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OfflineAmanita virosa
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Registered: 12/04/11
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Chaga fruiting on the plate!
    #19366581 - 01/04/14 01:10 AM (10 years, 1 month ago)



Crazy as hell!  This chaga was cloned from fresh sclerotia tissue and has now made fertile polypores in vitro. Dropping spores like mad. Dunno what to do next exactly. Agar is mypa. Might make a bunch of multispore plates from one of these.


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Offlinetheonceandfuture
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Re: Chaga fruiting on the plate! [Re: Amanita virosa]
    #19367076 - 01/04/14 07:06 AM (10 years, 1 month ago)

Holy shit! Now I've seen it all! Amazing stuff, keep up the good work!


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OfflineShu
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Re: Chaga fruiting on the plate! [Re: Amanita virosa]
    #19367116 - 01/04/14 07:37 AM (10 years, 1 month ago)

Very cool AV. At what temperature were the fruiting bodies produced?


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OfflineAmanita virosa
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Re: Chaga fruiting on the plate! [Re: Shu]
    #19367153 - 01/04/14 07:58 AM (10 years, 1 month ago)

The lab stays at about 65 F.  These are at five weeks from original clone. Never subbed and somehow completely free of bacteria.


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OfflineJeff
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Re: Chaga fruiting on the plate! [Re: Amanita virosa]
    #19367189 - 01/04/14 08:16 AM (10 years, 1 month ago)

Neat stuff AV!


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Myco-tek


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Offlinedrake89
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Re: Chaga fruiting on the plate! [Re: Jeff]
    #19367335 - 01/04/14 09:24 AM (10 years, 1 month ago)

wild!  never seen a polypore pin on agar!


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InvisibleGanzig
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Re: Chaga fruiting on the plate! [Re: drake89]
    #19367438 - 01/04/14 09:59 AM (10 years, 1 month ago)

Whoa! Very fun!


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OfflineQueen of Kings
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Re: Chaga fruiting on the plate! [Re: Ganzig]
    #19367450 - 01/04/14 10:02 AM (10 years, 1 month ago)

Very cool AV!


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OfflineAmanita virosa
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Re: Chaga fruiting on the plate! [Re: Queen of Kings]
    #19367513 - 01/04/14 10:23 AM (10 years, 1 month ago)

What should I do y'all?  Sub out little pieces of the fruit?  Or multispore?  My goal is to get vigorous Myc for plug innoc but I do not fully understand the life cycle.


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OfflineForresterM
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Re: Chaga fruiting on the plate! [Re: Amanita virosa]
    #19368195 - 01/04/14 01:13 PM (10 years, 1 month ago)

Looks like you've got an amazingly vigorous culture for your plugs - can't you just transfer parts of it that aren't fruits and use that to colonize your plugs?  Sorry maybe I'm confused about what you're asking.  That's an awesome culture though, mine sucks and grows super slow and doesn't do ANYTHING like that.  So I wouldn't see any reason to go to spore, really...?

I was reading the other day chaga will often produce fruit bodies and spores when it's tree dies, as it's last chance to pass on genetics, so maybe your dish there felt threatened :lol:


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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineOICU812
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Re: Chaga fruiting on the plate! [Re: Forrester]
    #19368929 - 01/04/14 03:51 PM (10 years, 1 month ago)

Maybe try to take a few samples of the mycelium @ 5 o'clock in the picture above to get as far away from the spores as possible.  Then get a few more samples at random areas and compare P2 plates, all done in a SAB, of course.  It would be a shame to loose those original genetics!


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OfflineForresterM
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Re: Chaga fruiting on the plate! [Re: OICU812]
    #19369077 - 01/04/14 04:27 PM (10 years, 1 month ago)

Quote:

OICU812 said:
Maybe try to take a few samples of the mycelium @ 5 o'clock in the picture above to get as far away from the spores as possible.  Then get a few more samples at random areas and compare P2 plates, all done in a SAB, of course.  It would be a shame to loose those original genetics!




Duh, the spores didn't even occur to me :facepalm:  Good point.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineAmanita virosa
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Re: Chaga fruiting on the plate! [Re: Forrester]
    #19369429 - 01/04/14 05:43 PM (10 years, 1 month ago)

Yea. The spores pretty much contaminate the original culture strain. This may not be a bad thing though. I with I would have subbed it prior.


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OfflineForresterM
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Re: Chaga fruiting on the plate! [Re: Amanita virosa]
    #19369659 - 01/04/14 06:29 PM (10 years, 1 month ago)

Quote:

Amanita virosa said:
Yea. The spores pretty much contaminate the original culture strain. This may not be a bad thing though. I with I would have subbed it prior.




I would imagine a transfer of the culture (one as fast as yours) would outrun the spores before they could germinate and contaminate it, wouldn't you think?


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineOICU812
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Re: Chaga fruiting on the plate! [Re: Forrester]
    #19369857 - 01/04/14 07:12 PM (10 years, 1 month ago)

Quote:

Forrester said:
I would imagine a transfer of the culture (one as fast as yours) would outrun the spores before they could germinate and contaminate it, wouldn't you think?




:thumbup: That sounds like it's worth a shot, Av, maybe try a non-nutritive agar to make the Mycelium run even faster, and make the agar a little on the dry/firm side by adding a little extra agar agar powder.

  */* P.S.:  When you have what you think is clean mycelium, isolate some of it to sterile distilled water for long term storage.



Edited by OICU812 (01/05/14 09:16 AM)


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OfflineAmanita virosa
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Re: Chaga fruiting on the plate! [Re: OICU812]
    #19369942 - 01/04/14 07:27 PM (10 years, 1 month ago)

Good idea y'all. Will sub away tomorrow. Don't wanna get too carried away on this project. But it was cool to see the fruiting bodies for sure. I had never seem them in nature.


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InvisibleGroup
Working Together


Registered: 10/25/13
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Re: Chaga fruiting on the plate! [Re: Amanita virosa]
    #19370599 - 01/04/14 10:32 PM (10 years, 1 month ago)

Amazing!!!


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We'd love for collaboration from YOU, PM me!


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Offlinewoodland_jewel
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Re: Chaga fruiting on the plate! [Re: Group]
    #19372693 - 01/05/14 12:27 PM (10 years, 1 month ago)

That's incredible, AV! Congrats!


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Offlinenycomyco
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Re: Chaga fruiting on the plate! [Re: woodland_jewel]
    #19373087 - 01/05/14 01:45 PM (10 years, 1 month ago)

very cool!  it's a privilege to see the fruitbodies.  now who has the land to plant a birch forest- they grow very fast :smile:


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OfflineSnowArcher
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Re: Chaga fruiting on the plate! [Re: nycomyco]
    #19373691 - 01/05/14 04:18 PM (10 years, 1 month ago)

Nice!!!

Was that the only plate?


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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Substrate Bags   Kraken Kratom Red Vein Kratom   Amanita Muscaria Store Amanita Muscaria   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies


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