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Amanita virosa
botanist by day


Registered: 12/04/11
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Loc: north kakalacky
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Chaga fruiting on the plate!
#19366581 - 01/04/14 01:10 AM (10 years, 27 days ago) |
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Crazy as hell! This chaga was cloned from fresh sclerotia tissue and has now made fertile polypores in vitro. Dropping spores like mad. Dunno what to do next exactly. Agar is mypa. Might make a bunch of multispore plates from one of these.
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theonceandfuture
Young Man of the Woods
Registered: 12/11/13
Posts: 41
Last seen: 7 years, 3 months
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Holy shit! Now I've seen it all! Amazing stuff, keep up the good work!
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Shu
Vote for Humanity



Registered: 11/20/11
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Loc: PA, USA
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Very cool AV. At what temperature were the fruiting bodies produced?
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Amanita virosa
botanist by day


Registered: 12/04/11
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Re: Chaga fruiting on the plate! [Re: Shu]
#19367153 - 01/04/14 07:58 AM (10 years, 27 days ago) |
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The lab stays at about 65 F. These are at five weeks from original clone. Never subbed and somehow completely free of bacteria.
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Jeff
Addict



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Neat stuff AV!
-------------------- Myco-tek
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drake89
Mushroom Magnate



Registered: 06/26/11
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Re: Chaga fruiting on the plate! [Re: Jeff]
#19367335 - 01/04/14 09:24 AM (10 years, 27 days ago) |
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wild! never seen a polypore pin on agar!
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: Chaga fruiting on the plate! [Re: drake89]
#19367438 - 01/04/14 09:59 AM (10 years, 26 days ago) |
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Whoa! Very fun!
--------------------
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Queen of Kings
Get on with the Fascination



Registered: 12/11/11
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Re: Chaga fruiting on the plate! [Re: Ganzig]
#19367450 - 01/04/14 10:02 AM (10 years, 26 days ago) |
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Very cool AV!
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Amanita virosa
botanist by day


Registered: 12/04/11
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What should I do y'all? Sub out little pieces of the fruit? Or multispore? My goal is to get vigorous Myc for plug innoc but I do not fully understand the life cycle.
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Forrester
aspiring sociopath


Registered: 02/05/13
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Loc: Northeast USA
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Looks like you've got an amazingly vigorous culture for your plugs - can't you just transfer parts of it that aren't fruits and use that to colonize your plugs? Sorry maybe I'm confused about what you're asking. That's an awesome culture though, mine sucks and grows super slow and doesn't do ANYTHING like that. So I wouldn't see any reason to go to spore, really...?
I was reading the other day chaga will often produce fruit bodies and spores when it's tree dies, as it's last chance to pass on genetics, so maybe your dish there felt threatened
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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OICU812
NC Tree Farm owner


Registered: 11/06/11
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Re: Chaga fruiting on the plate! [Re: Forrester]
#19368929 - 01/04/14 03:51 PM (10 years, 26 days ago) |
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Maybe try to take a few samples of the mycelium @ 5 o'clock in the picture above to get as far away from the spores as possible. Then get a few more samples at random areas and compare P2 plates, all done in a SAB, of course. It would be a shame to loose those original genetics!
-------------------- -------------- "Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin "Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin ----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson
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Forrester
aspiring sociopath


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Re: Chaga fruiting on the plate! [Re: OICU812]
#19369077 - 01/04/14 04:27 PM (10 years, 26 days ago) |
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Quote:
OICU812 said: Maybe try to take a few samples of the mycelium @ 5 o'clock in the picture above to get as far away from the spores as possible. Then get a few more samples at random areas and compare P2 plates, all done in a SAB, of course. It would be a shame to loose those original genetics!
Duh, the spores didn't even occur to me Good point.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Amanita virosa
botanist by day


Registered: 12/04/11
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Re: Chaga fruiting on the plate! [Re: Forrester]
#19369429 - 01/04/14 05:43 PM (10 years, 26 days ago) |
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Yea. The spores pretty much contaminate the original culture strain. This may not be a bad thing though. I with I would have subbed it prior.
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Forrester
aspiring sociopath


Registered: 02/05/13
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Quote:
Amanita virosa said: Yea. The spores pretty much contaminate the original culture strain. This may not be a bad thing though. I with I would have subbed it prior.
I would imagine a transfer of the culture (one as fast as yours) would outrun the spores before they could germinate and contaminate it, wouldn't you think?
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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OICU812
NC Tree Farm owner


