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Offlinebolbol
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Re: Question about multispores [Re: cronicr]
    #19366542 - 01/04/14 12:46 AM (10 years, 27 days ago)

what is so significant about rhizo growth of mycelium?
I had a search done and only results I got was that mycelium grows like that because of being in search of more nutrients and that its a bad sign??
my first set of jars inoculated with mexican cubes had normal growth and the new ones that i have with PES amazonian have the rhizo growth


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OfflinecronicrFacebook
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Re: Question about multispores [Re: bolbol]
    #19366551 - 01/04/14 12:53 AM (10 years, 27 days ago)

not a bad sign lol, it's just easier to see what your working with on plates, it's a bad sign if you stick a grain on cardboard where it goesright rhizzo because theres no food for it to eat or at least very minimal, in things like grain jars it's:thumbup:


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OfflineFert Nitty
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Re: Question about multispores [Re: cronicr]
    #19369646 - 01/04/14 06:27 PM (10 years, 26 days ago)

Quote:

cronicr said:
take it to the sab and take a piece from the middle,use a little less nutes in your agar to manipulate the rhizzo growth but generally they tend to take right off on agar anyway as brf is alot more nutritious then the agar:cool:





my agar plates are prepoured, next i will be making my own.


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OfflinecronicrFacebook
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Re: Question about multispores [Re: Fert Nitty]
    #19369649 - 01/04/14 06:28 PM (10 years, 26 days ago)

no worries at all you will still see good growth


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OfflineFert Nitty
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Re: Question about multispores [Re: cronicr]
    #19400731 - 01/10/14 07:17 PM (10 years, 20 days ago)

well i just got over my cold, so i just dropped a couple cc's out of my syringe into an agar plate in my s.a.b. so, just sticking it in my incubator should be the correct conditions for my culture?


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OfflinecronicrFacebook
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Re: Question about multispores [Re: Fert Nitty]
    #19400753 - 01/10/14 07:24 PM (10 years, 20 days ago)

YEP


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OfflineFert Nitty
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Re: Question about multispores [Re: cronicr]
    #19448198 - 01/20/14 03:17 PM (10 years, 10 days ago)

I just today spotted the first mycelial growth on my agar plate... I was beginning to think it was a dud. joy!


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OfflineFert Nitty
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Re: Question about multispores [Re: Fert Nitty]
    #19448203 - 01/20/14 03:17 PM (10 years, 10 days ago)

it is kind if fuzzy like mycelium lacking FAE.


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OfflinecronicrFacebook
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Re: Question about multispores [Re: Fert Nitty]
    #19448567 - 01/20/14 04:47 PM (10 years, 10 days ago)

they don't need fae at that point:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn

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OfflineFert Nitty
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Re: Question about multispores [Re: cronicr]
    #19448831 - 01/20/14 05:44 PM (10 years, 10 days ago)

oh i know, i was just describing the way it looked, like it was stretching out hard. it should grow fast from here now,right?


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Re: Question about multispores [Re: Fert Nitty]
    #19508729 - 02/01/14 11:33 PM (9 years, 11 months ago)

I realize this isn't ideal, but finally this is the growth I've seen so far from drops out of a spore syringe. Should I let it grow before doing anything with it?


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OfflineVYBE
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Re: Question about multispores [Re: bolbol]
    #19508793 - 02/01/14 11:58 PM (9 years, 11 months ago)

Quote:

bolbol said:
what is so significant about rhizo growth of mycelium?
I had a search done and only results I got was that mycelium grows like that because of being in search of more nutrients and that its a bad sign??
my first set of jars inoculated with mexican cubes had normal growth and the new ones that i have with PES amazonian have the rhizo growth



You want to isolate rhizo growth so you can have the strongest and fastest growing strain if I'm not mistaken. I'm just now getting into isolation and cloning on agar but isn't that what people are after?


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OfflineFert Nitty
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Re: Question about multispores [Re: VYBE]
    #19508800 - 02/02/14 12:00 AM (9 years, 11 months ago)

yea that's what we are after. I wait for to pieces of mycelium to touch and they make rhizo? or that is how I begin isolation at least is what I understand.


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OfflineVYBE
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Re: Question about multispores [Re: Fert Nitty]
    #19508855 - 02/02/14 12:18 AM (9 years, 11 months ago)

You should look up an isolation and cloning tek. Not too difficult to understand but it's too much information for me to try to explain right now. Let me find a good link....

Here's sorta what we're talking about
https://mycotopia.net/topic/25864-strain-isolation-can-cubensis-benefit-from-advanced-teks/

There's also some links to some info by RR that I thought I had book marked but i can't find them


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OfflineFert Nitty
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Re: Question about multispores [Re: VYBE]
    #19534780 - 02/07/14 10:43 AM (9 years, 11 months ago)

I used liquid from a spore syringe on this agar plate so it kind of made a mess and created uneven mycelium growth all over the place. I was wondering if I took a hunk (in a s.a.b. of course) of myc. with tweezers and set it in the center of another agar plate, it should be more even growth.



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InvisiblebodhisattaMDiscordReddit
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Re: Question about multispores [Re: Fert Nitty]
    #19534807 - 02/07/14 10:49 AM (9 years, 11 months ago)

Quote:

Fert Nitty said:
yea that's what we are after. I wait for to pieces of mycelium to touch and they make rhizo? or that is how I begin isolation at least is what I understand.




you're not necessarily after rhizo growth. On a petri dish rhizo growth is an indicator that you have good growth but it's not positive proof you have good genetics. Rhizo growth and tomentose growth are both dikaryotic mycelium IE the resultant of two monokaryons or hyphae or primary mycelium which is what a spore creates when it germinates. For practical purposes all the mycelium you see on your dish is dikaryotic mycelium which will usually show up tomentose on the first dish and then you may see rhizo growth on subsequent dishes. Rhizo growth is a good place to start when you're finding "Blind" isolates IE you don't know how they perform til you test them out. You could end up with shit or end up with gold and anything inbetween. If you're just starting out with agar don't even worry about isolates. Just put spores to agar transfer one or two times til you are sure you have clean growth and then use that, we call that sometimes limited MS or limited genetics. Then clone a good fruit from that grow. Worry about isolates when you've run out of fun with that.


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OfflineFert Nitty
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Re: Question about multispores [Re: bodhisatta]
    #19534833 - 02/07/14 10:57 AM (9 years, 11 months ago)

Im gonna transfer some of this to another plate.


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InvisiblebodhisattaMDiscordReddit
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Re: Question about multispores [Re: Fert Nitty]
    #19534867 - 02/07/14 11:06 AM (9 years, 11 months ago)

:thumbup:


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Offlinebolbol
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Re: Question about multispores [Re: bodhisatta]
    #19535186 - 02/07/14 12:38 PM (9 years, 11 months ago)

will it work if u dont use agar at all and just pick a fruit, get the tissue at the base of the stem and just throw it in a grain jar?


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Offlinesourdoug
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Re: Question about multispores [Re: bolbol]
    #19535322 - 02/07/14 01:21 PM (9 years, 11 months ago)

Seems to me throwing a chunk of mushroom in a grain jar to inoculate would have a high chance of contamination. 
Additionally, starting from just one little spot would probably mean slow colonization.


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