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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
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Chaga fruiting on the plate!
#19366581 - 01/04/14 01:10 AM (10 years, 1 month ago) |
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Crazy as hell! This chaga was cloned from fresh sclerotia tissue and has now made fertile polypores in vitro. Dropping spores like mad. Dunno what to do next exactly. Agar is mypa. Might make a bunch of multispore plates from one of these.
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theonceandfuture
Young Man of the Woods
Registered: 12/11/13
Posts: 41
Last seen: 7 years, 4 months
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Holy shit! Now I've seen it all! Amazing stuff, keep up the good work!
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Shu
Vote for Humanity



Registered: 11/20/11
Posts: 885
Loc: PA, USA
Last seen: 4 days, 18 hours
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Very cool AV. At what temperature were the fruiting bodies produced?
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
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Re: Chaga fruiting on the plate! [Re: Shu]
#19367153 - 01/04/14 07:58 AM (10 years, 1 month ago) |
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The lab stays at about 65 F. These are at five weeks from original clone. Never subbed and somehow completely free of bacteria.
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Jeff
Addict



Registered: 10/06/12
Posts: 1,488
Last seen: 6 years, 2 months
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Neat stuff AV!
-------------------- Myco-tek
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 11 months
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Re: Chaga fruiting on the plate! [Re: Jeff]
#19367335 - 01/04/14 09:24 AM (10 years, 1 month ago) |
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wild! never seen a polypore pin on agar!
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: Chaga fruiting on the plate! [Re: drake89]
#19367438 - 01/04/14 09:59 AM (10 years, 1 month ago) |
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Whoa! Very fun!
--------------------
I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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Queen of Kings
Get on with the Fascination



Registered: 12/11/11
Posts: 1,201
Loc: Pacific Northwest, almost
Last seen: 7 years, 1 month
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Re: Chaga fruiting on the plate! [Re: Ganzig]
#19367450 - 01/04/14 10:02 AM (10 years, 1 month ago) |
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Very cool AV!
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
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What should I do y'all? Sub out little pieces of the fruit? Or multispore? My goal is to get vigorous Myc for plug innoc but I do not fully understand the life cycle.
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 18 days
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Looks like you've got an amazingly vigorous culture for your plugs - can't you just transfer parts of it that aren't fruits and use that to colonize your plugs? Sorry maybe I'm confused about what you're asking. That's an awesome culture though, mine sucks and grows super slow and doesn't do ANYTHING like that. So I wouldn't see any reason to go to spore, really...?
I was reading the other day chaga will often produce fruit bodies and spores when it's tree dies, as it's last chance to pass on genetics, so maybe your dish there felt threatened
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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OICU812
NC Tree Farm owner


Registered: 11/06/11
Posts: 1,064
Loc: Foothills of NC
Last seen: 3 years, 5 months
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Re: Chaga fruiting on the plate! [Re: Forrester]
#19368929 - 01/04/14 03:51 PM (10 years, 1 month ago) |
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Maybe try to take a few samples of the mycelium @ 5 o'clock in the picture above to get as far away from the spores as possible. Then get a few more samples at random areas and compare P2 plates, all done in a SAB, of course. It would be a shame to loose those original genetics!
-------------------- -------------- "Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin "Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin ----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 18 days
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Re: Chaga fruiting on the plate! [Re: OICU812]
#19369077 - 01/04/14 04:27 PM (10 years, 1 month ago) |
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Quote:
OICU812 said: Maybe try to take a few samples of the mycelium @ 5 o'clock in the picture above to get as far away from the spores as possible. Then get a few more samples at random areas and compare P2 plates, all done in a SAB, of course. It would be a shame to loose those original genetics!
Duh, the spores didn't even occur to me Good point.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
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Re: Chaga fruiting on the plate! [Re: Forrester]
#19369429 - 01/04/14 05:43 PM (10 years, 1 month ago) |
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Yea. The spores pretty much contaminate the original culture strain. This may not be a bad thing though. I with I would have subbed it prior.
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 18 days
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Quote:
Amanita virosa said: Yea. The spores pretty much contaminate the original culture strain. This may not be a bad thing though. I with I would have subbed it prior.
I would imagine a transfer of the culture (one as fast as yours) would outrun the spores before they could germinate and contaminate it, wouldn't you think?
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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OICU812
NC Tree Farm owner


Registered: 11/06/11
Posts: 1,064
Loc: Foothills of NC
Last seen: 3 years, 5 months
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Re: Chaga fruiting on the plate! [Re: Forrester]
#19369857 - 01/04/14 07:12 PM (10 years, 1 month ago) |
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Quote:
Forrester said: I would imagine a transfer of the culture (one as fast as yours) would outrun the spores before they could germinate and contaminate it, wouldn't you think?
That sounds like it's worth a shot, Av, maybe try a non-nutritive agar to make the Mycelium run even faster, and make the agar a little on the dry/firm side by adding a little extra agar agar powder.
*/* P.S.: When you have what you think is clean mycelium, isolate some of it to sterile distilled water for long term storage.
Edited by OICU812 (01/05/14 09:16 AM)
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
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Re: Chaga fruiting on the plate! [Re: OICU812]
#19369942 - 01/04/14 07:27 PM (10 years, 1 month ago) |
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Good idea y'all. Will sub away tomorrow. Don't wanna get too carried away on this project. But it was cool to see the fruiting bodies for sure. I had never seem them in nature.
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Group
Working Together


Registered: 10/25/13
Posts: 115
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Amazing!!!
-------------------- We are starting a mushroom growing network to provide FREE and/or inexpensive gourmet and medicinal mushrooms to local communities accross the US and globally. We have the opportunity to feed and heal each other. We simply need to work together. By sharing our talents we enable ourselves and others to enjoy a world of abundance. We'd love for collaboration from YOU, PM me!
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woodland_jewel
In den Gärten Pharaos


Registered: 09/07/11
Posts: 401
Loc: Penna
Last seen: 1 year, 7 months
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Re: Chaga fruiting on the plate! [Re: Group]
#19372693 - 01/05/14 12:27 PM (10 years, 1 month ago) |
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That's incredible, AV! Congrats!
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nycomyco
Stranger

Registered: 11/13/03
Posts: 651
Loc: PA
Last seen: 7 months, 4 days
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very cool! it's a privilege to see the fruitbodies. now who has the land to plant a birch forest- they grow very fast
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SnowArcher
Hood Rat


Registered: 12/30/13
Posts: 179
Last seen: 2 years, 11 months
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Re: Chaga fruiting on the plate! [Re: nycomyco]
#19373691 - 01/05/14 04:18 PM (10 years, 1 month ago) |
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Nice!!!
Was that the only plate?
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