Registered: 11/06/11
Posts: 1,064
Loc: Foothills of NC
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Re: Chaga fruiting on the plate! [Re: Forrester]
#19369857 - 01/04/14 07:12 PM (10 years, 26 days ago) |
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Quote:
Forrester said: I would imagine a transfer of the culture (one as fast as yours) would outrun the spores before they could germinate and contaminate it, wouldn't you think?
That sounds like it's worth a shot, Av, maybe try a non-nutritive agar to make the Mycelium run even faster, and make the agar a little on the dry/firm side by adding a little extra agar agar powder.
*/* P.S.: When you have what you think is clean mycelium, isolate some of it to sterile distilled water for long term storage.
Edited by OICU812 (01/05/14 09:16 AM)
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Amanita virosa
botanist by day


Registered: 12/04/11
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Re: Chaga fruiting on the plate! [Re: OICU812]
#19369942 - 01/04/14 07:27 PM (10 years, 26 days ago) |
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Good idea y'all. Will sub away tomorrow. Don't wanna get too carried away on this project. But it was cool to see the fruiting bodies for sure. I had never seem them in nature.
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Group
Working Together


Registered: 10/25/13
Posts: 115
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Amazing!!!
-------------------- We are starting a mushroom growing network to provide FREE and/or inexpensive gourmet and medicinal mushrooms to local communities accross the US and globally. We have the opportunity to feed and heal each other. We simply need to work together. By sharing our talents we enable ourselves and others to enjoy a world of abundance. We'd love for collaboration from YOU, PM me!
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woodland_jewel
In den Gärten Pharaos


Registered: 09/07/11
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Loc: Penna
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Re: Chaga fruiting on the plate! [Re: Group]
#19372693 - 01/05/14 12:27 PM (10 years, 25 days ago) |
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That's incredible, AV! Congrats!
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nycomyco
Stranger

Registered: 11/13/03
Posts: 651
Loc: PA
Last seen: 6 months, 10 days
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very cool! it's a privilege to see the fruitbodies. now who has the land to plant a birch forest- they grow very fast
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SnowArcher
Hood Rat


Registered: 12/30/13
Posts: 179
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Re: Chaga fruiting on the plate! [Re: nycomyco]
#19373691 - 01/05/14 04:18 PM (10 years, 25 days ago) |
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Nice!!!
Was that the only plate?
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Amanita virosa
botanist by day


Registered: 12/04/11
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Re: Chaga fruiting on the plate! [Re: SnowArcher]
#19373982 - 01/05/14 05:32 PM (10 years, 25 days ago) |
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All three plates are the same. I am honestly a little bummed that we didn't sub it before it started dropping spores. I was out of town and my partner saw so enthralled watching it he didn't think about the spores contaminating the isolate. It might not really matter since we are gonna try to o o grain then to four inch dowels to put into live birches.
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SnowArcher
Hood Rat


Registered: 12/30/13
Posts: 179
Last seen: 2 years, 10 months
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It is too bad that you didn't catch it but am sure it will be fine - let us know!
Have you inoculated live birch before? I have considered hitting a few of the trees around here for a personal source but am nervous that I will just end up harming some trees with no fruit...
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nanncee



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Loc: Utah
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Re: Chaga fruiting on the plate! [Re: SnowArcher]
#19377025 - 01/06/14 11:57 AM (10 years, 24 days ago) |
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Nothing to add (everyone said what I would have suggested) but man that is badass! Makes me want to track down a culture of chaga.
-------------------- I am a small scale farmer, come check out what we do. www.facebook.com/biocentricbros Check out our Youtube videos. www.youtube.com/biocentricbros
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deadmandave
Slime


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perhaps you cant pluck one of the fruits, open it up and take a culture from inside the chaga, much like culturing a wild found specimen.
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Amanita virosa
botanist by day


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Re: Chaga fruiting on the plate! [Re: SnowArcher]
#19378015 - 01/06/14 04:31 PM (10 years, 24 days ago) |
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Quote:
SnowArcher said: It is too bad that you didn't catch it but am sure it will be fine - let us know!
Have you inoculated live birch before? I have considered hitting a few of the trees around here for a personal source but am nervous that I will just end up harming some trees with no fruit...
Birch is a weed tree on my farm. We have 80 acres of them. So plenty to experiment with
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 6 hours
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Quote:
Amanita virosa said: It might not really matter since we are gonna try to o o grain then to four inch dowels to put into live birches.
I am trying to do this as well, but have never made dowels or inoculated trees/logs. Is there any reason you're using 4 inch dowels? I thought they were usually smaller? I'm really clueless when it comes to this type of growing... Also, how do you colonize the dowels, just with a wedge in a ball jar, or...?
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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forrest



