said:







I guess I don't really know wtf I'm looking at but it doesn't make me happy. Why are there petri dishes ontop of perlite in a tupperware. Wet perlite does 0 for humidity if it doesn't have air moving through it to wick off the moisture. Shrooms will grow just fine off of petri dishes if you just leave them covered, but either way you're only going to get dinky ass shrooms if you try to fruit a dish.



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