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Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Cultivation Supplies, Injection Grain Bag

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Offlinedeachy
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Registered: 09/26/09
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Agar flesh time frame?
    #19365504 - 01/03/14 08:08 PM (10 years, 27 days ago)

Hey all,

Quick question. I have some beautiful mushrooms that i picked and I want to transfer flesh to a few agar plates, the problem is that I won't be able to prep my agar until next week...

How long do I have before the flesh is not usable? What is the longest I can wait to transfer?


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InvisibleMudaFuka
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Registered: 12/14/13
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Re: Agar flesh time frame? [Re: deachy]
    #19365623 - 01/03/14 08:31 PM (10 years, 27 days ago)

I asked a similar question about a week ago and the general consensus was you should clone your fruits within a couple hours of picking them. You might be abled to keep them a bit longer in a paper bag but im not 100% so you might wont to wait for someone more experienced to chime in.


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Offlinehushmush

Registered: 12/30/13
Posts: 165
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Re: Agar flesh time frame? [Re: deachy]
    #19365681 - 01/03/14 08:44 PM (10 years, 27 days ago)

You could make a LC and once you have your agar ready use that to clean up your LC


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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: Agar flesh time frame? [Re: hushmush]
    #19365711 - 01/03/14 08:51 PM (10 years, 27 days ago)

toss some dry grains in the blender and weigh out about 5 grams of flour and about 16 cc's of water (give or take) which will fit in the bottom of a half pint jar and sterilize for 30 minutes at 15 psi, keep your fruit in the paper bag till tommorow or whenever it's cool enough and drop your tissue in, you should have some nice myc by the time your readyto prep your agar, and you can use that recipe for however many jars you decide to do but find a container that will make pouring easier:thumbup:


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Offlinedeachy
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Registered: 09/26/09
Posts: 63
Last seen: 3 years, 6 months
Re: Agar flesh time frame? [Re: cronicr]
    #19369515 - 01/04/14 06:01 PM (10 years, 26 days ago)

Will the flesh of the fruit not produce myc if it's not used very soon after it's picked? What is the reason for not just storing the fruit for a few days and then completing agar dishes?

Can i not just use flesh from a fruit that is a few days old in agar and isolate the contams?


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