Registered: 11/16/12
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Re: Chaga fruiting on the plate! [Re: Forrester]
#19380019 - 01/06/14 10:56 PM (10 years, 24 days ago) |
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you can just throw a bunch of dowels with some grains in a jar and treat it as a normal spawn jar except maybe longer incubation.
but for logs i think it's better so inoculate with sawdust, either by cutting ''>'' out of them or in between logs on the ends (sawdust sandwiched), it goes faster, less expensive, less chance of failure.
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
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Re: Chaga fruiting on the plate! [Re: forrest]
#19380057 - 01/06/14 11:04 PM (10 years, 24 days ago) |
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Quote:
forrest said: you can just throw a bunch of dowels with some grains in a jar and treat it as a normal spawn jar except maybe longer incubation.
but for logs i think it's better so inoculate with sawdust, either by cutting ''>'' out of them or in between logs on the ends (sawdust sandwiched), it goes faster, less expensive, less chance of failure.
Sounds good for the dowels - but we're talking live trees here, not logs so no sandwiching opportunities!
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Amanita virosa
botanist by day


Registered: 12/04/11
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Re: Chaga fruiting on the plate! [Re: Forrester] 1
#19381578 - 01/07/14 09:52 AM (10 years, 24 days ago) |
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Quote:
Forrester said:
Quote:
Amanita virosa said: It might not really matter since we are gonna try to o o grain then to four inch dowels to put into live birches.
I am trying to do this as well, but have never made dowels or inoculated trees/logs. Is there any reason you're using 4 inch dowels? I thought they were usually smaller? I'm really clueless when it comes to this type of growing... Also, how do you colonize the dowels, just with a wedge in a ball jar, or...?
 just use grain and hydrate the dowels by soaking them in coffee water. we soak for a couple of days, then bring to boil, steam dry, and then autoclave for one hour. then inoculate with grain. We sold 50,000 plus plugs last year. The usual size plug is 1 inch by 5/16th dia. But for spiking live trees we are going with something much longer.
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SnowArcher
Hood Rat


Registered: 12/30/13
Posts: 179
Last seen: 2 years, 10 months
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How many dowels are you putting into live trees? Do you think the strain would translate well to aspens?
Nice page! Got you on my list for future cultures.
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
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Quote:
Amanita virosa said: just use grain and hydrate the dowels by soaking them in coffee water. we soak for a couple of days, then bring to boil, steam dry, and then autoclave for one hour. then inoculate with grain. We sold 50,000 plus plugs last year. The usual size plug is 1 inch by 5/16th dia. But for spiking live trees we are going with something much longer.
Awesome man that's exactly what I was looking for, thanks so much for the info!
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Amanita virosa
botanist by day


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Re: Chaga fruiting on the plate! [Re: Forrester]
#19389638 - 01/08/14 05:48 PM (10 years, 22 days ago) |
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Quote:
Forrester said:
Quote:
Amanita virosa said: just use grain and hydrate the dowels by soaking them in coffee water. we soak for a couple of days, then bring to boil, steam dry, and then autoclave for one hour. then inoculate with grain. We sold 50,000 plus plugs last year. The usual size plug is 1 inch by 5/16th dia. But for spiking live trees we are going with something much longer.
Awesome man that's exactly what I was looking for, thanks so much for the info! 
Two other notes. Make sure you use spiral groove dowels and as you heat them up keep checking them. You are shooting for 25 percent sinking. In other words don't over hydrate then. For your first batch disk 24 hrs then boil. Sample periodically by scooping some out and seeing what percent sink. Be SURE you let them steam completely dry too. If they are too wet when you clave them the water comes out and settles in the bottom of the bag. Not good
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
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Quote:
Amanita virosa said: Two other notes. Make sure you use spiral groove dowels and as you heat them up keep checking them. You are shooting for 25 percent sinking. In other words don't over hydrate then. For your first batch disk 24 hrs then boil. Sample periodically by scooping some out and seeing what percent sink. Be SURE you let them steam completely dry too. If they are too wet when you clave them the water comes out and settles in the bottom of the bag. Not good
Kinda confused on the sinking thing... so I just take a bunch out of the boiling water and toss them into a different pot of cold water to see how many sink?
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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FrayedO
Newbie


Registered: 02/23/06
Posts: 9
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Do you still have this culture? I am looking to trade for a Reishi culture, or something, as I am interested into doing an experiment inoculating live birch trees.
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