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MightyMustache
Limburgian

Registered: 08/10/10
Posts: 197
Last seen: 9 years, 4 months
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A few general questions
#19369979 - 01/04/14 07:36 PM (10 years, 26 days ago) |
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So, about a year ago i had some fresh magic truffles. Had a great time with them, enough to misplace the (nearly empty) plastic container the truffles came in. Few weeks later i stumbled upon said container, with (what probably was) mycelium growing richly on the few stones that were left in it.
Now i have a interest in biology and psychedelics and thought "hey, might read up on the subject and have some fun with this" but i never acted on it. Until i recently moved and again stumbled on that little plastic box (stones bone dry by then off course).
Now they might just have had cobweb or mildew growing on them, and off course old dried "dirty" stones are not the best material to clone. And doing so via a LC instead of agar will probably turn into contam galore if anything, but i thought fuck it, why not. Got a trip (and thus access to fresh stones to take a actual sterile-ish sample from) planned soon anyways. So i pressure cooked 3 jars of honey based LC, added the very well washed stones. Guess they might as well re-hydrate in the LC instead of before introducing them. And put them aside to hopefully do their thing.
Off course it's hard to rule out contaminants with a LC, but after a day or 2 or 3 i am definitely noticing mycelium like tissue growing on top of the stones. No floating "oil slicks", jelly like substances or fancy colors going on, so it looks clean.
Now for the questions, loooaaads of information to find, but I'd like some opinions before i go ahead with a test jar and eventually cloning the fresh stones.:
(as a little disclaimer, kind of a "nothing to lose" grow, just using stuff i have laying around anyways. Fully aware there are superior techniques and materials.)
On inoculating: Is there a huge risk contam wise if you work in a draft free sanitized space? If i turn a box/cupboard into a imaginary BSL-3 room, would there be a 90% rate of failure or am i pretty safe just knocking up a jar or 2? Note that im going the sloppy way by introducing a sterilized spoon instead of a syringe.
The substrate. I got a choice of WBS, mixed grain (the feed kind, which contains corn, a no no?) and ordinary everyday lawn grass seeds. Read plenty about WBS and fungicide free grass seeds working fine. Any mayor differences between those growth wise and which would you go with?
Also could adding inorganic material help? As in providing sites to develop sclerotia, or am i just fantasizing there? Other helpful substances besides hydrogen peroxide/coffee?
Oh and I'm using "golden teachers", not sure which (sub?)strain besides cubensis. Im guessing since they are sold as stones, the genetics to form them are there too. Not fruiing either, strictly jars.
Edited by MightyMustache (01/04/14 07:41 PM)
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MightyMustache
Limburgian

Registered: 08/10/10
Posts: 197
Last seen: 9 years, 4 months
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One of the donor stones has gotten all fizzy. Tiny bubbles on the surface of the stone and some caught in the mycelium.
Gas produced by the mycelium? Or a problem?
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,059
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Quote:
MightyMustache said: One of the donor stones has gotten all fizzy. Tiny bubbles on the surface of the stone and some caught in the mycelium.
Gas produced by the mycelium? Or a problem?
I did a quick search for this. It looks like you may be in uncharted territory. As always pictures are worth a thousand words. However I did have a 'bubble' like contamination which I think was yeast. Was not a good sign. Keep us posted?
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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MightyMustache
Limburgian

Registered: 08/10/10
Posts: 197
Last seen: 9 years, 4 months
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Yeah i inoculated the LC's about 4 days ago, it's just a cm or 2 of (presumably) mycelium growing on top/around the stones. I'll try to snap a picture when it's more visible. (suck at macro's)
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MightyMustache
Limburgian

Registered: 08/10/10
Posts: 197
Last seen: 9 years, 4 months
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Not the best pic, the "fizzyness" is not really visible, but here you go: Mr bubbles
Edited by MightyMustache (01/05/14 10:34 AM)
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