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TheUnknownPoet
Stranger



Registered: 11/14/12
Posts: 349
Last seen: 8 years, 8 months
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Did I fuck up? (Peeked at my tub)
#19369778 - 01/04/14 06:54 PM (10 years, 1 month ago) |
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So my monotub was accidentally left near a heating vent, and one side got very hot, so today i removed it and peeked inside hoping bacteria hadn't formed.
There was no bacteria, and most of the top was colonised (Not completely colonised, but most of the surface had light mycellium on it)
is my monotub going to contaminate since i opened it now?
i made 1 other monotub with the bucket tek, and that contaminated after i peeked. this tub was properly pasteurized though. will it contaminate since i peeked once to check for bacteria? It smelled fine and there was no bacteria.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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lol no your peeking isn't contaminating your sub
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mastercultivator
Master Cultivator



Registered: 08/27/13
Posts: 653
Last seen: 7 months, 27 days
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If anything the heating vent helped it colonize quicker or at least on time.
Blowing hot air on your tubs will totally help them along as long as they don't get too hot. 84F is ok for tubs. The center of the sub might get hotter though for larger subs and subs made with straw so watch out for those conditions.
Your tub will be fine even though you peeked at it.
But to answer your question in more depth as to fear of contaminants.
You should get into a regimen of spraying with a 0.03% solution of H202. Mix 10ml of 3% H202 you buy at the store with 1L of water, spring, or tap in most cases. ( As long as your tap water isn't gross or rusty or something knarly like fracking water that you can light on fire ). Just spray with that solution as heavily as you like just cover everything with it. Even if some dust falls in the bad fungi and bacteria won't even get a start.
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    I'm awesome sauce with a dash of sunshine.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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 don't use h2o2 for anything but fighting cobweb mold, leave your sub alone a proper pasturizing is all yopu need and a fully colonized sub is contam resistent, lots of fresh air full colonization and a proper pasturization are your best h=bets against the trich wars
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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lessismore
Registered: 02/10/13
Posts: 6,268
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Re: Did I fuck up? (Peeked at my tub) [Re: cronicr]
#19369948 - 01/04/14 07:29 PM (10 years, 1 month ago) |
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Quote:
cronicr said:
 don't use h2o2 for anything but fighting cobweb mold, leave your sub alone a proper pasturizing is all yopu need and a fully colonized sub is contam resistent, lots of fresh air full colonization and a proper pasturization are your best h=bets against the trich wars
dont peek if you dont have an extra clear trashbag in the top of the tub to peek through
else you might introduce contams most likely? - as far as I remember, but search it
one time I had forgotten to close the tub completely, it contamed with black mold 
if seeing any contams, I would toss it I dont try to save if I see a contamed tub, not worth it IMO that tub got pinmolds + black molds, not a lot, but tossed it quickly anyway
it was 99% colonized anyway, but pinmold on top
also... listen to this guy... h2o2 is nogo, bad idea
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: Did I fuck up? (Peeked at my tub) [Re: lessismore]
#19369959 - 01/04/14 07:31 PM (10 years, 1 month ago) |
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Quote:
mio said: dont peek if you dont have an extra clear trashbag in the top of the tub to peek through
else you might introduce contams most likely?
The tub was most likley spawned in open air, meaning there are already millions of contams mixed into the substrate.
Worrying about contams at this point is silly.
Peeking is fine.....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Did I fuck up? (Peeked at my tub) [Re: lessismore]
#19369965 - 01/04/14 07:32 PM (10 years, 1 month ago) |
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Quote:
mastercultivator said: If anything the heating vent helped it colonize quicker or at least on time.
Blowing hot air on your tubs will totally help them along as long as they don't get too hot. 84F is ok for tubs. The center of the sub might get hotter though for larger subs and subs made with straw so watch out for those conditions.
Your tub will be fine even though you peeked at it.
But to answer your question in more depth as to fear of contaminants.
You should get into a regimen of spraying with a 0.03% solution of H202. Mix 10ml of 3% H202 you buy at the store with 1L of water, spring, or tap in most cases. ( As long as your tap water isn't gross or rusty or something knarly like fracking water that you can light on fire ). Just spray with that solution as heavily as you like just cover everything with it. Even if some dust falls in the bad fungi and bacteria won't even get a start.
75F is the generally recommended best temperature 84F is way to hot for colonization.
h2o2 is fail
Quote:
mio said:
Quote:
cronicr said:
 don't use h2o2 for anything but fighting cobweb mold, leave your sub alone a proper pasturizing is all yopu need and a fully colonized sub is contam resistent, lots of fresh air full colonization and a proper pasturization are your best h=bets against the trich wars
dont peek if you dont have an extra clear trashbag in the top of the tub to peek through
else you might introduce contams most likely? - as far as I remember, but search it
one time I had forgotten to close the tub completely, it contamed with black mold 
if seeing any contams, I would toss it I dont try to save if I see a contamed tub, not worth it IMO that tub got pinmolds + black molds, not a lot, but tossed it quickly anyway
it was 99% colonized anyway, but pinmold on top
also... listen to this guy... h2o2 is nogo, bad idea
You can peek without a clear lid. You were peeking at it the whole time you mixed your spawn with your substrate...
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Did I fuck up? (Peeked at my tub) [Re: lessismore]
#19369970 - 01/04/14 07:33 PM (10 years, 1 month ago) |
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the fact of the matter is if you pasturized your sub properly you would be able to leave it out in the open for 2 weeks with out it going moldy, we keep it in our tubs for high c02 levels and moisture control, peeking isn't gonna harm your tubs you should peek every few days as far as i'm concerned
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Did I fuck up? (Peeked at my tub) [Re: cronicr]
#19370126 - 01/04/14 08:11 PM (10 years, 1 month ago) |
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Quote:
cronicr said: the fact of the matter is if you pasturized your sub properly you would be able to leave it out in the open for 2 weeks with out it going moldy, we keep it in our tubs for high c02 levels and moisture control, peeking isn't gonna harm your tubs you should peek every few days as far as i'm concerned

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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Did I fuck up? (Peeked at my tub) [Re: bodhisatta]
#19370249 - 01/04/14 08:53 PM (10 years, 1 month ago) |
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the only concern with peeking to much is you expose your sub to too much fresh air prier to colonization. Worrying about exposure to contams is silly at this point. Who ever is still using H202 should step out of the past. its well known these days that peroxide isn't good for much in this hobby.
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TheUnknownPoet
Stranger



Registered: 11/14/12
Posts: 349
Last seen: 8 years, 8 months
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Re: Did I fuck up? (Peeked at my tub) [Re: MudaFuka]
#19370276 - 01/04/14 09:00 PM (10 years, 1 month ago) |
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I use peroxide to disinfect cuts on my hands.
That's about it. That's about all i'll be using it for, certainly not mycology.
Peroxide agar is also used sometimes, but not in mycology- not for me anyways.
I'm just REALLY paranoid, because the first monotub I did, got contaminated. But i used the bucket tek. I'm hoping this tub will be fine, it really needs to be, or else i'm in a bit of trouble. (I dont have enougn money or resources to try again)
Anyways, thanks for the help guys, im just really paranoid about contamination.
Also, because i'm allergic to penecillin, (Any exposure to it would require a blood transfusion for me, or I will die) and i'm hoping if it does contaminate, it won't be penecilin mould. (I don't want to die.)
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