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Offlinesecretsolosoul
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Registered: 01/04/14
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Last seen: 10 years, 26 days
Possible dead spores from heat?
    #19368132 - 01/04/14 01:02 PM (10 years, 27 days ago)

I tried doing a search for this, and couldn't find the answer I was looking for..

I tried BRF tek method with amazonian. Now a month later, I have no mycelium growth.

I had not let the jars cool well enough, and may have killed the spores.
It was uncomfortable to hold in my hand for longer than 20 seconds.

Another possibility, but less likely is that they are growing slightly under room temperature.
But would this not just slow the growth, and given a month, see at least something? Keep in mind, I've seen no growth in contams either..

Now, only using half the syringe, what would be best?
Continue waiting?..
Re-sterilize the jars, cool appropriately and try again with current substrate?
Or, toss the mixture and start from scratch?

I'm a complete noob, sorry.
Any other suggestions are welcome. Thank you!


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
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Re: Possible dead spores from heat? [Re: secretsolosoul]
    #19368140 - 01/04/14 01:03 PM (10 years, 27 days ago)

start over, don't use the same jars and let them cool properly, for the few extra hours you should have waited you have now waited a month for nuthing lol


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InvisiblebodhisattaMDiscordReddit
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Registered: 04/30/13
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Loc: Milky way
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Re: Possible dead spores from heat? [Re: secretsolosoul]
    #19368147 - 01/04/14 01:05 PM (10 years, 27 days ago)

If you couldn't hold the jar for 20s the inside of it was probably too hot and you fried the spores is my guess. Let them cool over night take the foil off right away and while it cools it sets up your dry verm barrier, Inoculate in a SAB on the next day.


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